FastQCFastQC Report
Fri 10 Feb 2017
ERR1631366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527847
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.1729667877244732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7400.1401921390099783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7020.13299308322297937No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6350.12030001117748135No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT5770.10931197866048306No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA5420.10268126938298408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTT358.8630145E-426.4285721
GGATAGA451.3218881E-424.6666661
GGTATCA3450.021.4492741
GCAGTCG806.945447E-720.81259
TCTAGAC1600.019.656253
AGTCGGT851.242699E-619.58823611
CTAGATA1306.9303496E-1018.53
GTTCTAG1700.018.51
CCGATTT609.227795E-418.520
GTCTAGA1501.2732926E-1118.51
TCCGATT500.007029966518.49999819
TATGGGG500.007029966518.4999985
TAGAACT1653.6379788E-1217.9393964
CCGATCG1351.1477823E-917.81481418
TCTATGG1351.1477823E-917.8148142
CTCTATG1258.547431E-917.761
GCGTTAT1156.3853804E-817.6956521
CGTTATT1156.3853804E-817.6956522
TTGCGCG1054.7851245E-717.61904718
CTAGACA1900.017.5263164
Produced by FastQC (version 0.11.5)