##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631360.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110683
Sequences flagged as poor quality	0
Sequence length	43
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05082081259091	34.0	33.0	34.0	31.0	34.0
2	33.193823803113396	34.0	33.0	34.0	31.0	34.0
3	33.28656613933486	34.0	34.0	34.0	31.0	34.0
4	36.5384114995076	37.0	37.0	37.0	35.0	37.0
5	36.51791151305982	37.0	37.0	37.0	35.0	37.0
6	36.55777309975335	37.0	37.0	37.0	35.0	37.0
7	36.55079822556309	37.0	37.0	37.0	35.0	37.0
8	36.53914331920891	37.0	37.0	37.0	35.0	37.0
9	38.390945312288245	39.0	39.0	39.0	37.0	39.0
10	38.34566283891835	39.0	39.0	39.0	37.0	39.0
11	38.41080382714599	39.0	39.0	39.0	37.0	39.0
12	38.35720932753901	39.0	39.0	39.0	37.0	39.0
13	38.380808254203444	39.0	39.0	39.0	37.0	39.0
14	39.925273077166324	41.0	40.0	41.0	38.0	41.0
15	39.92789317239323	41.0	40.0	41.0	38.0	41.0
16	39.90854964176974	41.0	40.0	41.0	38.0	41.0
17	39.87707235980232	41.0	40.0	41.0	38.0	41.0
18	39.84367066306479	41.0	40.0	41.0	38.0	41.0
19	39.853419224271114	41.0	40.0	41.0	38.0	41.0
20	39.83369623158028	41.0	40.0	41.0	38.0	41.0
21	39.78824209679897	41.0	40.0	41.0	38.0	41.0
22	39.739327629355905	41.0	40.0	41.0	38.0	41.0
23	39.663841782387536	41.0	40.0	41.0	37.0	41.0
24	39.618396682417355	41.0	40.0	41.0	37.0	41.0
25	39.59360516068411	41.0	40.0	41.0	37.0	41.0
26	39.49700496011131	41.0	39.0	41.0	37.0	41.0
27	39.437881156094434	41.0	39.0	41.0	36.0	41.0
28	39.3642293757849	41.0	39.0	41.0	36.0	41.0
29	39.28778583883704	41.0	39.0	41.0	36.0	41.0
30	39.20234363000642	41.0	39.0	41.0	35.0	41.0
31	39.157169574370045	41.0	39.0	41.0	35.0	41.0
32	39.08379787320546	41.0	39.0	41.0	35.0	41.0
33	39.009197437727565	41.0	39.0	41.0	35.0	41.0
34	38.952395580170396	40.0	39.0	41.0	35.0	41.0
35	38.839415267023846	40.0	38.0	41.0	35.0	41.0
36	38.76903408834238	40.0	38.0	41.0	35.0	41.0
37	38.693873494574596	40.0	38.0	41.0	35.0	41.0
38	38.62456745841728	40.0	38.0	41.0	35.0	41.0
39	38.54396790835088	40.0	38.0	41.0	35.0	41.0
40	38.44278705853654	40.0	37.0	41.0	35.0	41.0
41	38.35700152688308	40.0	37.0	41.0	35.0	41.0
42	38.230731006568305	40.0	37.0	41.0	34.0	41.0
43	37.37258657607763	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	2.0
20	8.0
21	17.0
22	22.0
23	39.0
24	66.0
25	94.0
26	136.0
27	207.0
28	292.0
29	401.0
30	483.0
31	705.0
32	913.0
33	1303.0
34	2059.0
35	3162.0
36	5374.0
37	9939.0
38	23418.0
39	62039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37266788937777	16.031368864233894	11.264602513484457	28.331360732903875
2	21.914837870314322	17.860014636394027	30.161813467289466	30.063334026002188
3	21.513692256263383	18.573764715448622	27.20110586088198	32.71143716740602
4	16.16508406891754	14.140382895295575	30.498811922336767	39.19572111345012
5	18.970392923935925	31.90553201485323	30.951455959813163	18.172619101397686
6	39.59686672750106	30.768952775042237	13.975949332779198	15.658231164677503
7	34.01244996973338	26.376227604961915	18.131058970212226	21.480263455092473
8	30.84574866962406	29.18243994109303	17.69558107387765	22.276230315405257
9	28.51115347433662	12.99747928769549	16.75776767886667	41.733599559101215
10	20.21810034061238	22.64575409051074	28.55813449219844	28.578011076678443
11	41.96398724284669	18.131058970212226	17.974756737710397	21.930197049230685
12	26.26238898475827	21.665477083201576	24.53131917277269	27.540814759267455
13	34.595195287442515	15.232691560582925	21.139651075594266	29.032462076380295
14	25.586585112438225	17.494104785739452	20.087095579266915	36.832214522555404
15	29.66760929862761	23.33420669840897	18.122024159084955	28.87615984387846
16	30.478031856744035	21.299567232547005	19.66065249405961	28.56174841664935
17	28.849055410496643	21.679029299892484	20.510828221136038	28.961087068474832
18	29.142686772133032	19.685949965215976	21.841655900183408	29.329707362467587
19	29.38030230478032	21.04568903987062	20.429514920990577	29.144493734358484
20	29.52576276392942	20.10606868263419	20.387954789805118	29.98021376363127
21	31.448370571813193	20.787293441630602	20.022044939150547	27.742291047405654
22	30.983981279871344	20.46294372216149	19.45104487590687	29.102030122060295
23	29.11919626320212	19.811533839885076	20.945402636357887	30.12386726055492
24	29.02071682191484	20.81801179946333	20.57316841791422	29.58810296070761
25	29.14901113992212	19.80069206653235	20.416866185412395	30.63343060813314
26	29.334224768031227	21.417019777201556	20.648157350270594	28.600598104496626
27	29.60346213962397	20.50269689112149	20.379823459790572	29.51401750946396
28	29.1417832910203	20.42138359097603	21.213736526837906	29.22309659116576
29	27.951898665558396	21.04388207764517	21.883216031368864	29.12100322542757
30	27.35469765004563	21.726913798867034	22.100051498423426	28.81833705266391
31	29.214061780038485	21.225481781303362	20.301220602983292	29.259235835674858
32	27.74500149074384	20.797231733870603	21.151396330059722	30.306370445325843
33	27.08726724067833	20.378919978677846	22.05758788612524	30.476224894518577
34	28.360272128511156	19.97687088351418	22.483127490219818	29.17972949775485
35	28.212101226023872	19.92988986565236	23.577243117732625	28.28076579059115
36	26.698770362205575	20.648157350270594	23.379380754045336	29.27369153347849
37	27.146896994118336	19.018277422910472	24.497890371601784	29.336935211369408
38	25.776316146110968	18.567440347659534	26.013028197645525	29.643215308583976
39	25.691388921514598	17.877180777535845	27.17942231417652	29.252007986773037
40	25.185439498387286	18.458119133019522	27.571533117100188	28.784908251493
41	23.945863411725377	18.532204584263166	29.44715990712214	28.074772096889316
42	23.875391884932647	18.777047965812272	29.38030230478032	27.967257844474762
43	21.89134736138341	17.850076344154026	30.22686410740583	30.03171218705673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	3.0
7	5.0
8	4.5
9	4.0
10	6.5
11	9.0
12	9.0
13	11.0
14	13.0
15	17.5
16	22.0
17	29.0
18	36.0
19	36.0
20	36.0
21	36.0
22	41.0
23	46.0
24	73.5
25	101.0
26	101.0
27	125.0
28	149.0
29	217.5
30	286.0
31	401.0
32	516.0
33	516.0
34	733.0
35	950.0
36	1304.5
37	1659.0
38	2108.5
39	2558.0
40	2558.0
41	2983.5
42	3409.0
43	3897.5
44	4386.0
45	4918.0
46	5450.0
47	5450.0
48	6116.0
49	6782.0
50	7417.0
51	8052.0
52	8724.5
53	9397.0
54	9397.0
55	9442.5
56	9488.0
57	9745.0
58	10002.0
59	10117.0
60	10232.0
61	10232.0
62	9725.5
63	9219.0
64	8641.5
65	8064.0
66	7237.0
67	6410.0
68	6410.0
69	5486.5
70	4563.0
71	3994.0
72	3425.0
73	2646.0
74	1867.0
75	1867.0
76	1532.5
77	1198.0
78	1028.5
79	859.0
80	744.0
81	629.0
82	629.0
83	516.5
84	404.0
85	306.5
86	209.0
87	181.5
88	154.0
89	154.0
90	112.5
91	71.0
92	44.0
93	17.0
94	10.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	110683.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.83412990251439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98660298951809	66.99764191429578
2	4.100973764187806	5.973817117353162
3	1.1796812007690876	2.5776316146110965
4	0.6041059356199219	1.7599812075928551
5	0.38330335545494015	1.3958783191637378
6	0.28158531290702726	1.230541275534635
7	0.16994355889102525	0.8664383871055176
8	0.15753891955591393	0.9179368105309758
9	0.12652732121813556	0.8293956614836968
>10	0.9303479501333499	12.886351110830027
>50	0.06326366060906778	3.115202876683863
>100	0.01612603113564473	1.4491837048146508
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	173	0.15630223250182956	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	137	0.12377691244364536	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	136	0.12287343133091802	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	131	0.11835602576728134	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	130	0.117452544654554	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	124	0.11203165797818997	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	122	0.11022469575273529	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	113	0.10209336573818925	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	112	0.10118988462546191	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	112	0.10118988462546191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	9.034811127273385E-4	0.0
22	0.0	0.0	0.0	9.034811127273385E-4	0.0
23	0.0	0.0	0.0	9.034811127273385E-4	0.0
24	0.0	0.0	0.0	0.001806962225454677	0.0
25	0.0	0.0	0.0	0.0027104433381820153	0.0
26	0.0	0.0	0.0	0.008131330014546046	0.0
27	0.0	0.0	0.0	0.05059494231273095	0.0
28	0.0	0.0	0.0	0.22406331595637993	0.0
29	0.0	0.0	0.0	0.5023354986764001	0.0
30	0.0	0.0	0.0	0.822167812581878	0.0
31	0.0	0.0	0.0	1.6642122096437575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTATG	35	8.818626E-4	26.42857	1
TCTATGG	35	8.818626E-4	26.42857	2
TATACAC	80	9.549694E-10	25.4375	37
ACGGCTA	40	0.001920238	23.125	24
ACAGAGG	40	0.001920238	23.125	8
CGGCTAC	40	0.001920238	23.125	25
TACCGGC	40	0.001920238	23.125	2
GGTATCA	65	2.6466496E-6	22.769228	1
TACCACA	50	2.6807789E-4	22.199999	29
TAAGGGC	60	3.687264E-5	21.583334	29
TCCATTG	70	5.0335184E-6	21.142857	2
CATTGGA	80	6.843911E-7	20.8125	4
CGTGCGT	45	0.003804323	20.555557	9
TTAGACA	45	0.003804323	20.555557	18
AAACGGC	45	0.003804323	20.555557	22
ACCACAT	45	0.003804323	20.555557	30
TGAGAAA	45	0.003804323	20.555557	18
CTCAGGA	45	0.003804323	20.555557	2
ATCTAAG	65	6.830365E-5	19.923075	26
CATCTAA	65	6.830365E-5	19.923075	25
>>END_MODULE