FastQCFastQC Report
Fri 10 Feb 2017
ERR1631361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302513
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA37290.28629272798045013No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC33150.25450801642670745No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC31000.23800146332512612No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC26120.20053542651781595No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC24370.18709986003978463No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC23510.1804972387991521No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT19130.14686993527127945No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG18640.14310797665743066No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17770.1364285807512094No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT17360.13328081946207063No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16120.12376076092906559No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC15990.12276269027641183No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG15140.11623684370136804No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15120.11608329437019056No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG15080.11577619570783554No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG15040.11546909704548054No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC14930.1146245757240043No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14920.11454780105841554No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC14830.11385682906811678No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT14630.11232133575634178No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC14410.11063229311338928No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG14320.10994132112309052No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC14260.10948067312955802No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG14220.10917357446720301No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC14130.10848260247690426No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG13900.106716785168363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA200.001842026137.01
GGTATAT502.7023698E-422.21
GGTATCA10600.021.2924541
GTAAGCT450.003826078520.55555528
TATTAGA1001.2902092E-820.352
TAGAGCA1052.2600943E-819.380954
TGCATCA3650.018.75342614
TCGTTTA3950.018.73417730
TAAGTAA701.2193555E-418.54
GCATCAG3800.018.515
GTTCAAA6850.018.3649641
AGTCGGT5150.018.32038711
GCAGTCG5450.017.9908269
TTAGAGT3200.017.9218754
CGGTGAT5100.017.7745114
TAGGGGT1156.4068445E-817.6956524
TATACAC3050.017.59016237
CTAGACA852.7235674E-517.4117644
CTATCCG752.0675329E-417.2666669
ACTCTAA3350.017.11940410
Produced by FastQC (version 0.11.5)