FastQCFastQC Report
Fri 10 Feb 2017
ERR1631364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186636
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.17788636704601468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2610.13984440300906578No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2490.133414775284511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2160.11573329904198547No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2050.10983947362781028No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2040.10930367131743073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG250.005484698329.637
GTCCAGA400.001925163223.1250021
ACCTCTA502.6903662E-422.225
AAGCATT603.7047987E-521.58333422
AGGAGTG705.064232E-621.1428595
ACATGCA450.00381398820.55555727
ATACACT450.00381398820.5555574
CAGACCG450.00381398820.5555575
CGAAAGC555.120939E-420.18181819
AGCATTT555.120939E-420.18181823
GAGCGAA555.120939E-420.18181816
AAAGCAT555.120939E-420.18181821
CTTATAC3450.019.8405837
GGTATCA2150.019.7906991
CGACGGT500.00701335818.518
GCGAAAG609.197348E-418.518
GGGTAAT609.197348E-418.511
CGACCCG609.197348E-418.55
TCTAGCG609.197348E-418.528
GTAATTT701.21226636E-418.513
Produced by FastQC (version 0.11.5)