FastQCFastQC Report
Fri 10 Feb 2017
ERR1631360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110683
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1730.15630223250182956No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1370.12377691244364536No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1360.12287343133091802No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1310.11835602576728134No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1300.117452544654554No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1240.11203165797818997No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1220.11022469575273529No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC1130.10209336573818925No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1120.10118988462546191No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1120.10118988462546191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATG358.818626E-426.428571
TCTATGG358.818626E-426.428572
TATACAC809.549694E-1025.437537
ACGGCTA400.00192023823.12524
ACAGAGG400.00192023823.1258
CGGCTAC400.00192023823.12525
TACCGGC400.00192023823.1252
GGTATCA652.6466496E-622.7692281
TACCACA502.6807789E-422.19999929
TAAGGGC603.687264E-521.58333429
TCCATTG705.0335184E-621.1428572
CATTGGA806.843911E-720.81254
CGTGCGT450.00380432320.5555579
TTAGACA450.00380432320.55555718
AAACGGC450.00380432320.55555722
ACCACAT450.00380432320.55555730
TGAGAAA450.00380432320.55555718
CTCAGGA450.00380432320.5555572
ATCTAAG656.830365E-519.92307526
CATCTAA656.830365E-519.92307525