##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186636
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97728734006301	34.0	33.0	34.0	31.0	34.0
2	33.12441329647014	34.0	33.0	34.0	31.0	34.0
3	33.20733942004758	34.0	33.0	34.0	31.0	34.0
4	36.45439250734049	37.0	37.0	37.0	35.0	37.0
5	36.42417861505819	37.0	37.0	37.0	35.0	37.0
6	36.47506911849804	37.0	37.0	37.0	35.0	37.0
7	36.484751066246595	37.0	37.0	37.0	35.0	37.0
8	36.481589832615356	37.0	37.0	37.0	35.0	37.0
9	38.31838444887374	39.0	39.0	39.0	37.0	39.0
10	38.269813969437834	39.0	39.0	39.0	37.0	39.0
11	38.32611071818942	39.0	39.0	39.0	37.0	39.0
12	38.284227051587045	39.0	39.0	39.0	37.0	39.0
13	38.320597312415615	39.0	39.0	39.0	37.0	39.0
14	39.83903426991577	41.0	40.0	41.0	38.0	41.0
15	39.81962215221072	41.0	40.0	41.0	38.0	41.0
16	39.77704194260485	41.0	40.0	41.0	38.0	41.0
17	39.77360209177222	41.0	40.0	41.0	38.0	41.0
18	39.73352407895583	41.0	40.0	41.0	38.0	41.0
19	39.737917657900944	41.0	40.0	41.0	38.0	41.0
20	39.70982554276774	41.0	40.0	41.0	38.0	41.0
21	39.67403930645749	41.0	40.0	41.0	37.0	41.0
22	39.60277759917701	41.0	40.0	41.0	37.0	41.0
23	39.541219271737496	41.0	40.0	41.0	37.0	41.0
24	39.50461861591547	41.0	40.0	41.0	37.0	41.0
25	39.5139737242547	41.0	40.0	41.0	37.0	41.0
26	39.40024968387664	41.0	39.0	41.0	36.0	41.0
27	39.35976446130436	41.0	39.0	41.0	36.0	41.0
28	39.27986026275745	41.0	39.0	41.0	36.0	41.0
29	39.23830343556441	41.0	39.0	41.0	36.0	41.0
30	39.1775863177522	41.0	39.0	41.0	35.0	41.0
31	39.12148781585546	41.0	39.0	41.0	35.0	41.0
32	39.06503032641077	41.0	39.0	41.0	35.0	41.0
33	38.99043057073662	41.0	39.0	41.0	35.0	41.0
34	38.93691999399901	40.0	39.0	41.0	35.0	41.0
35	38.83001135900898	40.0	38.0	41.0	35.0	41.0
36	38.775723868921325	40.0	38.0	41.0	35.0	41.0
37	38.70834672839109	40.0	38.0	41.0	35.0	41.0
38	38.63935146488352	40.0	38.0	41.0	35.0	41.0
39	38.56358901819585	40.0	38.0	41.0	35.0	41.0
40	38.45449430977946	40.0	38.0	41.0	35.0	41.0
41	38.36058423883924	40.0	38.0	41.0	35.0	41.0
42	38.232120276902634	40.0	37.0	41.0	34.0	41.0
43	37.40876358258857	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	5.0
20	16.0
21	27.0
22	47.0
23	87.0
24	134.0
25	204.0
26	261.0
27	423.0
28	608.0
29	778.0
30	1134.0
31	1409.0
32	1853.0
33	2463.0
34	3777.0
35	5193.0
36	8634.0
37	16204.0
38	38739.0
39	104636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03296255813455	18.050644034377076	12.135386527786709	26.781006879701664
2	20.169206369617864	19.48605842388392	32.91594333354765	27.42879187295056
3	20.662680297477444	20.955764161255065	28.385198996978072	29.99635654428942
4	15.517906513212884	15.470220107589103	32.34424226837266	36.66763111082535
5	17.32677511305429	33.79198011101824	31.46177586317752	17.419468912749952
6	35.88535973767119	32.70215821170621	15.54683983797338	15.86564221264922
7	30.81399086992863	28.751151974967314	20.095265650785485	20.339591504318566
8	28.393771833944147	30.856319252448618	19.411046100430784	21.33886281317645
9	27.36824621187767	13.972116847767849	19.28352515056045	39.37611178979404
10	19.336569579288028	24.308279217299987	30.1147688548833	26.240382348528684
11	38.42720589811183	20.47300627960308	19.816648449388115	21.283139372896976
12	23.416168370518015	24.086992863113228	26.87530808632847	25.621530680040294
13	31.416768469105637	17.645577487730126	23.808911464026234	27.128742579138
14	24.133607664116248	19.802181787007868	22.868042606999722	33.19616794187616
15	27.197861077176967	25.93979725240575	20.3465569343535	26.51578473606378
16	27.185001821727855	24.261664416296963	22.421719282453545	26.131614479521637
17	25.937118240853856	24.67101738142695	23.40652392893118	25.985340448788012
18	26.149295955764163	22.753380912578493	24.933560513512933	26.16376261814441
19	26.156797188109476	24.068239782249943	24.190938511326863	25.584024518313726
20	26.445594633404056	23.116654879015837	23.815876894061166	26.621873593518934
21	27.985490473434922	23.365267151031954	23.424741207484086	25.224501168049034
22	27.643112797102383	23.0127092308022	23.40920294048308	25.934975031612336
23	26.21734284918237	22.887331490173384	24.015731155832746	26.879594504811504
24	25.837994813433635	24.09770890932082	23.717825071261707	26.34647120598384
25	26.437557598748366	23.045928974045736	23.64281274780857	26.873700679397327
26	26.07964165541482	24.246661951606335	24.030733620523375	25.642962772455473
27	26.7172464047665	22.937161105038683	23.747294198332582	26.598298291862232
28	25.507404787929445	23.459568357658757	24.665659358323154	26.367367496088644
29	25.275402387535095	23.635311515463258	24.876229666302322	26.21305643069933
30	24.779785250434	24.25148417239975	24.860155596990936	26.108574980175312
31	26.325574915879034	23.693713967294627	23.416168370518015	26.564542746308323
32	25.105553055144775	23.02824749780321	24.310422426541503	27.555777020510515
33	24.728884030947942	22.816605585203284	24.98606913993013	27.468441243918644
34	26.035705865963692	22.60014145180994	24.886409910199532	26.477742772026836
35	25.975696007201183	22.226687241475386	25.760839280738978	26.036777470584454
36	24.47169892196575	22.847146316894918	25.556162798173986	27.124991962965346
37	24.933560513512933	21.676418268715576	26.458453888853168	26.93156732891832
38	24.06984718918108	21.02273945005251	27.603463426134294	27.303949934632115
39	24.048415096765897	20.73769262093058	27.86868556977218	27.345206712531343
40	23.44617329989927	21.56014916736321	28.6230952227866	26.37058230995092
41	22.61192910263829	21.503889924773357	29.56610728905463	26.318073683533722
42	23.23935360809276	21.832336741036027	29.001907456224952	25.926402194646265
43	21.751430592168713	21.23813197882509	29.552712231295143	27.457725197711053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	23.0
2	22.0
3	55.0
4	88.0
5	88.0
6	92.0
7	96.0
8	85.0
9	74.0
10	117.0
11	160.0
12	160.0
13	286.5
14	413.0
15	595.5
16	778.0
17	714.0
18	650.0
19	650.0
20	786.0
21	922.0
22	871.5
23	821.0
24	981.5
25	1142.0
26	1142.0
27	1331.0
28	1520.0
29	1841.5
30	2163.0
31	2582.5
32	3002.0
33	3002.0
34	3745.0
35	4488.0
36	5411.0
37	6334.0
38	7204.5
39	8075.0
40	8075.0
41	8731.5
42	9388.0
43	10090.5
44	10793.0
45	11366.5
46	11940.0
47	11940.0
48	12720.5
49	13501.0
50	13772.0
51	14043.0
52	14395.0
53	14747.0
54	14747.0
55	14273.5
56	13800.0
57	13396.0
58	12992.0
59	12601.5
60	12211.0
61	12211.0
62	11445.0
63	10679.0
64	9898.0
65	9117.0
66	8118.5
67	7120.0
68	7120.0
69	6103.5
70	5087.0
71	4501.5
72	3916.0
73	3061.5
74	2207.0
75	2207.0
76	1808.5
77	1410.0
78	1151.0
79	892.0
80	811.5
81	731.0
82	731.0
83	610.0
84	489.0
85	430.5
86	372.0
87	331.5
88	291.0
89	291.0
90	203.5
91	116.0
92	64.5
93	13.0
94	9.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	186636.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.44137777188404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49849831135546	57.029651619264975
2	6.02190961953009	7.76120305400562
3	1.8238112434691305	3.525867279750114
4	0.898992679013559	2.317293073698833
5	0.49341609842803336	1.5898206598765008
6	0.3695111561972562	1.428708480445982
7	0.2808097074563656	1.2667035108145526
8	0.20711099723817605	1.0677214410989546
9	0.16130540083027162	0.9355268044393837
>10	1.085321558198413	13.994338591430514
>50	0.11749350585891025	5.206971454661739
>100	0.04181972242435789	3.8761940305128237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.17788636704601468	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	261	0.13984440300906578	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	249	0.133414775284511	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.11573329904198547	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	205	0.10983947362781028	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	204	0.10930367131743073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	5.358023103795624E-4	0.0
14	0.0	0.0	0.0	5.358023103795624E-4	0.0
15	0.0	0.0	0.0	0.0010716046207591247	0.0
16	0.0	0.0	0.0	0.0010716046207591247	0.0
17	0.0	0.0	0.0	0.0010716046207591247	0.0
18	0.0	0.0	0.0	0.0010716046207591247	0.0
19	0.0	0.0	0.0	0.0010716046207591247	0.0
20	0.0	0.0	0.0	0.001607406931138687	0.0
21	0.0	0.0	0.0	0.002679011551897812	0.0
22	0.0	0.0	0.0	0.003214813862277374	0.0
23	0.0	0.0	0.0	0.006429627724554748	0.0
24	0.0	0.0	0.0	0.010180243897211685	0.0
25	0.0	0.0	0.0	0.016609871621766432	0.0
26	0.0	0.0	0.0	0.025718510898218992	0.0
27	0.0	0.0	0.0	0.09751602048908035	0.0
28	0.0	0.0	0.0	0.3016567007436936	0.0
29	0.0	0.0	0.0	0.5599134143466427	0.0
30	0.0	0.0	0.0	0.8787157890224823	0.0
31	0.0	0.0	0.0	1.671167406073855	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGATAG	25	0.0054846983	29.6	37
GTCCAGA	40	0.0019251632	23.125002	1
ACCTCTA	50	2.6903662E-4	22.2	25
AAGCATT	60	3.7047987E-5	21.583334	22
AGGAGTG	70	5.064232E-6	21.142859	5
ACATGCA	45	0.003813988	20.555557	27
ATACACT	45	0.003813988	20.555557	4
CAGACCG	45	0.003813988	20.555557	5
CGAAAGC	55	5.120939E-4	20.181818	19
AGCATTT	55	5.120939E-4	20.181818	23
GAGCGAA	55	5.120939E-4	20.181818	16
AAAGCAT	55	5.120939E-4	20.181818	21
CTTATAC	345	0.0	19.84058	37
GGTATCA	215	0.0	19.790699	1
CGACGGT	50	0.007013358	18.5	18
GCGAAAG	60	9.197348E-4	18.5	18
GGGTAAT	60	9.197348E-4	18.5	11
CGACCCG	60	9.197348E-4	18.5	5
TCTAGCG	60	9.197348E-4	18.5	28
GTAATTT	70	1.21226636E-4	18.5	13
>>END_MODULE