##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631366.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527847
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06345020432057	34.0	33.0	34.0	31.0	34.0
2	33.200558116272326	34.0	33.0	34.0	31.0	34.0
3	33.29019772775065	34.0	34.0	34.0	31.0	34.0
4	36.50800705507467	37.0	37.0	37.0	35.0	37.0
5	36.49408446008029	37.0	37.0	37.0	35.0	37.0
6	36.53966016667709	37.0	37.0	37.0	35.0	37.0
7	36.557582026609985	37.0	37.0	37.0	35.0	37.0
8	36.55372295381048	37.0	37.0	37.0	35.0	37.0
9	38.411342680738926	39.0	39.0	39.0	37.0	39.0
10	38.374500565504775	39.0	39.0	39.0	37.0	39.0
11	38.42651563805421	39.0	39.0	39.0	37.0	39.0
12	38.3918256616027	39.0	39.0	39.0	37.0	39.0
13	38.40986308532586	39.0	39.0	39.0	37.0	39.0
14	39.96573817791898	41.0	40.0	41.0	38.0	41.0
15	39.95567276123574	41.0	40.0	41.0	38.0	41.0
16	39.92663593806539	41.0	40.0	41.0	38.0	41.0
17	39.929083617032965	41.0	40.0	41.0	38.0	41.0
18	39.90136914674139	41.0	40.0	41.0	38.0	41.0
19	39.91145350830828	41.0	40.0	41.0	38.0	41.0
20	39.87386685914669	41.0	40.0	41.0	38.0	41.0
21	39.85416796912742	41.0	40.0	41.0	38.0	41.0
22	39.79722722682899	41.0	40.0	41.0	38.0	41.0
23	39.75754148455897	41.0	40.0	41.0	38.0	41.0
24	39.72342553808206	41.0	40.0	41.0	37.0	41.0
25	39.72606645486287	41.0	40.0	41.0	37.0	41.0
26	39.643929017309944	41.0	40.0	41.0	37.0	41.0
27	39.60124808893486	41.0	40.0	41.0	37.0	41.0
28	39.54392844896343	41.0	40.0	41.0	37.0	41.0
29	39.504286279925815	41.0	40.0	41.0	37.0	41.0
30	39.44935369529428	41.0	39.0	41.0	36.0	41.0
31	39.41183335322546	41.0	39.0	41.0	36.0	41.0
32	39.343840165805624	41.0	39.0	41.0	36.0	41.0
33	39.26519616479776	41.0	39.0	41.0	35.0	41.0
34	39.22375044283665	41.0	39.0	41.0	35.0	41.0
35	39.1395631688728	41.0	39.0	41.0	35.0	41.0
36	39.08649476079243	41.0	39.0	41.0	35.0	41.0
37	39.03170615727664	41.0	39.0	41.0	35.0	41.0
38	38.96532328496705	41.0	39.0	41.0	35.0	41.0
39	38.89512680757871	40.0	39.0	41.0	35.0	41.0
40	38.81139610531082	40.0	38.0	41.0	35.0	41.0
41	38.73726856456511	40.0	38.0	41.0	35.0	41.0
42	38.616277065134405	40.0	38.0	41.0	35.0	41.0
43	37.8517979641828	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	3.0
16	2.0
17	3.0
18	10.0
19	14.0
20	32.0
21	57.0
22	79.0
23	155.0
24	291.0
25	400.0
26	624.0
27	896.0
28	1335.0
29	1744.0
30	2389.0
31	3314.0
32	4293.0
33	5758.0
34	8540.0
35	12632.0
36	20733.0
37	39403.0
38	97094.0
39	328045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.733506110672224	17.4154631929328	11.783338732625174	27.067691963769803
2	19.391982904136995	19.637129698567957	33.36629743088433	27.60458996641072
3	20.161902975672874	20.56864962763831	28.597301869670567	30.672145527018245
4	14.77265192375821	14.77909318419921	33.01221755546588	37.4360373365767
5	16.83480250906039	34.28095641350619	31.901668475903055	16.982572601530368
6	36.00588806983842	33.405323891203324	15.22789747786764	15.360890561090617
7	31.498142454158117	28.942098752100513	19.74890451210294	19.81085428163843
8	28.503903593276082	31.394513940592635	19.430441017946485	20.671141448184795
9	27.347318446443765	13.904407906078845	18.907941126879567	39.840332520597826
10	18.817384583032585	24.944538853114633	30.582157329680758	25.655919234172025
11	38.17526669659958	20.560124429995813	20.13916911529288	21.125439758111725
12	23.843462215376803	24.402904629561217	26.89169399466133	24.861939160400645
13	31.273835031742152	17.86085740754423	24.226527762779746	26.63877979793387
14	24.114563500408263	19.215984935028523	23.183043571337908	33.486407993225306
15	26.96160061532982	25.8084255475545	20.78045342684528	26.4495204102704
16	26.837890525095343	24.37335061106722	22.45745452754302	26.33130433629442
17	25.795732475509002	24.746375370135667	23.388595558940377	26.069296595414958
18	26.144128885832448	22.97938607210044	24.656955519307676	26.219529522759437
19	26.06759155588646	23.73547637857182	24.035373886751273	26.161558178790443
20	26.290762285283424	23.29102940814289	24.06739074011977	26.350817566453916
21	27.634712331414217	23.60475668138684	23.906548677931294	24.853982309267646
22	27.61368351056272	23.014813004525934	23.27663129656889	26.094872188342457
23	26.28204763880443	23.27833633609739	24.032153256530776	26.40746276856741
24	25.753864282642507	23.764840948229317	23.815234338738307	26.666060430389866
25	26.368057410575414	23.01632859521793	24.04996144716177	26.565652547044884
26	26.398937570924907	23.765409294738816	23.905980331421794	25.92967280291448
27	26.39079127095541	23.021633162639933	23.947848524288286	26.639727042116373
28	25.69437734798152	23.200283415459406	24.62455976826618	26.480779468292898
29	25.30297605177258	23.61744975343234	24.46182321771271	26.617750977082373
30	24.72856717950467	24.253050599889743	24.995500590133126	26.022881630472465
31	26.347975833906418	23.630332274314338	23.394089575198873	26.627602316580372
32	25.526146781169544	23.306564212735886	23.98024427532978	27.187044730764786
33	25.072037920079115	22.938654572252943	25.10291808042861	26.886389427239333
34	26.26329220399093	22.368224125551535	24.877284516157143	26.491199154300393
35	25.574835132150035	22.5929104456405	25.754811526825005	26.077442895384458
36	24.928056804339136	22.591963201458	25.733214359464014	26.746765634738857
37	25.297292586677578	21.905968964491603	25.86526019850449	26.931478250326325
38	24.346827773957227	21.40487679194918	27.131536221670295	27.116759212423297
39	24.841099788385648	21.207660553152714	27.265097651402776	26.686142007058866
40	23.849714026981303	22.089734335896576	27.80597407961019	26.254577557511933
41	23.722025511180323	21.528965779856662	28.437596500501094	26.31141220846192
42	23.8730162338708	21.923398257449602	28.3765939751481	25.826991533531498
43	22.265542856168548	21.320003713197195	28.555623125640572	27.85883030499368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	69.5
2	65.0
3	136.0
4	207.0
5	207.0
6	239.5
7	272.0
8	251.5
9	231.0
10	354.5
11	478.0
12	478.0
13	732.5
14	987.0
15	1433.5
16	1880.0
17	1804.5
18	1729.0
19	1729.0
20	1907.5
21	2086.0
22	1979.5
23	1873.0
24	2164.5
25	2456.0
26	2456.0
27	3029.0
28	3602.0
29	4536.5
30	5471.0
31	6553.0
32	7635.0
33	7635.0
34	9784.5
35	11934.0
36	14464.0
37	16994.0
38	19459.0
39	21924.0
40	21924.0
41	24073.5
42	26223.0
43	28640.5
44	31058.0
45	33991.5
46	36925.0
47	36925.0
48	38486.5
49	40048.0
50	41327.5
51	42607.0
52	43161.0
53	43715.0
54	43715.0
55	42040.5
56	40366.0
57	39055.0
58	37744.0
59	36749.5
60	35755.0
61	35755.0
62	33047.0
63	30339.0
64	27923.0
65	25507.0
66	22761.0
67	20015.0
68	20015.0
69	16787.0
70	13559.0
71	11607.5
72	9656.0
73	7406.0
74	5156.0
75	5156.0
76	4106.5
77	3057.0
78	2535.0
79	2013.0
80	1761.0
81	1509.0
82	1509.0
83	1271.0
84	1033.0
85	901.0
86	769.0
87	682.0
88	595.0
89	595.0
90	420.0
91	245.0
92	139.0
93	33.0
94	25.5
95	18.0
96	18.0
97	10.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	527847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19369069009963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.96686854161771	51.47889916739074
2	6.606309605529348	7.8210369478547666
3	2.2138135439350766	3.931313824957386
4	1.1059689141863112	2.618655272768394
5	0.6559761784796743	1.9414825504499715
6	0.4380544948894562	1.555803736553658
7	0.2983341951722979	1.2361651449916171
8	0.2205188002365091	1.044265732204143
9	0.1605497588245858	0.8553179487809364
>10	1.1159306556590027	12.793895435305751
>50	0.13407770329650662	5.575952637965712
>100	0.08134690333812601	8.265816354668916
>500	0.0022507048354422207	0.8813952461080299
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.1729667877244732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	740	0.1401921390099783	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	702	0.13299308322297937	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	635	0.12030001117748135	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	577	0.10931197866048306	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	542	0.10268126938298408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
6	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
7	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
8	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
9	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
10	0.0	1.8944883649997063E-4	0.0	0.0	1.8944883649997063E-4
11	0.0	1.8944883649997063E-4	0.0	1.8944883649997063E-4	1.8944883649997063E-4
12	0.0	1.8944883649997063E-4	0.0	7.577953459998825E-4	1.8944883649997063E-4
13	0.0	1.8944883649997063E-4	0.0	7.577953459998825E-4	1.8944883649997063E-4
14	0.0	1.8944883649997063E-4	0.0	7.577953459998825E-4	1.8944883649997063E-4
15	0.0	1.8944883649997063E-4	0.0	0.0013261418554997944	1.8944883649997063E-4
16	0.0	1.8944883649997063E-4	0.0	0.0017050395284997357	1.8944883649997063E-4
17	0.0	1.8944883649997063E-4	0.0	0.0018944883649997064	1.8944883649997063E-4
18	0.0	1.8944883649997063E-4	0.0	0.0018944883649997064	1.8944883649997063E-4
19	0.0	1.8944883649997063E-4	0.0	0.0018944883649997064	1.8944883649997063E-4
20	0.0	1.8944883649997063E-4	0.0	0.0022733860379996478	1.8944883649997063E-4
21	0.0	1.8944883649997063E-4	0.0	0.0022733860379996478	1.8944883649997063E-4
22	0.0	1.8944883649997063E-4	0.0	0.0034100790569994714	1.8944883649997063E-4
23	0.0	1.8944883649997063E-4	0.0	0.004167874402999354	1.8944883649997063E-4
24	0.0	1.8944883649997063E-4	0.0	0.007956851132998767	1.8944883649997063E-4
25	0.0	1.8944883649997063E-4	0.0	0.011177481353498267	1.8944883649997063E-4
26	0.0	1.8944883649997063E-4	0.0	0.016671497611997415	1.8944883649997063E-4
27	0.0	1.8944883649997063E-4	0.0	0.05816079280549098	1.8944883649997063E-4
28	0.0	1.8944883649997063E-4	0.0	0.22677025729046485	1.8944883649997063E-4
29	0.0	1.8944883649997063E-4	0.0	0.4484253959954305	1.8944883649997063E-4
30	0.0	1.8944883649997063E-4	0.0	0.7428288879163849	1.8944883649997063E-4
31	0.0	1.8944883649997063E-4	0.0	1.4638711596352731	3.7889767299994126E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTT	35	8.8630145E-4	26.42857	21
GGATAGA	45	1.3218881E-4	24.666666	1
GGTATCA	345	0.0	21.449274	1
GCAGTCG	80	6.945447E-7	20.8125	9
TCTAGAC	160	0.0	19.65625	3
AGTCGGT	85	1.242699E-6	19.588236	11
CTAGATA	130	6.9303496E-10	18.5	3
GTTCTAG	170	0.0	18.5	1
CCGATTT	60	9.227795E-4	18.5	20
GTCTAGA	150	1.2732926E-11	18.5	1
TCCGATT	50	0.0070299665	18.499998	19
TATGGGG	50	0.0070299665	18.499998	5
TAGAACT	165	3.6379788E-12	17.939396	4
CCGATCG	135	1.1477823E-9	17.814814	18
TCTATGG	135	1.1477823E-9	17.814814	2
CTCTATG	125	8.547431E-9	17.76	1
GCGTTAT	115	6.3853804E-8	17.695652	1
CGTTATT	115	6.3853804E-8	17.695652	2
TTGCGCG	105	4.7851245E-7	17.619047	18
CTAGACA	190	0.0	17.526316	4
>>END_MODULE