FastQCFastQC Report
Fri 10 Feb 2017
ERR1631369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687143
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC48450.2871718639143214No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA48180.2855715253538082No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC47990.28444536118159514No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC36810.21817949041663928No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC30100.17840811359795822No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC29330.17384418511056857No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC26480.15695172252737322No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG24550.14551226540963036No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT23700.14047416253394052No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT23190.1374513008085266No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC23020.13644368023338863No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT22130.13116849016354867No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG21570.12784926944544714No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC20810.12334461275659503No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC20790.12322606915951997No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA18260.10823030412952547No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC18180.10775612974122525No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG17630.10449618082166125No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC17380.10301438585822303No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT17370.10295511405968552No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG17290.1024809396713853No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG17180.10182894988747249No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG17030.10093987290940958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA250.00549706529.59999833
GGTATCA10400.023.3028851
TGCATCA11200.021.47321314
AGTCGGT8850.020.90395411
GCAGTCG9600.019.8489579
CAGTCGG9750.019.35384610
GCATCAG12350.019.32388715
GACAGGC13100.019.2061067
CTTATAC12450.019.0200837
CTCTATG16700.018.9431131
CGCAAGA4500.018.9111122
CGCCTTA6850.018.9051125
ATCAGAA12850.018.71595417
CGATTAG609.23926E-418.519
CTGCATC12800.018.513
GTTCAAA5150.018.3203871
ACAGGCT14050.018.3024928
TCTATGG17500.018.0771432
CTTAGAA2050.018.0487822
CATCAGA13950.017.77060916
Produced by FastQC (version 0.11.5)