FastQCFastQC Report
Fri 10 Feb 2017
ERR1631363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223860
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.47521775366463487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49070.40094455248149297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40880.33402513359371167No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC31910.26073243671661794No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT23380.19103492229503374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.1626002974196395No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18570.15173304136093996No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC14870.12150082525779092No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14330.11708855588057458No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14110.11529096465281977No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG13900.11357508211723563No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG13210.1079371823574592No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG13150.10744693020443516No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT13040.10654813459055774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT12660.10344320428807216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA1005.347829E-1022.21
GCAGTCG7900.019.2025329
CAGTCGG7950.019.0817610
AGTCGGT8050.019.07453511
TCCCGTA500.00703513518.52
GGACCGT500.00703513518.56
CGGTGAT8500.017.8470614
GTGATTC8500.017.62941216
GGCAGTC8650.017.537578
ATACACA5600.017.5089337
CGGTCCA3500.017.44285810
TTCTAGC852.7233482E-517.4117642
GGTATCA27500.017.3563631
CTCTATG13650.017.2124541
TGCATCA7450.017.13422814
CAGGACA8800.017.0284084
TCGGTGA8800.017.02840813
CCGCTTA2950.016.93220125
GTCGGTG8750.016.91428612
GTACTAT1655.4569682E-1116.818181
Produced by FastQC (version 0.11.5)