##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631371.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358884
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.841310841386075	34.0	33.0	34.0	31.0	34.0
2	33.014809799266615	34.0	33.0	34.0	31.0	34.0
3	33.1192502312725	34.0	33.0	34.0	31.0	34.0
4	36.46545123215301	37.0	37.0	37.0	35.0	37.0
5	36.44074965727087	37.0	37.0	37.0	35.0	37.0
6	36.49072680866241	37.0	37.0	37.0	35.0	37.0
7	36.47469377291827	37.0	37.0	37.0	35.0	37.0
8	36.453255090781425	37.0	37.0	37.0	35.0	37.0
9	38.31436341547687	39.0	39.0	39.0	37.0	39.0
10	38.26570423869551	39.0	39.0	39.0	37.0	39.0
11	38.33471539550384	39.0	39.0	39.0	37.0	39.0
12	38.109341737162985	39.0	39.0	39.0	37.0	39.0
13	38.233735691755555	39.0	39.0	39.0	37.0	39.0
14	39.76509122724892	41.0	40.0	41.0	38.0	41.0
15	39.77227460683675	41.0	40.0	41.0	38.0	41.0
16	39.74561696815684	41.0	40.0	41.0	38.0	41.0
17	39.733362312056265	41.0	40.0	41.0	38.0	41.0
18	39.72573031954615	41.0	40.0	41.0	38.0	41.0
19	39.73525150187805	41.0	40.0	41.0	38.0	41.0
20	39.71835467727734	41.0	40.0	41.0	38.0	41.0
21	39.68550840940248	41.0	40.0	41.0	37.0	41.0
22	39.635046421685004	41.0	40.0	41.0	37.0	41.0
23	39.57203720422198	41.0	40.0	41.0	37.0	41.0
24	39.54526532249975	41.0	39.0	41.0	37.0	41.0
25	39.51385127227739	41.0	39.0	41.0	37.0	41.0
26	39.44116204678949	41.0	39.0	41.0	37.0	41.0
27	39.36248202761895	41.0	39.0	41.0	36.0	41.0
28	39.27776663211511	41.0	39.0	41.0	36.0	41.0
29	39.20176993123126	41.0	39.0	41.0	36.0	41.0
30	39.137595992019705	41.0	39.0	41.0	35.0	41.0
31	39.06900837039266	40.0	39.0	41.0	35.0	41.0
32	38.984574402871125	40.0	39.0	41.0	35.0	41.0
33	38.89108737084963	40.0	39.0	41.0	35.0	41.0
34	38.83739871379053	40.0	38.0	41.0	35.0	41.0
35	38.73276044627233	40.0	38.0	41.0	35.0	41.0
36	38.66823820510248	40.0	38.0	41.0	35.0	41.0
37	38.607951315745474	40.0	38.0	41.0	35.0	41.0
38	38.50096409982056	40.0	38.0	41.0	35.0	41.0
39	38.41141427313561	40.0	38.0	41.0	35.0	41.0
40	38.31874366090436	40.0	38.0	41.0	34.0	41.0
41	38.21168957100345	40.0	37.0	41.0	34.0	41.0
42	38.10884296875871	40.0	37.0	41.0	34.0	41.0
43	37.153311376377886	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	2.0
15	1.0
16	3.0
17	6.0
18	15.0
19	18.0
20	32.0
21	62.0
22	109.0
23	183.0
24	251.0
25	391.0
26	521.0
27	746.0
28	1165.0
29	1516.0
30	2099.0
31	2810.0
32	3574.0
33	4955.0
34	7387.0
35	10843.0
36	17705.0
37	34104.0
38	77410.0
39	192974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.98663635046422	16.444032054925824	12.38617492002987	27.18315667458009
2	20.50049598198861	18.927564338337735	32.61137303418375	27.96056664548991
3	20.813967744452246	20.081419065770557	28.357630877943844	30.74698231183335
4	15.486898273536854	15.064477658519188	31.789659054179065	37.65896501376489
5	16.889022636840874	33.95470402692792	32.05102484368208	17.105248492549123
6	36.35547976504943	31.828668873507876	15.517270204299995	16.298581157142696
7	31.971333355624658	28.46212146543173	19.00474805229545	20.561797126648166
8	29.87567013296776	30.341280190813748	18.64502178977051	21.13802788644799
9	28.197133335562462	13.484579975702454	17.9261265478539	40.39216014088118
10	19.454754182409914	24.212280291124706	29.456871858316337	26.876093668149043
11	38.06076615285161	20.52752421395214	18.801061067085744	22.6106485661105
12	24.150421863331882	24.02447587521316	26.049085498378304	25.776016763076647
13	32.653447910745534	17.189398245672695	23.177405512644754	26.979748330937014
14	24.98718248793482	18.957657627534243	22.225008637888564	33.83015124664237
15	28.58611696258401	24.361910812407352	19.74816375207588	27.303808472932754
16	27.766632115112404	23.41703725995029	21.940794239921534	26.87553638501577
17	26.12989155270227	24.29754461051482	22.85139487968257	26.721168957100343
18	26.468719697729632	22.0411052039099	24.215345348357687	27.274829750002787
19	26.985599803836337	23.541868681802477	23.63465632349171	25.837875190869475
20	27.545390711204735	21.9867700984162	23.825804438202873	26.64203475217619
21	28.653548221709524	22.102406348569453	22.954213617770645	26.289831811950382
22	28.747450429665296	21.93132042665597	22.13612197813221	27.18510716554653
23	27.241950045139934	22.21943580655588	22.956721391870357	27.581892756433835
24	26.56512967978511	22.73520134639605	23.539082266136134	27.160586707682704
25	27.0120707526666	22.07064120997314	23.01997302749635	27.89731500986391
26	27.257832614438094	22.81990838265289	23.105794630019727	26.816464372889293
27	27.58913743716633	21.85636584523133	22.671949710770054	27.882547006832294
28	26.508286800191705	22.43064611406471	23.81215100143779	27.24891608430579
29	25.898897693962397	22.652723442672283	24.475039288460895	26.973339574904426
30	25.718337958783337	23.570568763165813	24.46054992699591	26.25054335105494
31	26.835411999420426	23.231740618138453	22.878144470079466	27.054702912361655
32	25.630844506860157	22.678079825236008	24.269123170718114	27.42195249718572
33	25.77490219681011	22.243398981286433	24.670088385104936	27.31161043679852
34	26.88668204768114	21.950546694753736	24.127851896434503	27.034919361130616
35	26.119860456303428	22.304978767512623	25.0275855150968	26.547575261087147
36	24.7703993490933	22.422008225499045	25.295081419065767	27.51251100634188
37	25.82728681133737	21.21242518473936	25.633352280959866	27.32693572296341
38	24.755352704495046	20.818983292651662	26.813677957222946	27.611986045630342
39	24.984117430701843	20.522787307319355	27.287368620501333	27.20572664147747
40	24.138997559099877	20.739570446160876	27.61616566912986	27.50526632560939
41	22.931922292439896	20.957746792835568	29.12807480968781	26.982256105036722
42	23.51149675103933	20.785824946222178	28.559924655320383	27.142753647418104
43	22.258445625884686	20.80337936492014	29.309749111133403	27.62842589806177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.5
2	8.0
3	36.5
4	65.0
5	65.0
6	99.5
7	134.0
8	131.5
9	129.0
10	207.5
11	286.0
12	286.0
13	474.5
14	663.0
15	1118.5
16	1574.0
17	1460.5
18	1347.0
19	1347.0
20	1564.0
21	1781.0
22	1489.0
23	1197.0
24	1391.0
25	1585.0
26	1585.0
27	1893.0
28	2201.0
29	2810.0
30	3419.0
31	4211.5
32	5004.0
33	5004.0
34	5909.5
35	6815.0
36	8310.5
37	9806.0
38	11173.5
39	12541.0
40	12541.0
41	13780.5
42	15020.0
43	16289.5
44	17559.0
45	19646.0
46	21733.0
47	21733.0
48	24146.5
49	26560.0
50	27459.5
51	28359.0
52	29385.0
53	30411.0
54	30411.0
55	29320.5
56	28230.0
57	27504.0
58	26778.0
59	25563.5
60	24349.0
61	24349.0
62	23223.0
63	22097.0
64	20585.0
65	19073.0
66	17198.0
67	15323.0
68	15323.0
69	13357.0
70	11391.0
71	10046.5
72	8702.0
73	6986.5
74	5271.0
75	5271.0
76	4361.5
77	3452.0
78	2890.0
79	2328.0
80	1978.5
81	1629.0
82	1629.0
83	1285.0
84	941.0
85	756.0
86	571.0
87	447.0
88	323.0
89	323.0
90	248.0
91	173.0
92	105.0
93	37.0
94	24.0
95	11.0
96	11.0
97	7.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	358884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.94394473484182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54658257020385	62.42638584648926
2	5.171038650032876	7.130236058191953
3	1.4027136103234277	2.901258288868465
4	0.7065043648350071	1.9483679153643703
5	0.42035087604780474	1.44903237837411
6	0.27927129748172924	1.1552438939764744
7	0.21825752751885594	1.0533274440656255
8	0.15857697903167262	0.8746337982862245
9	0.1349076282422898	0.8370957659260513
>10	0.8326027955087387	11.403574276216782
>50	0.08950409990925398	4.174620735067959
>100	0.03847461308295179	4.009880835800542
>500	0.0012149877815668987	0.6363427633722886
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.23433755753948352	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	789	0.21984819607449763	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.18083837674568942	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	391	0.1089488525540286	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	359	0.10003232242172957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	2.7864156663434425E-4
16	0.0	0.0	0.0	8.359246999030327E-4	2.7864156663434425E-4
17	0.0	0.0	0.0	0.001114566266537377	2.7864156663434425E-4
18	0.0	0.0	0.0	0.002229132533074754	2.7864156663434425E-4
19	0.0	0.0	0.0	0.002507774099709098	2.7864156663434425E-4
20	0.0	0.0	0.0	0.002507774099709098	2.7864156663434425E-4
21	0.0	0.0	0.0	0.0030650572329777867	2.7864156663434425E-4
22	0.0	0.0	0.0	0.004458265066149508	2.7864156663434425E-4
23	0.0	0.0	0.0	0.005851472899321229	2.7864156663434425E-4
24	0.0	0.0	0.0	0.010031096398836392	2.7864156663434425E-4
25	0.0	0.0	0.0	0.011702945798642459	2.7864156663434425E-4
26	0.0	0.0	0.0	0.016718493998060656	2.7864156663434425E-4
27	0.0	0.0	0.0	0.04123895186188295	2.7864156663434425E-4
28	0.0	0.0	0.0	0.1454508977831277	2.7864156663434425E-4
29	0.0	0.0	0.0	0.2892299461664493	2.7864156663434425E-4
30	0.0	0.0	0.0	0.5037839524748944	2.7864156663434425E-4
31	0.0	0.0	0.0	1.1900781310952844	2.7864156663434425E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCT	25	0.005491368	29.6	4
TCTAGCG	60	4.305366E-8	27.749998	28
CTAGCGG	65	9.354153E-8	25.615385	29
GCAATAC	75	3.725945E-7	22.2	37
GCGAAAG	60	3.717101E-5	21.583332	18
AGCGAAA	80	6.932696E-7	20.8125	17
CGCAATA	80	6.932696E-7	20.8125	36
ATACCGT	45	0.003820759	20.555555	6
TACACAG	45	0.003820759	20.555555	5
CGGTCCA	100	1.2823875E-8	20.35	10
GAGCGAA	65	6.885234E-5	19.923077	16
GTCCTAT	85	1.2404307E-6	19.588236	1
CTTATAC	470	0.0	19.287235	37
GGTATCA	535	0.0	18.6729	1
ATAGGAC	90	2.1438354E-6	18.5	3
CGGTTCT	80	1.6116264E-5	18.5	12
CCGTCAA	70	1.2162344E-4	18.5	23
GTTTTCG	70	1.2162344E-4	18.5	28
GTTCAAA	130	6.91216E-10	18.5	1
ATACGCT	50	0.0070256847	18.5	8
>>END_MODULE