##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302513
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07435319263608	34.0	33.0	34.0	31.0	34.0
2	33.21780742303532	34.0	33.0	34.0	31.0	34.0
3	33.299071871067696	34.0	34.0	34.0	31.0	34.0
4	36.52619743526552	37.0	37.0	37.0	35.0	37.0
5	36.52197098992486	37.0	37.0	37.0	35.0	37.0
6	36.568872633133026	37.0	37.0	37.0	35.0	37.0
7	36.569281842100615	37.0	37.0	37.0	35.0	37.0
8	36.55854413737137	37.0	37.0	37.0	35.0	37.0
9	38.41943458529781	39.0	39.0	39.0	37.0	39.0
10	38.37250453546337	39.0	39.0	39.0	37.0	39.0
11	38.43596724178569	39.0	39.0	39.0	37.0	39.0
12	38.39297803553592	39.0	39.0	39.0	37.0	39.0
13	38.415973583373066	39.0	39.0	39.0	37.0	39.0
14	39.971164971098176	41.0	40.0	41.0	38.0	41.0
15	39.97768697893994	41.0	40.0	41.0	38.0	41.0
16	39.95429757706833	41.0	40.0	41.0	38.0	41.0
17	39.932419100615505	41.0	40.0	41.0	38.0	41.0
18	39.88631898491608	41.0	40.0	41.0	38.0	41.0
19	39.90743585668627	41.0	40.0	41.0	38.0	41.0
20	39.88952432720441	41.0	40.0	41.0	38.0	41.0
21	39.857734241424076	41.0	40.0	41.0	38.0	41.0
22	39.80578927043339	41.0	40.0	41.0	38.0	41.0
23	39.747181026216246	41.0	40.0	41.0	38.0	41.0
24	39.713151423440685	41.0	40.0	41.0	37.0	41.0
25	39.6941335710277	41.0	40.0	41.0	37.0	41.0
26	39.62007442536082	41.0	40.0	41.0	37.0	41.0
27	39.56718205499676	41.0	40.0	41.0	37.0	41.0
28	39.46889666360336	41.0	40.0	41.0	37.0	41.0
29	39.41411717195913	41.0	39.0	41.0	36.0	41.0
30	39.356080899000624	41.0	39.0	41.0	36.0	41.0
31	39.32456182778982	41.0	39.0	41.0	36.0	41.0
32	39.261164379933255	41.0	39.0	41.0	35.0	41.0
33	39.16661484376739	41.0	39.0	41.0	35.0	41.0
34	39.114577743177996	41.0	39.0	41.0	35.0	41.0
35	39.00103415474548	41.0	39.0	41.0	35.0	41.0
36	38.94357676276552	40.0	39.0	41.0	35.0	41.0
37	38.87631217500324	40.0	38.0	41.0	35.0	41.0
38	38.7922554323834	40.0	38.0	41.0	35.0	41.0
39	38.69937728068741	40.0	38.0	41.0	35.0	41.0
40	38.59619903985603	40.0	38.0	41.0	35.0	41.0
41	38.50756499167379	40.0	37.0	41.0	35.0	41.0
42	38.37401162214888	40.0	37.0	41.0	35.0	41.0
43	37.53058203641729	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	1.0
16	3.0
17	13.0
18	27.0
19	44.0
20	91.0
21	140.0
22	260.0
23	449.0
24	667.0
25	1064.0
26	1563.0
27	2172.0
28	2944.0
29	4097.0
30	5591.0
31	7476.0
32	9819.0
33	13712.0
34	22345.0
35	34742.0
36	56906.0
37	107899.0
38	263157.0
39	767328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23871393222179	16.619027986668847	11.253323383336673	27.888934697772687
2	20.7386797674956	18.810176942571783	31.403525339094507	29.047617950838113
3	20.80332403592133	20.235728933223697	28.184593934954965	30.7763530959
4	14.539048746538421	14.37828259679558	32.85909622399162	38.22357243267437
5	16.795532942857385	34.55205437488916	31.922214979812104	16.73019770244136
6	37.145579353142736	32.85011358811774	15.028410465001116	14.97589659373841
7	32.28159718943304	28.150352434102384	19.37017135337613	20.197879023088444
8	30.854740029466114	29.24155075611529	18.51367318406803	21.39003603035056
9	28.391578433382236	12.895687029611222	17.23568210067769	41.47705243632885
10	19.24533574712882	23.700953464571946	29.694214184426563	27.359496603872667
11	40.141173255084595	19.826903838963602	18.74392040616869	21.28800249978311
12	25.01310927414928	22.975509649423845	25.545848678669618	26.465532397757258
13	32.26969711626679	16.22386878288355	23.370361754546785	28.136072346302875
14	24.55860325386388	18.558739912768623	21.43556340704469	35.44709342632281
15	28.570386629538437	24.675531069555543	19.237811829901123	27.516270471004894
16	28.7262392006836	23.089443253157548	20.67549421771606	27.508823328442787
17	27.53676930671709	23.33504540837596	21.58051397567625	27.547671309230694
18	27.642488021232804	20.795493020031277	23.601453497968926	27.960565460766993
19	27.87603655395378	22.594630533438053	21.86074150507519	27.66859140753298
20	27.807553552248613	21.156871370957525	22.19125644043476	28.8443186363591
21	30.25313374991267	22.04254391318935	21.433951139067325	26.270371197830656
22	29.84246606367844	21.228962781945363	21.28439409050044	27.644177063875752
23	27.689320567241936	21.213991722155555	22.04323488517965	29.053452825422855
24	26.9989627742679	22.52791334904143	22.279854404524176	28.1932694721665
25	27.712352966918562	21.302820010241742	22.004617228388508	28.980209794451188
26	28.224516761061118	22.71355449043503	22.152101360984496	26.909827387519357
27	29.29022589409856	21.027966707434015	21.780435204869356	27.901372193598068
28	27.06383736669039	21.71218252716096	22.877007753473478	28.346972352675177
29	26.230985794383628	21.935903902686576	23.74371695330488	28.08939334962492
30	25.519591743038266	23.43162793768661	23.793850809934337	27.25492950934079
31	27.80609483360243	22.90449308375425	21.50066832346395	27.78874375917937
32	26.77631624406052	22.045538125147313	22.492673777536194	28.685471853255972
33	26.314439855878597	21.675714561006302	23.00944405161407	29.00040153150103
34	27.898224432308925	21.975980278123902	22.81113509039833	27.314660199168838
35	27.802486424319756	21.725925192301343	23.684446911470367	26.787141471908534
36	26.05394341553597	23.635541449490333	22.787411718731406	27.523103416242297
37	27.606634252402856	21.55295187072989	23.563987461161616	27.27642641570564
38	26.08833846571973	21.173070825396753	24.74762248054338	27.99096822834014
39	26.74069279922734	21.01606663426776	24.29234871360209	27.95089185290281
40	25.95298473028676	21.00370591310797	25.269997305209234	27.773312051396033
41	24.469698191112105	21.39134119966557	26.661077471011808	27.47788313821052
42	26.07843453385878	20.987506458668744	26.548372261927522	26.385686745544955
43	24.004827590972223	19.709822473940758	27.154738570747472	29.130611364339547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	10.0
2	13.0
3	44.0
4	75.0
5	75.0
6	110.0
7	145.0
8	127.0
9	109.0
10	172.5
11	236.0
12	236.0
13	441.0
14	646.0
15	1160.0
16	1674.0
17	1595.0
18	1516.0
19	1516.0
20	1821.5
21	2127.0
22	2083.5
23	2040.0
24	2635.0
25	3230.0
26	3230.0
27	4199.0
28	5168.0
29	6996.0
30	8824.0
31	11164.0
32	13504.0
33	13504.0
34	17071.5
35	20639.0
36	26352.0
37	32065.0
38	38699.0
39	45333.0
40	45333.0
41	49945.0
42	54557.0
43	57806.0
44	61055.0
45	67514.5
46	73974.0
47	73974.0
48	82550.0
49	91126.0
50	94690.0
51	98254.0
52	105160.0
53	112066.0
54	112066.0
55	108398.0
56	104730.0
57	102282.5
58	99835.0
59	97727.5
60	95620.0
61	95620.0
62	93230.0
63	90840.0
64	85738.5
65	80637.0
66	72764.0
67	64891.0
68	64891.0
69	54853.0
70	44815.0
71	39628.5
72	34442.0
73	27834.0
74	21226.0
75	21226.0
76	18420.0
77	15614.0
78	12612.0
79	9610.0
80	7420.0
81	5230.0
82	5230.0
83	4019.5
84	2809.0
85	2370.0
86	1931.0
87	1538.0
88	1145.0
89	1145.0
90	868.0
91	591.0
92	349.0
93	107.0
94	72.0
95	37.0
96	37.0
97	24.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1302513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.79778621396819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43818881384236	40.91913667046779
2	6.79090917356938	6.355990314063504
3	1.8598866918554582	2.611157393629688
4	0.9309492159425463	1.7426544953496235
5	0.5179574527173357	1.2119631070098706
6	0.36008162336632654	1.0110613697925577
7	0.2513236922409541	0.8232974693998113
8	0.19124531798383226	0.7159886004343798
9	0.14855203140391043	0.6256715586561801
>10	1.1408880271697004	10.933346316494958
>50	0.17811853207174067	5.867233216561387
>100	0.16706925462000224	16.024479687081726
>500	0.016608261555443805	5.34711067040389
>1k	0.008221911661110796	5.810909130654653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3729	0.28629272798045013	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3315	0.25450801642670745	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	3100	0.23800146332512612	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2612	0.20053542651781595	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2437	0.18709986003978463	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	2351	0.1804972387991521	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1913	0.14686993527127945	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1864	0.14310797665743066	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1777	0.1364285807512094	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	1736	0.13328081946207063	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1612	0.12376076092906559	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1599	0.12276269027641183	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1514	0.11623684370136804	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1512	0.11608329437019056	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	1508	0.11577619570783554	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	1504	0.11546909704548054	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1493	0.1146245757240043	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1492	0.11454780105841554	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1483	0.11385682906811678	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1463	0.11232133575634178	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	1441	0.11063229311338928	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1432	0.10994132112309052	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1426	0.10948067312955802	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1422	0.10917357446720301	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	1413	0.10848260247690426	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	1390	0.106716785168363	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.677466558875037E-5	0.0	0.0	0.0
3	0.0	1.5354933117750074E-4	0.0	0.0	0.0
4	0.0	1.5354933117750074E-4	0.0	0.0	0.0
5	0.0	2.3032399676625108E-4	0.0	0.0	0.0
6	0.0	2.3032399676625108E-4	0.0	0.0	0.0
7	0.0	2.3032399676625108E-4	0.0	0.0	0.0
8	0.0	2.3032399676625108E-4	0.0	0.0	0.0
9	0.0	2.3032399676625108E-4	0.0	7.677466558875037E-5	0.0
10	7.677466558875037E-5	2.3032399676625108E-4	0.0	1.5354933117750074E-4	0.0
11	7.677466558875037E-5	2.3032399676625108E-4	0.0	1.5354933117750074E-4	0.0
12	7.677466558875037E-5	2.3032399676625108E-4	0.0	1.5354933117750074E-4	0.0
13	7.677466558875037E-5	2.3032399676625108E-4	0.0	2.3032399676625108E-4	0.0
14	7.677466558875037E-5	2.3032399676625108E-4	0.0	2.3032399676625108E-4	0.0
15	7.677466558875037E-5	2.3032399676625108E-4	0.0	5.374226591212526E-4	0.0
16	7.677466558875037E-5	2.3032399676625108E-4	0.0	0.001228394649420006	0.0
17	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.0018425919741300086	0.0
18	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.00207291597089626	0.0
19	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.002380014633251261	0.0
20	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.002917437292372514	0.0
21	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.003992282610615018	7.677466558875037E-5
22	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.005297451925623775	7.677466558875037E-5
23	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.007831015890052537	7.677466558875037E-5
24	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.010211030523303797	7.677466558875037E-5
25	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.012053622497433808	7.677466558875037E-5
26	1.5354933117750074E-4	2.3032399676625108E-4	0.0	0.016276229104815076	7.677466558875037E-5
27	2.3032399676625108E-4	2.3032399676625108E-4	0.0	0.03024921824196764	7.677466558875037E-5
28	2.3032399676625108E-4	3.070986623550015E-4	0.0	0.0858340761282229	1.5354933117750074E-4
29	2.3032399676625108E-4	4.6064799353250216E-4	0.0	0.17949916814649836	1.5354933117750074E-4
30	2.3032399676625108E-4	4.6064799353250216E-4	0.0	0.29412374387050266	1.5354933117750074E-4
31	2.3032399676625108E-4	4.6064799353250216E-4	0.0	0.5722015826329564	1.5354933117750074E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	20	0.0018420261	37.0	1
GGTATAT	50	2.7023698E-4	22.2	1
GGTATCA	1060	0.0	21.292454	1
GTAAGCT	45	0.0038260785	20.555555	28
TATTAGA	100	1.2902092E-8	20.35	2
TAGAGCA	105	2.2600943E-8	19.38095	4
TGCATCA	365	0.0	18.753426	14
TCGTTTA	395	0.0	18.734177	30
TAAGTAA	70	1.2193555E-4	18.5	4
GCATCAG	380	0.0	18.5	15
GTTCAAA	685	0.0	18.364964	1
AGTCGGT	515	0.0	18.320387	11
GCAGTCG	545	0.0	17.990826	9
TTAGAGT	320	0.0	17.921875	4
CGGTGAT	510	0.0	17.77451	14
TAGGGGT	115	6.4068445E-8	17.695652	4
TATACAC	305	0.0	17.590162	37
CTAGACA	85	2.7235674E-5	17.411764	4
CTATCCG	75	2.0675329E-4	17.266666	9
ACTCTAA	335	0.0	17.119404	10
>>END_MODULE