FastQCFastQC Report
Fri 10 Feb 2017
ERR1631370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903622
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36660.40570061375221056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33040.3656396147946819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27290.30200681258313766No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC20540.22730743607393358No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT14890.16478129129215535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.15393604848044867No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13050.1444187945844612No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10950.12117898855937548No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10590.11719502181221793No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9970.11033374574766883No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9690.10723510494432406No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9120.10092715759465795No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG9050.10015249739382176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT400.001930869323.1259
ACCGTTA450.003825184420.5555558
GGTATCA20150.019.3722081
TATCTAT1103.8487997E-818.5000025
GATATAA500.007033746718.51
TATTAAC500.007033746718.52
CCCGGTT500.007033746718.522
TACACAT2200.017.65909237
ATACACA4200.017.6190537
ATAGACG650.0015795817.0769233
CGAACTA1005.874992E-616.65000224
GCATCAG6700.016.56716315
CAGGACA7900.016.3924054
ATACTGG1151.2414002E-616.0869566
TCTATCG1151.2414002E-616.08695629
ATACCAT1059.338593E-615.8571446
TCTAGAT2700.015.7592582
GCTGCAT7050.015.7446812
TGCATCA7050.015.7446814
TCCATTA2003.6379788E-1215.7258
Produced by FastQC (version 0.11.5)