##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631362.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4999286
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07278199326864	34.0	33.0	34.0	31.0	34.0
2	33.21306802611413	34.0	33.0	34.0	31.0	34.0
3	33.29849202466112	34.0	34.0	34.0	31.0	34.0
4	36.541553533844635	37.0	37.0	37.0	35.0	37.0
5	36.519093526555594	37.0	37.0	37.0	35.0	37.0
6	36.562738959123365	37.0	37.0	37.0	35.0	37.0
7	36.56064165962899	37.0	37.0	37.0	35.0	37.0
8	36.55273633074803	37.0	37.0	37.0	35.0	37.0
9	38.40339980549222	39.0	39.0	39.0	37.0	39.0
10	38.362219124891034	39.0	39.0	39.0	37.0	39.0
11	38.41841374948343	39.0	39.0	39.0	37.0	39.0
12	38.37236477368968	39.0	39.0	39.0	37.0	39.0
13	38.405117650800534	39.0	39.0	39.0	37.0	39.0
14	39.95381120423997	41.0	40.0	41.0	38.0	41.0
15	39.954290472679496	41.0	40.0	41.0	38.0	41.0
16	39.92632447913562	41.0	40.0	41.0	38.0	41.0
17	39.90820009097299	41.0	40.0	41.0	38.0	41.0
18	39.87848324740773	41.0	40.0	41.0	38.0	41.0
19	39.88840646444312	41.0	40.0	41.0	38.0	41.0
20	39.85882364001579	41.0	40.0	41.0	38.0	41.0
21	39.82455814690338	41.0	40.0	41.0	38.0	41.0
22	39.77618083862376	41.0	40.0	41.0	38.0	41.0
23	39.71066728328805	41.0	40.0	41.0	37.0	41.0
24	39.68482239263767	41.0	40.0	41.0	37.0	41.0
25	39.66222436563941	41.0	40.0	41.0	37.0	41.0
26	39.571492609144585	41.0	40.0	41.0	37.0	41.0
27	39.518336418440555	41.0	40.0	41.0	37.0	41.0
28	39.4401418522565	41.0	39.0	41.0	36.0	41.0
29	39.38382621038284	41.0	39.0	41.0	36.0	41.0
30	39.31871971317504	41.0	39.0	41.0	36.0	41.0
31	39.27200964297702	41.0	39.0	41.0	35.0	41.0
32	39.20720178841538	41.0	39.0	41.0	35.0	41.0
33	39.12483922704162	41.0	39.0	41.0	35.0	41.0
34	39.07319505225346	41.0	39.0	41.0	35.0	41.0
35	38.96874053614856	41.0	39.0	41.0	35.0	41.0
36	38.907319965291045	40.0	39.0	41.0	35.0	41.0
37	38.844148344383576	40.0	38.0	41.0	35.0	41.0
38	38.76509205514547	40.0	38.0	41.0	35.0	41.0
39	38.68392726481341	40.0	38.0	41.0	35.0	41.0
40	38.57980819661048	40.0	38.0	41.0	35.0	41.0
41	38.49828215469169	40.0	38.0	41.0	35.0	41.0
42	38.367158830280964	40.0	37.0	41.0	35.0	41.0
43	37.5540245147007	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	4.0
13	3.0
14	8.0
15	6.0
16	23.0
17	31.0
18	88.0
19	157.0
20	305.0
21	620.0
22	1100.0
23	1818.0
24	2945.0
25	4433.0
26	6272.0
27	9048.0
28	12602.0
29	17181.0
30	23241.0
31	30549.0
32	40504.0
33	55132.0
34	86013.0
35	130189.0
36	217574.0
37	418639.0
38	1006443.0
39	2934351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2274328774149	17.83402669901262	11.801345232099143	27.13719519147334
2	20.185882544027287	19.170937609890693	32.70497026975452	27.9382095763275
3	20.297178437080817	20.80295066135444	28.336126398849753	30.56374450271499
4	15.075652803220299	15.04010772738347	32.97952947680929	36.904709992586945
5	17.15248937548282	33.45427727079427	32.14741065024086	17.24582270348206
6	36.02030369936827	33.27591180020507	15.088114582762419	15.615669917664244
7	31.37570045002426	28.40197580214455	20.170340324598353	20.051983423232837
8	28.524473294786496	30.799438159769217	19.22180487373597	21.45428367170832
9	27.283316057533014	13.90774602613253	19.091386249956493	39.71755166637796
10	19.44159625994592	23.717606874261644	30.03766937918735	26.80312748660509
11	39.04281531402684	19.940287473051153	19.898041440317677	21.118855772604327
12	23.984384970173743	23.766093798194383	26.225284970693814	26.02423626093806
13	31.42028681695746	17.320733400729623	23.248359865788835	28.01061991652408
14	24.253563408854784	19.362284934288617	22.42572239315774	33.95842926369886
15	27.517569508925877	25.481598772304686	20.109591649687573	26.891240069081867
16	27.784307599125157	23.747931204576016	21.88542523872409	26.58233595757474
17	26.40847112967732	24.086799594982164	22.89378923310249	26.610940042238028
18	26.76518206799931	22.29774411786003	24.234740720974955	26.702333093165702
19	26.882018752277826	23.255980954080243	23.419824350917313	26.44217594272462
20	26.887259500656697	22.44146464115076	23.304547889438613	27.36672796875394
21	28.381092820054704	23.030328730942777	22.892969115989764	25.695609333012754
22	28.12737658937696	22.475289471336506	22.79065450546338	26.60667943382315
23	26.487322389637242	22.294843703680886	23.704164954755537	27.513668951926334
24	26.490262809529202	23.55896422009063	22.916392460843408	27.03438050953676
25	26.900281360178234	22.333429213691716	23.26826270791469	27.498026718215364
26	26.685570699495887	23.77057443802975	23.37011725274369	26.17373760973067
27	27.397072301924712	22.49769267051335	22.931074557446802	27.174160470115133
28	26.170417135566957	22.91647247226904	24.20891703335236	26.70419335881164
29	25.827988236720206	23.287825501481613	24.199575699409877	26.68461056238831
30	25.53674664742125	23.66435927050383	24.30995146106864	26.488942621006277
31	26.95048852976205	23.16124742613245	22.89210899316422	26.996155050941272
32	25.863433298274995	22.697941266012787	23.30772834360747	28.13089709210475
33	25.349519911443352	22.24411645983046	24.4198071484608	27.986556480265385
34	26.93126578475406	22.00052167449512	24.23288045532902	26.835332085421797
35	26.87091716697144	22.25087742529633	24.769457078470804	26.108748329261417
36	25.30291325601296	23.008205571755646	24.484936448924906	27.20394472330649
37	25.91070004796685	21.810774578609827	25.122367474075297	27.156157899348027
38	25.125427911105707	21.42719980413203	26.270171380473133	27.177200904289133
39	25.242184583958586	21.21508951478271	26.177378129596907	27.365347771661792
40	24.869851414782033	21.935852439728393	26.882778860821325	26.311517284668252
41	23.904133510265265	21.979178626707892	27.613083148273574	26.503604714753266
42	24.691645967044092	22.531877552114445	27.116212195101458	25.660264285740002
43	23.583067662062142	21.309042931330595	27.450099874262047	27.657789532345216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	257.5
2	272.0
3	686.5
4	1101.0
5	1101.0
6	1276.5
7	1452.0
8	1294.5
9	1137.0
10	1653.5
11	2170.0
12	2170.0
13	3938.0
14	5706.0
15	7921.5
16	10137.0
17	9793.0
18	9449.0
19	9449.0
20	11277.0
21	13105.0
22	12563.5
23	12022.0
24	15083.0
25	18144.0
26	18144.0
27	23095.0
28	28046.0
29	37193.5
30	46341.0
31	58958.0
32	71575.0
33	71575.0
34	93955.5
35	116336.0
36	144531.5
37	172727.0
38	198853.5
39	224980.0
40	224980.0
41	245308.0
42	265636.0
43	279653.0
44	293670.0
45	305480.5
46	317291.0
47	317291.0
48	332060.5
49	346830.0
50	356427.0
51	366024.0
52	374051.0
53	382078.0
54	382078.0
55	379541.5
56	377005.0
57	364046.0
58	351087.0
59	347804.5
60	344522.0
61	344522.0
62	320971.5
63	297421.0
64	273387.0
65	249353.0
66	223655.0
67	197957.0
68	197957.0
69	174230.0
70	150503.0
71	133659.5
72	116816.0
73	92077.5
74	67339.0
75	67339.0
76	55149.0
77	42959.0
78	36338.0
79	29717.0
80	26844.5
81	23972.0
82	23972.0
83	19690.0
84	15408.0
85	14347.5
86	13287.0
87	11751.0
88	10215.0
89	10215.0
90	7336.5
91	4458.0
92	2502.5
93	547.0
94	359.5
95	172.0
96	172.0
97	114.0
98	56.0
99	38.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4999286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.469085260706976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.64061420001445	36.357809899183266
2	9.078371971059129	7.892570504767635
3	2.6247860790477304	3.4229114958372766
4	1.2754090751959248	2.217634633278844
5	0.7151451827499773	1.5543353461371316
6	0.464729742057096	1.212082606839976
7	0.3394463515134167	1.0328795676760834
8	0.24508933973857355	0.8523047524469144
9	0.19454230975853598	0.76109186247378
>10	1.1304103463745951	9.440223866069628
>50	0.12855659932208607	3.919065235256732
>100	0.12685731104538514	11.526773019513351
>500	0.01945966513202576	5.905331826769488
>1k	0.016216387277665608	12.954665369868264
>5k	3.6543971329950617E-4	0.9503200138816675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6979	0.13959993487069952	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	6964	0.1392998920245811	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	6563	0.1312787466050152	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	5969	0.11939704989872553	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	5518	0.11037576165876486	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	5385	0.10771538175651484	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	5263	0.10527503327475163	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	5218	0.10437490473639635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.00057128157901E-5
2	0.0	0.0	0.0	0.0	4.00057128157901E-5
3	0.0	0.0	0.0	0.0	4.00057128157901E-5
4	0.0	2.000285640789505E-5	0.0	0.0	4.00057128157901E-5
5	0.0	6.000856922368514E-5	0.0	0.0	4.00057128157901E-5
6	0.0	6.000856922368514E-5	0.0	0.0	6.000856922368514E-5
7	0.0	6.000856922368514E-5	0.0	0.0	6.000856922368514E-5
8	0.0	6.000856922368514E-5	0.0	0.0	8.00114256315802E-5
9	0.0	6.000856922368514E-5	0.0	2.000285640789505E-5	8.00114256315802E-5
10	0.0	8.00114256315802E-5	0.0	2.000285640789505E-5	8.00114256315802E-5
11	0.0	8.00114256315802E-5	0.0	2.000285640789505E-5	8.00114256315802E-5
12	0.0	8.00114256315802E-5	0.0	8.00114256315802E-5	8.00114256315802E-5
13	0.0	8.00114256315802E-5	0.0	8.00114256315802E-5	8.00114256315802E-5
14	0.0	8.00114256315802E-5	0.0	1.600228512631604E-4	8.00114256315802E-5
15	0.0	8.00114256315802E-5	0.0	3.400485589342158E-4	1.0001428203947524E-4
16	0.0	8.00114256315802E-5	0.0	5.400771230131663E-4	1.0001428203947524E-4
17	0.0	8.00114256315802E-5	0.0	8.601228255394871E-4	1.0001428203947524E-4
18	0.0	8.00114256315802E-5	0.0	0.0012001713844737028	1.2001713844737028E-4
19	0.0	8.00114256315802E-5	0.0	0.0015402199434079186	1.2001713844737028E-4
20	0.0	8.00114256315802E-5	0.0	0.002040291353605295	1.2001713844737028E-4
21	0.0	8.00114256315802E-5	0.0	0.0025603656202105663	1.4001999485526532E-4
22	0.0	8.00114256315802E-5	0.0	0.0036005141534211087	1.4001999485526532E-4
23	0.0	1.0001428203947524E-4	0.0	0.005960851209552724	1.600228512631604E-4
24	0.0	1.4001999485526532E-4	0.0	0.008821259675881717	1.600228512631604E-4
25	0.0	1.4001999485526532E-4	0.0	0.011321616726868596	1.600228512631604E-4
26	0.0	1.4001999485526532E-4	0.0	0.018442633608079233	2.0002856407895048E-4
27	0.0	1.4001999485526532E-4	0.0	0.04894698963011918	2.2003142048684552E-4
28	0.0	1.4001999485526532E-4	0.0	0.16758393098534471	2.2003142048684552E-4
29	0.0	2.0002856407895048E-4	0.0	0.31124444570684695	2.4003427689474056E-4
30	0.0	2.0002856407895048E-4	0.0	0.5030318329457446	2.4003427689474056E-4
31	0.0	2.0002856407895048E-4	0.0	1.0189655082745817	2.4003427689474056E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3150	0.0	23.785713	1
CTTATAC	5760	0.0	16.70139	37
AAGACGG	1805	0.0	16.603878	5
GTATCAA	4590	0.0	16.525053	2
CGCGAAC	205	0.0	16.243904	28
ATACACA	1585	0.0	16.107256	37
TCGTTTA	1680	0.0	15.416666	30
TATTAGA	880	0.0	14.926136	2
GTTCAAA	2235	0.0	14.651008	1
TTAACGG	545	0.0	14.59633	35
TGGATAC	2165	0.0	14.441109	24
TATACTG	270	0.0	14.388889	5
ATACCGC	2035	0.0	14.363636	27
TATACAC	2050	0.0	14.168292	37
TCTAGCG	1960	0.0	14.158164	28
ATTTCGG	735	0.0	14.095239	29
TCTTATA	8835	0.0	14.0294285	37
GGATACC	2205	0.0	14.011338	25
CGTTTAT	1850	0.0	13.9	31
CGAGCCG	2365	0.0	13.767443	15
>>END_MODULE