FastQCFastQC Report
Fri 10 Feb 2017
ERR1631362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4999286
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT69790.13959993487069952No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA69640.1392998920245811No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT65630.1312787466050152No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC59690.11939704989872553No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC55180.11037576165876486No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC53850.10771538175651484No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC52630.10527503327475163No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG52180.10437490473639635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.023.7857131
CTTATAC57600.016.7013937
AAGACGG18050.016.6038785
GTATCAA45900.016.5250532
CGCGAAC2050.016.24390428
ATACACA15850.016.10725637
TCGTTTA16800.015.41666630
TATTAGA8800.014.9261362
GTTCAAA22350.014.6510081
TTAACGG5450.014.5963335
TGGATAC21650.014.44110924
TATACTG2700.014.3888895
ATACCGC20350.014.36363627
TATACAC20500.014.16829237
TCTAGCG19600.014.15816428
ATTTCGG7350.014.09523929
TCTTATA88350.014.029428537
GGATACC22050.014.01133825
CGTTTAT18500.013.931
CGAGCCG23650.013.76744315
Produced by FastQC (version 0.11.5)