##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5899
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12578403119173	33.0	31.0	34.0	30.0	34.0
2	32.292761484997456	34.0	31.0	34.0	30.0	34.0
3	32.405661976606204	34.0	31.0	34.0	30.0	34.0
4	35.83929479572809	37.0	35.0	37.0	35.0	37.0
5	35.8409899983048	37.0	35.0	37.0	35.0	37.0
6	35.879301576538396	37.0	35.0	37.0	35.0	37.0
7	35.90337345312765	37.0	35.0	37.0	35.0	37.0
8	35.85963722664859	37.0	35.0	37.0	35.0	37.0
9	37.53704017630107	39.0	37.0	39.0	35.0	39.0
10	37.518901508730295	39.0	37.0	39.0	35.0	39.0
11	37.626038311578235	39.0	37.0	39.0	35.0	39.0
12	37.56568909984743	39.0	37.0	39.0	35.0	39.0
13	37.59620274622817	39.0	37.0	39.0	35.0	39.0
14	38.86099338870995	40.0	38.0	41.0	35.0	41.0
15	38.87184268520088	40.0	38.0	41.0	35.0	41.0
16	38.74758433632819	40.0	38.0	41.0	35.0	41.0
17	38.838108153924395	40.0	38.0	41.0	35.0	41.0
18	38.83844719443974	40.0	38.0	41.0	35.0	41.0
19	38.78776063739617	40.0	38.0	41.0	34.0	41.0
20	38.802508899813525	40.0	38.0	41.0	35.0	41.0
21	38.80352602135955	40.0	38.0	41.0	34.0	41.0
22	38.621461264621125	40.0	38.0	41.0	34.0	41.0
23	38.601118833700625	40.0	38.0	41.0	34.0	41.0
24	38.567045261908795	40.0	38.0	41.0	34.0	41.0
25	38.4137989489744	40.0	38.0	41.0	34.0	41.0
26	38.385997626716396	40.0	38.0	41.0	34.0	41.0
27	38.2547889472792	40.0	38.0	41.0	34.0	41.0
28	38.084421088320056	40.0	38.0	41.0	33.0	41.0
29	38.03186980844211	40.0	37.0	41.0	33.0	41.0
30	37.89777928462451	40.0	37.0	41.0	33.0	41.0
31	37.754534666892695	40.0	37.0	41.0	33.0	41.0
32	37.589760976436686	40.0	37.0	41.0	33.0	41.0
33	37.48669265977284	40.0	36.0	41.0	32.0	41.0
34	37.403627733514156	40.0	36.0	41.0	32.0	41.0
35	37.31039159179522	40.0	35.0	41.0	32.0	41.0
36	37.1712154602475	40.0	35.0	41.0	31.0	41.0
37	37.08798101373114	40.0	35.0	41.0	31.0	41.0
38	36.8438718426852	40.0	35.0	41.0	31.0	41.0
39	36.750635700966264	39.0	35.0	41.0	31.0	41.0
40	36.56518053907442	39.0	35.0	41.0	30.0	41.0
41	36.449228682827595	39.0	35.0	41.0	30.0	41.0
42	36.21681640956094	39.0	35.0	41.0	29.0	41.0
43	35.367011357857265	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	3.0
22	5.0
23	9.0
24	13.0
25	27.0
26	32.0
27	38.0
28	57.0
29	71.0
30	103.0
31	132.0
32	149.0
33	184.0
34	230.0
35	300.0
36	438.0
37	703.0
38	1148.0
39	2251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.041023902356336	18.392947957280896	14.070181386675706	25.49584675368707
2	19.054076962196984	21.444312595355143	33.90405153415833	25.597558908289543
3	20.596711307001186	22.15629767757247	29.71690116968978	27.530089845736565
4	13.917613154771995	16.935073741312088	33.49720291574843	35.65011018816749
5	15.172063061535853	36.37904729615189	31.835904390574676	16.612985251737584
6	32.32751313781997	34.75165282251229	16.64688930327174	16.273944736395997
7	29.055772164773693	30.496694354975418	20.56280725546703	19.88472622478386
8	30.140701813866755	30.903542973385317	19.90167825055094	19.054076962196984
9	28.3946431598576	13.646380742498728	19.833870147482624	38.12510595016104
10	18.952364807594506	26.81810476351924	29.564332937786062	24.665197491100184
11	35.853534497372436	21.766401084929647	19.91863027631802	22.461434141379897
12	22.749618579420243	27.39447363959993	27.852178335311066	22.003729445668757
13	30.88659094761824	18.7150364468554	25.733175114426178	24.665197491100184
14	23.478555687404643	20.850991693507375	25.088998135277162	30.581454483810816
15	26.682488557382605	27.004577046957113	21.986777419901678	24.326156975758604
16	24.868621800305135	26.072215629767758	24.309204949991525	24.749957619935582
17	23.020850991693507	26.97067299542295	25.512798779454144	24.495677233429394
18	23.851500254280385	23.800644176979148	27.46228174266825	24.885573826072218
19	23.868452280047467	25.444990676385824	27.852178335311066	22.834378708255638
20	23.76674012544499	24.461773181895236	27.530089845736565	24.24139684692321
21	27.563993897270723	23.42769961010341	26.02135955246652	22.98694694015935
22	25.953551449398205	24.427869130361078	25.885743346329885	23.732836073910832
23	23.20732327513138	24.682149516867266	27.716562129174438	24.39396507882692
24	23.020850991693507	25.072046109510087	27.445329716901167	24.461773181895236
25	23.054755043227665	25.17375826411256	27.30971351076454	24.461773181895236
26	24.427869130361078	24.461773181895236	27.445329716901167	23.665027970842516
27	23.732836073910832	24.59738938803187	26.885912866587557	24.78386167146974
28	22.800474656721477	24.37701305305984	28.530259365994237	24.292252924224446
29	21.834209187997967	25.394134599084587	28.3946431598576	24.37701305305984
30	22.12239362603831	25.868791320562806	28.445499237158838	23.563315816240042
31	23.546363790472963	25.902695372096968	26.733344634683842	23.817596202746227
32	22.851330734022714	25.038142057975925	27.936938464146465	24.17358874385489
33	21.613832853025936	24.851669774538056	28.750635700966264	24.78386167146974
34	23.088659094761823	25.49584675368707	27.22495338192914	24.190540769621972
35	23.52941176470588	25.42803865061875	27.47923376843533	23.563315816240042
36	22.071537548737076	25.42803865061875	27.30971351076454	25.190710289879643
37	22.274961857942024	24.037972537718257	28.835395829801662	24.851669774538056
38	22.512290218681134	23.512459738938805	28.699779623665027	25.275470418715035
39	22.32581793524326	23.495507713171722	29.6151890150873	24.563485336497713
40	21.986777419901678	22.919138837091033	31.157823359891506	23.93626038311578
41	20.834039667740296	23.275131378199696	31.598576029835566	24.292252924224446
42	21.105272080013563	24.037972537718257	30.88659094761824	23.97016443464994
43	19.986438379386335	23.83454822851331	30.835734870317005	25.343278521783354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	4.0
4	8.0
5	8.0
6	11.5
7	15.0
8	14.0
9	13.0
10	21.5
11	30.0
12	30.0
13	47.0
14	64.0
15	89.0
16	114.0
17	103.0
18	92.0
19	92.0
20	99.0
21	106.0
22	81.5
23	57.0
24	52.5
25	48.0
26	48.0
27	56.0
28	64.0
29	75.5
30	87.0
31	107.5
32	128.0
33	128.0
34	154.5
35	181.0
36	201.0
37	221.0
38	235.0
39	249.0
40	249.0
41	280.0
42	311.0
43	337.0
44	363.0
45	375.0
46	387.0
47	387.0
48	405.5
49	424.0
50	441.5
51	459.0
52	430.5
53	402.0
54	402.0
55	393.5
56	385.0
57	355.0
58	325.0
59	308.5
60	292.0
61	292.0
62	292.0
63	292.0
64	257.0
65	222.0
66	195.5
67	169.0
68	169.0
69	155.0
70	141.0
71	120.0
72	99.0
73	77.5
74	56.0
75	56.0
76	43.5
77	31.0
78	29.0
79	27.0
80	21.0
81	15.0
82	15.0
83	11.5
84	8.0
85	6.0
86	4.0
87	5.5
88	7.0
89	7.0
90	3.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.08289540600101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.51950523311132	84.20071198508222
2	3.387250237868696	6.034921173080183
3	1.027592768791627	2.746228174266825
4	0.5899143672692674	2.1020511951178165
5	0.20932445290199808	0.9323614171893542
6	0.07611798287345385	0.40684861840989994
7	0.019029495718363463	0.11866418036955416
8	0.038058991436726926	0.27123241227326667
9	0.038058991436726926	0.305136463807425
>10	0.07611798287345385	1.949482963214104
>50	0.019029495718363463	0.9323614171893542
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.9323614171893542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.8136972368197999	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.6950330564502458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.25428038650618745	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.18647228343787084	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1525682319037125	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	9	0.1525682319037125	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.13561620613663333	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	8	0.13561620613663333	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	7	0.11866418036955416	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	6	0.10171215460247499	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	6	0.10171215460247499	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	6	0.10171215460247499	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	6	0.10171215460247499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.016952025767079167	0.0
20	0.0	0.0	0.0	0.016952025767079167	0.0
21	0.0	0.0	0.0	0.016952025767079167	0.0
22	0.0	0.0	0.0	0.016952025767079167	0.0
23	0.0	0.0	0.0	0.0508560773012375	0.0
24	0.0	0.0	0.0	0.06780810306831667	0.0
25	0.0	0.0	0.0	0.08476012883539583	0.0
26	0.0	0.0	0.0	0.101712154602475	0.0
27	0.0	0.0	0.0	0.11866418036955416	0.0
28	0.0	0.0	0.0	0.20342430920495	0.0
29	0.0	0.0	0.0	0.35599254110866246	0.0
30	0.0	0.0	0.0	0.6611290049160875	0.0
31	0.0	0.0	0.0	1.2883539582980166	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE