##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631369.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1687143
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.047115745375464	34.0	33.0	34.0	31.0	34.0
2	33.19455138064764	34.0	33.0	34.0	31.0	34.0
3	33.277745869792895	34.0	34.0	34.0	31.0	34.0
4	36.51459479131289	37.0	37.0	37.0	35.0	37.0
5	36.50688945750301	37.0	37.0	37.0	35.0	37.0
6	36.55640274712932	37.0	37.0	37.0	35.0	37.0
7	36.557958631840926	37.0	37.0	37.0	35.0	37.0
8	36.54787057172984	37.0	37.0	37.0	35.0	37.0
9	38.41059056641909	39.0	39.0	39.0	37.0	39.0
10	38.360971180273395	39.0	39.0	39.0	37.0	39.0
11	38.425775408486416	39.0	39.0	39.0	37.0	39.0
12	38.380285488544835	39.0	39.0	39.0	37.0	39.0
13	38.40226228600658	39.0	39.0	39.0	37.0	39.0
14	39.955306100312775	41.0	40.0	41.0	38.0	41.0
15	39.963429300302344	41.0	40.0	41.0	38.0	41.0
16	39.94467214693716	41.0	40.0	41.0	38.0	41.0
17	39.91184861034305	41.0	40.0	41.0	38.0	41.0
18	39.868311696163275	41.0	40.0	41.0	38.0	41.0
19	39.892916012454194	41.0	40.0	41.0	38.0	41.0
20	39.8764206709212	41.0	40.0	41.0	38.0	41.0
21	39.85233676102144	41.0	40.0	41.0	38.0	41.0
22	39.80458147293976	41.0	40.0	41.0	38.0	41.0
23	39.753001375698446	41.0	40.0	41.0	38.0	41.0
24	39.731709760227794	41.0	40.0	41.0	37.0	41.0
25	39.70210231142233	41.0	40.0	41.0	37.0	41.0
26	39.62742636516288	41.0	40.0	41.0	37.0	41.0
27	39.58283263481519	41.0	40.0	41.0	37.0	41.0
28	39.49754229487364	41.0	40.0	41.0	37.0	41.0
29	39.452234339353566	41.0	39.0	41.0	37.0	41.0
30	39.39674408156274	41.0	39.0	41.0	36.0	41.0
31	39.376845353357716	41.0	39.0	41.0	36.0	41.0
32	39.31793629822724	41.0	39.0	41.0	36.0	41.0
33	39.24482572016717	41.0	39.0	41.0	35.0	41.0
34	39.191572380053145	41.0	39.0	41.0	35.0	41.0
35	39.092187799137356	41.0	39.0	41.0	35.0	41.0
36	39.0431101572303	41.0	39.0	41.0	35.0	41.0
37	38.98271989985437	40.0	39.0	41.0	35.0	41.0
38	38.906436502418586	40.0	39.0	41.0	35.0	41.0
39	38.8199927332775	40.0	38.0	41.0	35.0	41.0
40	38.726198668399775	40.0	38.0	41.0	35.0	41.0
41	38.63161391772956	40.0	38.0	41.0	35.0	41.0
42	38.51123408033581	40.0	37.0	41.0	35.0	41.0
43	37.68005912954622	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	9.0
18	18.0
19	33.0
20	68.0
21	145.0
22	265.0
23	462.0
24	779.0
25	1224.0
26	1812.0
27	2615.0
28	3827.0
29	5098.0
30	7031.0
31	9311.0
32	12379.0
33	17654.0
34	28097.0
35	44972.0
36	70932.0
37	131416.0
38	344372.0
39	1004616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.228141894314824	16.13242031054866	11.250972798393496	28.38846499674301
2	20.63417268127242	17.79398663895117	32.490606901726764	29.081233778049636
3	20.965679850492815	19.93921084341991	27.240370259071106	31.854739047016167
4	14.75998181541221	14.224164756632959	32.329506153301764	38.686347274653066
5	16.988897799415938	34.669141856973596	31.816093834369703	16.525866509240767
6	38.03234224958999	32.10089482634252	14.823817542437126	15.042945381630366
7	32.89270678300535	27.522978194498037	19.040531833993917	20.543783188502694
8	30.30169938173587	30.500496994030733	17.91875377487267	21.279049849360725
9	28.217821488753476	13.262005650973272	17.01906714487154	41.501105715401714
10	19.27471471001569	23.90674649392494	29.819167669841857	26.99937112621752
11	40.07852327870252	19.890489425022064	18.483910373927994	21.547076922347426
12	25.30982850890529	22.549363035617016	25.624680302736635	26.516128152741054
13	32.45172460188614	16.666459215371784	22.87689899433539	28.004917188406676
14	24.29047211765689	18.151869758520768	21.793232701673777	35.76442542214856
15	28.61079351305728	24.214900574521543	19.48429978964439	27.69000612277679
16	28.757906117027428	22.93379992093142	20.540345424187517	27.767948537853638
17	27.235984145979327	23.527347711486225	21.79862643534069	27.43804170719376
18	27.61639054899318	21.091395335190914	23.561251180249688	27.73096293556622
19	27.665349054585175	22.730379108350625	21.64256378979138	27.961708047272815
20	27.469752119411332	21.780311449592595	22.354181003032938	28.39575542796313
21	30.060107530896907	21.89440966177734	21.687847443874052	26.357635363451703
22	29.605433564315533	21.693715351929267	21.21343596837968	27.48741511537552
23	27.79924404748145	21.701479957537686	21.94828772664795	28.550988268332915
24	27.551310114198973	22.373266522162023	21.91989653514847	28.15552682849053
25	27.550895211609213	21.665027801437102	22.06090414386925	28.723172843084434
26	28.524849405177864	22.636136948675954	22.17600997662913	26.66300366951705
27	29.369828165128858	21.1760354635025	21.707407137391435	27.746729233977202
28	27.28292741042105	21.58026912952844	22.813774528892928	28.323028931157584
29	26.513401650008326	21.756365642983432	23.723833723638126	28.00639898337011
30	25.739963950892133	23.20378296326986	23.583833735492487	27.472419350345522
31	27.681767342780073	22.638270733423308	21.939693315860005	27.740268607936613
32	26.77360484558807	21.90490077011848	22.77844853696456	28.543045847328884
33	26.748947777396463	21.810777154040885	22.77649256761282	28.66378250094983
34	27.931479430018673	21.927838956152502	22.782953193653412	27.35772842017541
35	27.792901965037935	21.677237791935834	23.369803270973474	27.160056972052754
36	26.563723406966687	23.00071778148029	22.682191136139615	27.753367675413404
37	27.445509953809488	21.155468149409977	23.608905706273863	27.790116190506676
38	26.29225856966481	20.958982137258076	24.477296826647176	28.271462466429938
39	26.489040940809407	21.056839876643533	24.515586408502422	27.938532774044646
40	25.890336503781835	20.531513926205424	25.40525610455071	28.172893465462028
41	24.327931894332607	21.085645970732774	26.763291552642542	27.823130582292077
42	25.547389877443706	21.061640892325073	26.34192833683926	27.04904089339196
43	23.868990358256532	19.70965116768407	27.091775860137524	29.32958261392188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	10.5
2	12.0
3	37.0
4	62.0
5	62.0
6	82.0
7	102.0
8	121.0
9	140.0
10	194.0
11	248.0
12	248.0
13	404.0
14	560.0
15	932.0
16	1304.0
17	1292.5
18	1281.0
19	1281.0
20	1607.5
21	1934.0
22	2042.5
23	2151.0
24	2988.5
25	3826.0
26	3826.0
27	5063.5
28	6301.0
29	8145.5
30	9990.0
31	13193.5
32	16397.0
33	16397.0
34	21427.5
35	26458.0
36	34677.5
37	42897.0
38	50052.0
39	57207.0
40	57207.0
41	63891.0
42	70575.0
43	77666.0
44	84757.0
45	91492.0
46	98227.0
47	98227.0
48	107075.5
49	115924.0
50	122330.0
51	128736.0
52	135378.0
53	142020.0
54	142020.0
55	140843.0
56	139666.0
57	133630.0
58	127594.0
59	123845.5
60	120097.0
61	120097.0
62	118733.5
63	117370.0
64	110763.5
65	104157.0
66	94023.0
67	83889.0
68	83889.0
69	70244.5
70	56600.0
71	49876.5
72	43153.0
73	37461.0
74	31769.0
75	31769.0
76	28630.0
77	25491.0
78	19925.0
79	14359.0
80	10045.0
81	5731.0
82	5731.0
83	4218.5
84	2706.0
85	2247.0
86	1788.0
87	1387.0
88	986.0
89	986.0
90	757.5
91	529.0
92	311.0
93	93.0
94	59.0
95	25.0
96	25.0
97	21.0
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1687143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.35403543385902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9575469378685	39.844791765655565
2	7.570156274252364	7.018145843530885
3	2.170852059348034	3.0188325984205377
4	1.0829069202201338	2.007884230058209
5	0.6225597749191643	1.4429078933149118
6	0.39653223410339256	1.102852153817756
7	0.2838160587715906	0.9209213751497567
8	0.2218677336262648	0.8227571828913498
9	0.15493494431390184	0.6463673908802622
>10	1.191697644237284	11.262134008157517
>50	0.1673972647906262	5.435449174779207
>100	0.15569078928001373	15.219346238525471
>500	0.016584684442153188	5.187091787389408
>1k	0.0074566798267045355	6.070518357429167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4845	0.2871718639143214	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	4818	0.2855715253538082	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	4799	0.28444536118159514	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	3681	0.21817949041663928	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	3010	0.17840811359795822	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2933	0.17384418511056857	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2648	0.15695172252737322	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	2455	0.14551226540963036	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2370	0.14047416253394052	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	2319	0.1374513008085266	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2302	0.13644368023338863	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	2213	0.13116849016354867	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	2157	0.12784926944544714	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	2081	0.12334461275659503	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	2079	0.12322606915951997	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1826	0.10823030412952547	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	1818	0.10775612974122525	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1763	0.10449618082166125	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	1738	0.10301438585822303	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1737	0.10295511405968552	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1729	0.1024809396713853	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	1718	0.10182894988747249	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1703	0.10093987290940958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.927179853752764E-5
2	0.0	5.927179853752764E-5	0.0	5.927179853752764E-5	5.927179853752764E-5
3	0.0	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
4	0.0	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
5	5.927179853752764E-5	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
6	5.927179853752764E-5	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
7	5.927179853752764E-5	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
8	5.927179853752764E-5	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
9	5.927179853752764E-5	5.927179853752764E-5	0.0	1.1854359707505528E-4	5.927179853752764E-5
10	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.1854359707505528E-4	5.927179853752764E-5
11	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.1854359707505528E-4	5.927179853752764E-5
12	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.1854359707505528E-4	5.927179853752764E-5
13	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.1854359707505528E-4	5.927179853752764E-5
14	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.1854359707505528E-4	5.927179853752764E-5
15	5.927179853752764E-5	1.1854359707505528E-4	0.0	1.7781539561258293E-4	5.927179853752764E-5
16	5.927179853752764E-5	1.7781539561258293E-4	0.0	2.3708719415011057E-4	1.1854359707505528E-4
17	1.1854359707505528E-4	2.3708719415011057E-4	0.0	4.149025897626935E-4	1.1854359707505528E-4
18	1.1854359707505528E-4	2.3708719415011057E-4	0.0	4.149025897626935E-4	1.7781539561258293E-4
19	1.1854359707505528E-4	2.3708719415011057E-4	0.0	5.927179853752765E-4	1.7781539561258293E-4
20	1.1854359707505528E-4	2.3708719415011057E-4	0.0	8.29805179525387E-4	1.7781539561258293E-4
21	1.1854359707505528E-4	2.3708719415011057E-4	0.0	0.0014225231649006634	1.7781539561258293E-4
22	2.3708719415011057E-4	2.3708719415011057E-4	0.0	0.0020745129488134676	1.7781539561258293E-4
23	2.3708719415011057E-4	2.3708719415011057E-4	0.0	0.0033784925166390756	1.7781539561258293E-4
24	2.3708719415011057E-4	2.3708719415011057E-4	0.0	0.005690092659602654	1.7781539561258293E-4
25	2.3708719415011057E-4	2.3708719415011057E-4	0.0	0.007231159421578372	1.7781539561258293E-4
26	2.9635899268763824E-4	2.3708719415011057E-4	0.0	0.011261641722130252	1.7781539561258293E-4
27	2.9635899268763824E-4	2.3708719415011057E-4	0.0	0.024657068191611498	1.7781539561258293E-4
28	2.9635899268763824E-4	2.3708719415011057E-4	0.0	0.0861811950735652	1.7781539561258293E-4
29	2.9635899268763824E-4	2.3708719415011057E-4	0.0	0.18392039086194828	1.7781539561258293E-4
30	2.9635899268763824E-4	2.3708719415011057E-4	0.0	0.3069093728273181	1.7781539561258293E-4
31	3.5563079122516585E-4	2.3708719415011057E-4	0.0	0.7100168746810437	1.7781539561258293E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	25	0.005497065	29.599998	33
GGTATCA	1040	0.0	23.302885	1
TGCATCA	1120	0.0	21.473213	14
AGTCGGT	885	0.0	20.903954	11
GCAGTCG	960	0.0	19.848957	9
CAGTCGG	975	0.0	19.353846	10
GCATCAG	1235	0.0	19.323887	15
GACAGGC	1310	0.0	19.206106	7
CTTATAC	1245	0.0	19.02008	37
CTCTATG	1670	0.0	18.943113	1
CGCAAGA	450	0.0	18.911112	2
CGCCTTA	685	0.0	18.90511	25
ATCAGAA	1285	0.0	18.715954	17
CGATTAG	60	9.23926E-4	18.5	19
CTGCATC	1280	0.0	18.5	13
GTTCAAA	515	0.0	18.320387	1
ACAGGCT	1405	0.0	18.302492	8
TCTATGG	1750	0.0	18.077143	2
CTTAGAA	205	0.0	18.048782	2
CATCAGA	1395	0.0	17.770609	16
>>END_MODULE