FastQCFastQC Report
Fri 10 Feb 2017
ERR1631367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213506
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105144.924451771847161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74423.485616329283486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73063.421917885211657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26381.2355624666285725No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA14540.6810113064738227No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.5100559234869277No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9370.4388635448184126No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA8750.4098245482562551No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.39108971176454055No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7420.34753121692130434No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7390.34612610418442574No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA6580.3081880602887038No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC5980.28008580555113205No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.27914906372654635No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.2730602418667391No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT5780.2707183873052748No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.26978164548068906No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT5750.2693132745683962No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT5610.2627560817962961No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC5530.2590091144979532No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT5300.24823658351521738No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA5300.24823658351521738No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA4880.22856500519891715No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.22575477972515992No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA4450.208425055970324No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT4280.20046275046134537No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA4000.18734836491714518No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.1868799940048523No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT3590.1681451575131378No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG3510.1643981902147949No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3460.16205633565333058No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.16111959382874486No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.15971448109186628No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT3260.1526889174074733No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.15175217558288762No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA3100.14519498281078752No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.14378987007390892No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC3040.14238475733703035No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.13254896817888023No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA2670.1250550335821944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2640.12364992084531583No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT2630.12318154993302295No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC2580.12083969537155864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2580.12083969537155864No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2550.11943458263468006No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT2490.11662435716092288No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT2480.11615598624863002No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA2410.11287738986257996No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2380.11147227712570139No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2370.11100390621340851No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT2370.11100390621340851No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC2330.10913042256423706No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2320.10866205165194422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2280.10678856800277274No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT2240.10491508435360129No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT2210.1035099716167227No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2200.10304160070442984No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA2180.10210485887984413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTT200.001838581837.010
TGTTACG251.2302618E-436.99999616
ACCGTTA401.5920014E-632.3758
GCCGTTT401.5920014E-632.37526
TATTGTT303.5897543E-430.83333413
CGTTATT303.5897543E-430.83333410
AATTTCG250.0054864529.59999823
CTCTTTT250.0054864529.59999836
TACCTTA250.0054864529.59999831
CAGGAGG250.0054864529.5999985
GCTACCT250.0054864529.59999829
ATATGCT453.9881925E-628.77777935
TACGGCC453.9881925E-628.77777919
CCGTTAT453.9881925E-628.7777799
ATTGTTA358.845663E-426.4285714
TTGTTAC358.845663E-426.4285715
TTATTGT358.845663E-426.4285712
AGGTCGG358.845663E-426.4285719
ATGCCCC856.91216E-1126.11764737
GACCGTT509.045902E-625.8999987
Produced by FastQC (version 0.11.5)