##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1223860
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.031826352687396	34.0	33.0	34.0	31.0	34.0
2	33.17433039726766	34.0	33.0	34.0	31.0	34.0
3	33.24314300655304	34.0	34.0	34.0	31.0	34.0
4	36.486798326605985	37.0	37.0	37.0	35.0	37.0
5	36.47565080973314	37.0	37.0	37.0	35.0	37.0
6	36.523864657722285	37.0	37.0	37.0	35.0	37.0
7	36.53620430441391	37.0	37.0	37.0	35.0	37.0
8	36.53280358864576	37.0	37.0	37.0	35.0	37.0
9	38.37411468632033	39.0	39.0	39.0	37.0	39.0
10	38.347574068929454	39.0	39.0	39.0	37.0	39.0
11	38.393660222574475	39.0	39.0	39.0	37.0	39.0
12	38.36351461768503	39.0	39.0	39.0	37.0	39.0
13	38.38195218407334	39.0	39.0	39.0	37.0	39.0
14	39.914540878858695	41.0	40.0	41.0	38.0	41.0
15	39.90072393901263	41.0	40.0	41.0	38.0	41.0
16	39.85863170624091	41.0	40.0	41.0	38.0	41.0
17	39.85968574836991	41.0	40.0	41.0	38.0	41.0
18	39.80277074175151	41.0	40.0	41.0	38.0	41.0
19	39.81917457879169	41.0	40.0	41.0	38.0	41.0
20	39.78853708757538	41.0	40.0	41.0	38.0	41.0
21	39.76559737224846	41.0	40.0	41.0	38.0	41.0
22	39.69076119817626	41.0	40.0	41.0	37.0	41.0
23	39.630272253362314	41.0	40.0	41.0	37.0	41.0
24	39.59530828689556	41.0	40.0	41.0	37.0	41.0
25	39.59962495710294	41.0	40.0	41.0	37.0	41.0
26	39.48313450884905	41.0	40.0	41.0	36.0	41.0
27	39.432618109914536	41.0	39.0	41.0	36.0	41.0
28	39.34670305427091	41.0	39.0	41.0	35.0	41.0
29	39.28167274034612	41.0	39.0	41.0	35.0	41.0
30	39.20844622750968	41.0	39.0	41.0	35.0	41.0
31	39.155818475969475	41.0	39.0	41.0	35.0	41.0
32	39.075246351706895	41.0	39.0	41.0	35.0	41.0
33	38.97393329302371	41.0	39.0	41.0	35.0	41.0
34	38.903598450803194	41.0	39.0	41.0	35.0	41.0
35	38.77574314055529	41.0	38.0	41.0	35.0	41.0
36	38.70910725082934	40.0	38.0	41.0	35.0	41.0
37	38.626945075417126	40.0	38.0	41.0	35.0	41.0
38	38.536117693200204	40.0	38.0	41.0	35.0	41.0
39	38.43033108362068	40.0	38.0	41.0	35.0	41.0
40	38.2930849933816	40.0	38.0	41.0	35.0	41.0
41	38.169811906590624	40.0	37.0	41.0	34.0	41.0
42	38.01586374258493	40.0	37.0	41.0	34.0	41.0
43	37.18847172062164	40.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	3.0
14	2.0
15	3.0
16	6.0
17	7.0
18	21.0
19	51.0
20	84.0
21	186.0
22	303.0
23	490.0
24	790.0
25	1218.0
26	1843.0
27	2792.0
28	4025.0
29	5624.0
30	7402.0
31	9441.0
32	12107.0
33	15848.0
34	24051.0
35	37565.0
36	55700.0
37	109585.0
38	226099.0
39	708611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08036866961908	16.79505825829752	12.04655761279885	27.07801545928456
2	20.058748549670714	18.95347507067802	33.17168630398902	27.81609007566225
3	20.774189858317126	21.119817626199076	28.08826172928276	30.017730786201035
4	15.038321376628044	15.03636036801595	31.984459006749137	37.94085924860687
5	16.612357622603895	34.95081136731326	31.074714428120863	17.36211658196199
6	35.607667543673294	32.202376088768325	15.870851241155034	16.319105126403347
7	31.409720066020625	28.84521105355188	19.53009331132646	20.21497556910104
8	29.880051639893452	30.5003840308532	18.725998071674866	20.89356625757848
9	28.517395780563138	13.582517608223164	18.418855097805302	39.481231513408396
10	20.317765103851748	24.304168777474548	28.670844704459657	26.707221414214043
11	37.86740313434544	21.046116385861126	19.132417106531793	21.954063373261647
12	24.807821156014576	24.476492409262498	25.67131861487425	25.044367819848674
13	31.42263003938359	17.315215792656023	24.2120830813982	27.050071086562188
14	24.08584315199451	19.57127449218048	23.244897292173942	33.097985063651066
15	27.960060791266976	25.06054614089847	20.603500400372592	26.375892667461965
16	26.831091791544786	23.872665174121224	22.181622080956974	27.11462095337702
17	25.470070106057886	25.059892471361106	23.392299772849835	26.07773764973118
18	25.77173859755201	22.02278038337718	25.271354566698808	26.934126452372002
19	26.231921951857238	23.682120503979213	24.30188093409377	25.78407661006978
20	26.318778291634665	22.70815289330479	24.54365695422679	26.429411860833756
21	29.270341378915887	22.52087657084961	23.66463484385469	24.544147206379815
22	28.23223244488749	22.620969718758683	23.229617766738027	25.91718006961581
23	26.28666677561159	22.854248034905954	23.86400405275113	26.995081136731326
24	26.02936610396614	23.634974588596734	24.239046949814522	26.096612357622607
25	26.020950108672558	22.552579543411827	24.259964375010213	27.166505972905398
26	26.661873090059316	23.67828019544719	24.540470315232135	25.119376399261355
27	27.482064942068536	22.38434134623241	23.478175608321212	26.655418103377837
28	25.316539473469184	22.824506070955827	25.3711208798392	26.487833575735785
29	25.169708953638487	22.901393950288433	25.73170133838838	26.197195757684703
30	24.435066102331966	23.62484271076757	26.04873106401059	25.89136012288987
31	25.60962855228539	23.58382494729789	24.276224404752178	26.530322095664538
32	24.805043060480774	22.97035608648048	25.139313320150997	27.085287532887747
33	25.073537822953607	22.842155148464695	25.28655238344255	26.79775464513915
34	26.28519601915252	22.90605134574216	24.948196689163794	25.860555945941528
35	26.117284656741784	22.606752406320986	25.74657232036344	25.529390616573792
36	24.434739267563284	23.443122579379995	25.043223898158285	27.078914254898436
37	24.63704998937787	22.267252790351836	26.11450656120798	26.98119065906231
38	24.522330985570246	21.275390976092037	27.286209206935435	26.916068831402285
39	24.098426290588794	21.447632899187816	27.443171604595296	27.010769205628094
40	23.944487114539246	21.307911035575962	28.27243312143546	26.475168728449333
41	22.235059565636593	21.7845178370075	29.409409572990374	26.57101302436553
42	23.039808474825552	22.216103149052994	28.528263036621837	26.215825339499617
43	21.651741212230156	20.972905397676207	29.470936218194893	27.90441717189875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	372.0
1	300.5
2	229.0
3	563.0
4	897.0
5	897.0
6	1177.0
7	1457.0
8	1369.0
9	1281.0
10	2097.0
11	2913.0
12	2913.0
13	4655.5
14	6398.0
15	9127.0
16	11856.0
17	10412.5
18	8969.0
19	8969.0
20	10009.5
21	11050.0
22	8528.0
23	6006.0
24	6438.5
25	6871.0
26	6871.0
27	7672.0
28	8473.0
29	10213.0
30	11953.0
31	14465.0
32	16977.0
33	16977.0
34	21130.5
35	25284.0
36	30916.0
37	36548.0
38	41896.5
39	47245.0
40	47245.0
41	51044.5
42	54844.0
43	58915.0
44	62986.0
45	67794.0
46	72602.0
47	72602.0
48	78703.5
49	84805.0
50	87696.5
51	90588.0
52	93643.0
53	96698.0
54	96698.0
55	94834.0
56	92970.0
57	89015.5
58	85061.0
59	80504.0
60	75947.0
61	75947.0
62	72802.5
63	69658.0
64	64804.0
65	59950.0
66	52762.5
67	45575.0
68	45575.0
69	39670.0
70	33765.0
71	30451.0
72	27137.0
73	25401.5
74	23666.0
75	23666.0
76	21994.0
77	20322.0
78	15857.5
79	11393.0
80	7841.0
81	4289.0
82	4289.0
83	3370.0
84	2451.0
85	2250.5
86	2050.0
87	1804.0
88	1558.0
89	1558.0
90	1098.0
91	638.0
92	366.5
93	95.0
94	59.5
95	24.0
96	24.0
97	16.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1223860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.73907286362902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30775987696356	39.93375990572662
2	6.367114217571232	5.8245180225707776
3	1.932770935012783	2.6520945187576217
4	0.9579013102356035	1.7525407130012791
5	0.5867018917193704	1.3417600287290632
6	0.4070487791531216	1.1170820261241519
7	0.3001663610176891	0.9610531740458916
8	0.22453276178691214	0.8215936281314744
9	0.17517236192098562	0.721099928303816
>10	1.3616538867557317	12.70126946536819
>50	0.18270078250875776	5.952965120538532
>100	0.17355155304177486	16.766239363061164
>500	0.018626791878974737	5.7464705076767135
>1k	0.00411938666554249	3.231104534246439
>5k	1.791037680670648E-4	0.4764490637183025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5816	0.47521775366463487	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4907	0.40094455248149297	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4088	0.33402513359371167	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3191	0.26073243671661794	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2338	0.19103492229503374	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1990	0.1626002974196395	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1857	0.15173304136093996	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1487	0.12150082525779092	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1433	0.11708855588057458	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1411	0.11529096465281977	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1390	0.11357508211723563	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1321	0.1079371823574592	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1315	0.10744693020443516	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1304	0.10654813459055774	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1266	0.10344320428807216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.170869217067311E-5	0.0
3	0.0	0.0	0.0	8.170869217067311E-5	0.0
4	0.0	0.0	0.0	8.170869217067311E-5	0.0
5	0.0	0.0	0.0	8.170869217067311E-5	0.0
6	0.0	0.0	0.0	8.170869217067311E-5	8.170869217067311E-5
7	0.0	0.0	0.0	8.170869217067311E-5	8.170869217067311E-5
8	0.0	0.0	0.0	8.170869217067311E-5	8.170869217067311E-5
9	0.0	0.0	0.0	8.170869217067311E-5	8.170869217067311E-5
10	0.0	0.0	0.0	3.2683476868269244E-4	8.170869217067311E-5
11	8.170869217067311E-5	0.0	0.0	4.902521530240387E-4	8.170869217067311E-5
12	8.170869217067311E-5	0.0	0.0	8.170869217067312E-4	8.170869217067311E-5
13	8.170869217067311E-5	0.0	0.0	0.0012256303825600968	8.170869217067311E-5
14	8.170869217067311E-5	0.0	0.0	0.0017158825355841354	8.170869217067311E-5
15	8.170869217067311E-5	0.0	0.0	0.0024512607651201936	8.170869217067311E-5
16	8.170869217067311E-5	0.0	0.0	0.004330560685045675	8.170869217067311E-5
17	8.170869217067311E-5	0.0	0.0	0.0064549866814831765	8.170869217067311E-5
18	8.170869217067311E-5	0.0	0.0	0.0075989083718726	8.170869217067311E-5
19	8.170869217067311E-5	0.0	0.0	0.008415995293579332	8.170869217067311E-5
20	8.170869217067311E-5	0.0	0.0	0.009886751752651447	8.170869217067311E-5
21	8.170869217067311E-5	0.0	0.0	0.012011177749088948	8.170869217067311E-5
22	8.170869217067311E-5	0.0	0.0	0.01495269066723318	8.170869217067311E-5
23	8.170869217067311E-5	0.0	0.0	0.021080842580033662	8.170869217067311E-5
24	8.170869217067311E-5	0.0	0.0	0.03170297256222117	8.170869217067311E-5
25	8.170869217067311E-5	0.0	0.0	0.03529815501773079	8.170869217067311E-5
26	8.170869217067311E-5	0.0	0.0	0.0477178762276731	8.170869217067311E-5
27	8.170869217067311E-5	0.0	0.0	0.10205415652117072	8.170869217067311E-5
28	1.6341738434134622E-4	0.0	0.0	0.3009331132645891	8.170869217067311E-5
29	1.6341738434134622E-4	0.0	0.0	0.5765365319562695	8.170869217067311E-5
30	1.6341738434134622E-4	0.0	0.0	0.9479842465641495	8.170869217067311E-5
31	1.6341738434134622E-4	0.0	0.0	1.6905528410112267	8.170869217067311E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	100	5.347829E-10	22.2	1
GCAGTCG	790	0.0	19.202532	9
CAGTCGG	795	0.0	19.08176	10
AGTCGGT	805	0.0	19.074535	11
TCCCGTA	50	0.007035135	18.5	2
GGACCGT	50	0.007035135	18.5	6
CGGTGAT	850	0.0	17.84706	14
GTGATTC	850	0.0	17.629412	16
GGCAGTC	865	0.0	17.53757	8
ATACACA	560	0.0	17.50893	37
CGGTCCA	350	0.0	17.442858	10
TTCTAGC	85	2.7233482E-5	17.411764	2
GGTATCA	2750	0.0	17.356363	1
CTCTATG	1365	0.0	17.212454	1
TGCATCA	745	0.0	17.134228	14
CAGGACA	880	0.0	17.028408	4
TCGGTGA	880	0.0	17.028408	13
CCGCTTA	295	0.0	16.932201	25
GTCGGTG	875	0.0	16.914286	12
GTACTAT	165	5.4569682E-11	16.81818	1
>>END_MODULE