FastQCFastQC Report
Fri 10 Feb 2017
ERR1631359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376613
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10950.2907493899573302No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9890.2626037869112325No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8080.21454384208723543No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8000.21241964563092616No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC7500.19914341777899328No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7060.18746033726929234No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5650.15002137472684163No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5550.14736612915645503No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC5300.14072801523048858No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5080.13488647497563813No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC5060.13435542586156082No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT4920.1306380820630196No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT4800.12745178737855573No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT4770.12665521370743973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.12320339446593717No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG4350.1155031823118161No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG4340.11523765775477744No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC4210.11178583851327491No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG4090.108599543828811No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT4060.10780297015769502No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC3990.10594429825842443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3970.10541324914434712No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC3880.1030235281309992No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT3850.10222695445988322No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC3780.10036828256061261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACT451.3209059E-424.6666664
CTTAGGA701.915796E-723.7857132
ATACACA1250.023.6837
TATTGAC551.8982584E-523.54545430
TCAAAGC1200.023.1250023
GATACAC502.6974297E-422.23
GAATGTT1201.0913936E-1121.58333435
GTTCAAA1201.0913936E-1121.5833341
AATGTTT1201.0913936E-1121.58333436
AGTCGGT5250.021.49523711
TTGCGCG1550.021.4838718
TTTGCGC1550.021.4838717
ATTTGCG1550.021.4838716
GCAGTCG5100.021.401969
CAGTCGG5250.021.14285710
TGCGCGC1600.020.812519
GATTCCT5350.020.74766318
GCGAAAG1351.8189894E-1220.55555518
TCGGTGA5500.020.51818313
TTAGGAC1001.2829332E-820.353
Produced by FastQC (version 0.11.5)