FastQCFastQC Report
Fri 10 Feb 2017
ERR1631368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22428
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA390.17388978063135366No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC300.13376136971642588No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC290.12930265739254504No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC270.1203852327447833No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT230.10255038344925986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC701.1529236E-418.537
CTCTTAT1652.2338463E-713.45454637
TCTTATA1050.00250677712.33333436
TCTCTTA2401.8144328E-610.02083437
GTCTCTT2801.2923045E-58.58928636
TGTCTCT3155.6687586E-57.634920635
CCTGTCT2250.0098405667.437
CTGTCTC3602.953074E-46.680555334
Produced by FastQC (version 0.11.5)