##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631370.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903622
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02828395058996	34.0	33.0	34.0	31.0	34.0
2	33.16642025094564	34.0	33.0	34.0	31.0	34.0
3	33.235433621580704	34.0	33.0	34.0	31.0	34.0
4	36.47561037690539	37.0	37.0	37.0	35.0	37.0
5	36.466969595693776	37.0	37.0	37.0	35.0	37.0
6	36.51932887866829	37.0	37.0	37.0	35.0	37.0
7	36.528098032141756	37.0	37.0	37.0	35.0	37.0
8	36.526798816319214	37.0	37.0	37.0	35.0	37.0
9	38.36696649705297	39.0	39.0	39.0	37.0	39.0
10	38.33542122701749	39.0	39.0	39.0	37.0	39.0
11	38.38597444506663	39.0	39.0	39.0	37.0	39.0
12	38.354026351726716	39.0	39.0	39.0	37.0	39.0
13	38.375701344146115	39.0	39.0	39.0	37.0	39.0
14	39.899542065155565	41.0	40.0	41.0	38.0	41.0
15	39.88778272330687	41.0	40.0	41.0	38.0	41.0
16	39.84636164236816	41.0	40.0	41.0	38.0	41.0
17	39.84703781005774	41.0	40.0	41.0	38.0	41.0
18	39.78767781218253	41.0	40.0	41.0	38.0	41.0
19	39.80183860065382	41.0	40.0	41.0	38.0	41.0
20	39.77195331676298	41.0	40.0	41.0	38.0	41.0
21	39.74509474094256	41.0	40.0	41.0	37.0	41.0
22	39.67308011535797	41.0	40.0	41.0	37.0	41.0
23	39.6075726354604	41.0	40.0	41.0	37.0	41.0
24	39.56787019351012	41.0	40.0	41.0	37.0	41.0
25	39.5564815819004	41.0	40.0	41.0	37.0	41.0
26	39.439357386163685	41.0	39.0	41.0	36.0	41.0
27	39.393473155810725	41.0	39.0	41.0	36.0	41.0
28	39.29858060118058	41.0	39.0	41.0	35.0	41.0
29	39.22688579959319	41.0	39.0	41.0	35.0	41.0
30	39.15013357355177	41.0	39.0	41.0	35.0	41.0
31	39.100374935537204	41.0	39.0	41.0	35.0	41.0
32	39.01102230800047	41.0	39.0	41.0	35.0	41.0
33	38.909489808791726	41.0	39.0	41.0	35.0	41.0
34	38.8361837139866	41.0	38.0	41.0	35.0	41.0
35	38.71343437853439	40.0	38.0	41.0	35.0	41.0
36	38.64285287432134	40.0	38.0	41.0	35.0	41.0
37	38.55589505346262	40.0	38.0	41.0	35.0	41.0
38	38.47117268061203	40.0	38.0	41.0	35.0	41.0
39	38.35576823052117	40.0	38.0	41.0	35.0	41.0
40	38.2216214301998	40.0	37.0	41.0	34.0	41.0
41	38.10078771875851	40.0	37.0	41.0	34.0	41.0
42	37.938436647182115	40.0	37.0	41.0	34.0	41.0
43	37.10404461157431	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	1.0
13	3.0
14	3.0
15	0.0
16	7.0
17	12.0
18	13.0
19	28.0
20	67.0
21	116.0
22	236.0
23	372.0
24	649.0
25	1073.0
26	1523.0
27	2104.0
28	3031.0
29	4206.0
30	5624.0
31	7147.0
32	8953.0
33	11932.0
34	18144.0
35	28231.0
36	42698.0
37	81456.0
38	172755.0
39	513235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.208308341319714	17.337005960456917	11.976468036413456	26.478217661809918
2	20.70168720991742	19.42880983420058	32.31926624185777	27.55023671402423
3	21.332149947655104	21.218606895361113	28.035948659948517	29.413294497035263
4	15.523969093271301	15.191750532855552	31.779217416131967	37.50506295774118
5	16.96373040939685	34.31279893583822	30.95298697906868	17.77048367569625
6	35.571621762197026	32.01626343758784	15.684987749302254	16.727127050912884
7	30.91679928111533	28.854875157975346	19.622253552923677	20.60607200798564
8	29.467299379607848	30.029149356700035	19.103673881335336	21.399877382356784
9	28.061733778062063	13.810420729021649	19.051992979365266	39.07585251355102
10	20.690620635619762	24.17548488195285	28.711341689334702	26.422552793092684
11	37.66475362485641	20.934749264626138	19.274209791262276	22.126287319255177
12	24.565470960202383	24.524192638072115	25.446812937268014	25.46352346445748
13	31.26705635763627	17.651849999225337	24.132989236649838	26.948104406488554
14	24.279842677579786	19.935437605547452	22.939791195876154	32.84492852099661
15	27.89994046183028	25.051514903355603	20.72149637791023	26.327048256903886
16	26.958728317814305	23.857320870895133	22.266168818377597	26.917781992912964
17	25.787995422864867	24.77031325045207	23.258065872676852	26.18362545400621
18	25.630075407637264	22.032332103468043	25.19383104882351	27.14376144007118
19	26.21450119629668	23.67450106349779	24.33174491103581	25.779252829169717
20	26.288536578348026	22.73417424542563	24.438094690036323	26.53919448619002
21	28.9449570727583	22.84860262366343	23.49677188027737	24.709668423300894
22	28.270892032287836	22.778883205588176	23.07646338845225	25.873761373671734
23	26.389131738713758	22.87604772792163	23.935561551179585	26.79925898218503
24	26.147991084767746	23.695527554663343	23.86119417189931	26.295287188669597
25	26.04020265110854	22.834326742819453	24.097686864640302	27.027783741431705
26	26.5567903393233	23.93190958166136	24.39449238730354	25.116807691711802
27	27.424963092974718	22.531434604292503	23.55310074345246	26.490501559280315
28	25.35816967714376	22.93370458001244	25.221829481796593	26.486296261047208
29	25.310915404892754	22.91555539816428	25.578615837153144	26.194913359789822
30	24.639506342253732	23.6612211743406	25.648556586714356	26.05071589669132
31	25.80138597776504	23.5765618809635	24.07599637901689	26.54605576225457
32	24.90432946519673	23.087087299778005	24.820998160735353	27.18758507428991
33	25.051293571869653	22.855795896956913	25.196597692397926	26.896312838775504
34	26.13282987797995	22.880806354869623	24.953907718050246	26.032456049100176
35	26.081702304724764	22.605801983572775	25.755570360172726	25.55692535152973
36	24.597121362693695	23.343057163282875	25.020639161065134	27.039182312958292
37	24.54355914309302	22.108027471664034	26.212177215694172	27.136236169548773
38	24.468306437868932	21.129078309293046	27.48693590904161	26.915679343796413
39	24.01026092768879	21.26707849078486	27.587088406435434	27.13557217509091
40	23.86252216081503	21.200900376484856	28.536047152459766	26.400530310240345
41	22.291953936491144	21.52194169685997	29.47725929647574	26.708845070173147
42	22.82303883703584	22.18737480937826	28.863507085927527	26.12607926765838
43	21.55027212706198	20.713307112929964	29.683872238613045	28.05254852139501
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	452.0
1	349.0
2	246.0
3	481.5
4	717.0
5	717.0
6	863.5
7	1010.0
8	985.5
9	961.0
10	1450.0
11	1939.0
12	1939.0
13	3209.5
14	4480.0
15	6192.0
16	7904.0
17	7094.5
18	6285.0
19	6285.0
20	6817.5
21	7350.0
22	5808.0
23	4266.0
24	4624.5
25	4983.0
26	4983.0
27	5659.5
28	6336.0
29	7696.0
30	9056.0
31	11036.5
32	13017.0
33	13017.0
34	16473.0
35	19929.0
36	24143.5
37	28358.0
38	31927.5
39	35497.0
40	35497.0
41	38712.0
42	41927.0
43	44748.0
44	47569.0
45	50452.0
46	53335.0
47	53335.0
48	56933.5
49	60532.0
50	62859.5
51	65187.0
52	67876.5
53	70566.0
54	70566.0
55	69187.0
56	67808.0
57	65510.5
58	63213.0
59	59952.0
60	56691.0
61	56691.0
62	54184.0
63	51677.0
64	47845.0
65	44013.0
66	39114.5
67	34216.0
68	34216.0
69	29569.0
70	24922.0
71	22557.5
72	20193.0
73	18595.0
74	16997.0
75	16997.0
76	15535.5
77	14074.0
78	11367.0
79	8660.0
80	6060.0
81	3460.0
82	3460.0
83	2714.0
84	1968.0
85	1842.5
86	1717.0
87	1560.0
88	1403.0
89	1403.0
90	983.0
91	563.0
92	330.5
93	98.0
94	62.5
95	27.0
96	27.0
97	20.0
98	13.0
99	10.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	903622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.998396597613024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.32045034788553	41.039211565321004
2	6.183362664308156	5.812162616080557
3	1.8858750340222354	2.658993083475419
4	0.9758761495995378	1.8345845723612246
5	0.6261864233844983	1.4714878935132736
6	0.4244535017587468	1.196918040774193
7	0.3149591558324438	1.0361802722503821
8	0.23462685666807834	0.8821668849710121
9	0.19118011828825077	0.8086643118800801
>10	1.4426574053892596	13.581695438699079
>50	0.19865295204285943	6.555080698156644
>100	0.18709178587232722	17.365814466037467
>500	0.012504242939510328	3.7509467336940703
>1k	0.0021233620085960936	2.0060934227856544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3666	0.40570061375221056	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3304	0.3656396147946819	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2729	0.30200681258313766	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2054	0.22730743607393358	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1489	0.16478129129215535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.15393604848044867	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1305	0.1444187945844612	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1095	0.12117898855937548	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1059	0.11719502181221793	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	997	0.11033374574766883	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	969	0.10723510494432406	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	912	0.10092715759465795	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	905	0.10015249739382176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.2133148595319724E-4	0.0	0.0	0.0	0.0
6	2.2133148595319724E-4	0.0	0.0	0.0	0.0
7	2.2133148595319724E-4	0.0	0.0	0.0	0.0
8	2.2133148595319724E-4	0.0	0.0	0.0	0.0
9	2.2133148595319724E-4	0.0	0.0	3.3199722892979586E-4	0.0
10	2.2133148595319724E-4	0.0	0.0	5.533287148829931E-4	0.0
11	2.2133148595319724E-4	0.0	0.0	7.746602008361904E-4	0.0
12	2.2133148595319724E-4	0.0	0.0	9.959916867893876E-4	0.0
13	2.2133148595319724E-4	0.0	0.0	0.0012173231727425847	1.1066574297659862E-4
14	2.2133148595319724E-4	0.0	0.0	0.001438654658695782	1.1066574297659862E-4
15	2.2133148595319724E-4	0.0	0.0	0.0015493204016723808	1.1066574297659862E-4
16	2.2133148595319724E-4	0.0	0.0	0.0018813176306021766	2.2133148595319724E-4
17	2.2133148595319724E-4	0.0	0.0	0.0030986408033447615	2.2133148595319724E-4
18	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.00320930654632136	2.2133148595319724E-4
19	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.0036519695182277547	2.2133148595319724E-4
20	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.004426629719063945	3.3199722892979586E-4
21	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.006086615863712924	3.3199722892979586E-4
22	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.00885325943812789	3.3199722892979586E-4
23	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.014165215101004623	3.3199722892979586E-4
24	3.3199722892979586E-4	1.1066574297659862E-4	0.0	0.021247822651506935	3.3199722892979586E-4
25	6.639944578595917E-4	1.1066574297659862E-4	0.0	0.02755577000117306	3.3199722892979586E-4
26	6.639944578595917E-4	1.1066574297659862E-4	0.0	0.04647961205017142	3.3199722892979586E-4
27	6.639944578595917E-4	1.1066574297659862E-4	0.0	0.12638027847927563	3.3199722892979586E-4
28	7.746602008361904E-4	1.1066574297659862E-4	0.0	0.3497037478060516	3.3199722892979586E-4
29	7.746602008361904E-4	1.1066574297659862E-4	0.0	0.6263681052475482	3.3199722892979586E-4
30	7.746602008361904E-4	1.1066574297659862E-4	0.0	1.0159115205251754	3.3199722892979586E-4
31	7.746602008361904E-4	1.1066574297659862E-4	0.0	1.732582872041628	3.3199722892979586E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	40	0.0019308693	23.125	9
ACCGTTA	45	0.0038251844	20.555555	8
GGTATCA	2015	0.0	19.372208	1
TATCTAT	110	3.8487997E-8	18.500002	5
GATATAA	50	0.0070337467	18.5	1
TATTAAC	50	0.0070337467	18.5	2
CCCGGTT	50	0.0070337467	18.5	22
TACACAT	220	0.0	17.659092	37
ATACACA	420	0.0	17.61905	37
ATAGACG	65	0.00157958	17.076923	3
CGAACTA	100	5.874992E-6	16.650002	24
GCATCAG	670	0.0	16.567163	15
CAGGACA	790	0.0	16.392405	4
ATACTGG	115	1.2414002E-6	16.086956	6
TCTATCG	115	1.2414002E-6	16.086956	29
ATACCAT	105	9.338593E-6	15.857144	6
TCTAGAT	270	0.0	15.759258	2
GCTGCAT	705	0.0	15.74468	12
TGCATCA	705	0.0	15.74468	14
TCCATTA	200	3.6379788E-12	15.725	8
>>END_MODULE