FastQCFastQC Report
Fri 10 Feb 2017
ERR1631371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358884
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.23433755753948352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7890.21984819607449763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6490.18083837674568942No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3910.1089488525540286No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3590.10003232242172957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCT250.00549136829.64
TCTAGCG604.305366E-827.74999828
CTAGCGG659.354153E-825.61538529
GCAATAC753.725945E-722.237
GCGAAAG603.717101E-521.58333218
AGCGAAA806.932696E-720.812517
CGCAATA806.932696E-720.812536
ATACCGT450.00382075920.5555556
TACACAG450.00382075920.5555555
CGGTCCA1001.2823875E-820.3510
GAGCGAA656.885234E-519.92307716
GTCCTAT851.2404307E-619.5882361
CTTATAC4700.019.28723537
GGTATCA5350.018.67291
ATAGGAC902.1438354E-618.53
CGGTTCT801.6116264E-518.512
CCGTCAA701.2162344E-418.523
GTTTTCG701.2162344E-418.528
GTTCAAA1306.91216E-1018.51
ATACGCT500.007025684718.58
Produced by FastQC (version 0.11.5)