##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376613
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.052369408384735	34.0	33.0	34.0	31.0	34.0
2	33.19348243422293	34.0	33.0	34.0	31.0	34.0
3	33.25784293160353	34.0	34.0	34.0	31.0	34.0
4	36.511477298977994	37.0	37.0	37.0	35.0	37.0
5	36.496291949560955	37.0	37.0	37.0	35.0	37.0
6	36.551866239349145	37.0	37.0	37.0	35.0	37.0
7	36.55474452554745	37.0	37.0	37.0	35.0	37.0
8	36.54385005297215	37.0	37.0	37.0	35.0	37.0
9	38.399611803097606	39.0	39.0	39.0	37.0	39.0
10	38.35828556104011	39.0	39.0	39.0	37.0	39.0
11	38.4199748813769	39.0	39.0	39.0	37.0	39.0
12	38.37913454925879	39.0	39.0	39.0	37.0	39.0
13	38.39759116121854	39.0	39.0	39.0	37.0	39.0
14	39.922161476104115	41.0	40.0	41.0	38.0	41.0
15	39.92919787686564	41.0	40.0	41.0	38.0	41.0
16	39.88754238435742	41.0	40.0	41.0	38.0	41.0
17	39.86487455292303	41.0	40.0	41.0	38.0	41.0
18	39.85631138595853	41.0	40.0	41.0	38.0	41.0
19	39.86986641459536	41.0	40.0	41.0	38.0	41.0
20	39.87122324508182	41.0	40.0	41.0	38.0	41.0
21	39.83522873612966	41.0	40.0	41.0	38.0	41.0
22	39.77906763707041	41.0	40.0	41.0	38.0	41.0
23	39.70338517257768	41.0	40.0	41.0	37.0	41.0
24	39.66531160634391	41.0	40.0	41.0	37.0	41.0
25	39.64804985489083	41.0	40.0	41.0	37.0	41.0
26	39.581533298107075	41.0	40.0	41.0	37.0	41.0
27	39.51625143051355	41.0	39.0	41.0	37.0	41.0
28	39.39081762976849	41.0	39.0	41.0	36.0	41.0
29	39.336942697145346	41.0	39.0	41.0	36.0	41.0
30	39.27090673980983	41.0	39.0	41.0	35.0	41.0
31	39.23608053890864	41.0	39.0	41.0	35.0	41.0
32	39.16330556831549	41.0	39.0	41.0	35.0	41.0
33	39.075305419621735	41.0	39.0	41.0	35.0	41.0
34	39.020357767788155	41.0	39.0	41.0	35.0	41.0
35	38.91139180007063	40.0	39.0	41.0	35.0	41.0
36	38.848021709287785	40.0	38.0	41.0	35.0	41.0
37	38.7869324744499	40.0	38.0	41.0	35.0	41.0
38	38.70063699341234	40.0	38.0	41.0	35.0	41.0
39	38.60310982361204	40.0	38.0	41.0	35.0	41.0
40	38.50729263195907	40.0	38.0	41.0	35.0	41.0
41	38.42234601567126	40.0	37.0	41.0	35.0	41.0
42	38.294830502399016	40.0	37.0	41.0	35.0	41.0
43	37.451973245745634	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	0.0
15	1.0
16	0.0
17	3.0
18	15.0
19	18.0
20	35.0
21	58.0
22	94.0
23	150.0
24	224.0
25	335.0
26	478.0
27	690.0
28	912.0
29	1281.0
30	1769.0
31	2310.0
32	2952.0
33	4278.0
34	6664.0
35	10129.0
36	17375.0
37	32834.0
38	77378.0
39	216627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45601452950376	16.221691763162717	12.501692719051121	27.8206009882824
2	21.61900943408751	18.7210744185676	31.255957707248555	28.403958440096332
3	20.784731275872048	19.914607302456368	28.43449376415578	30.866167657515803
4	14.918762761774024	15.374668426209398	32.12262986142274	37.58393895059385
5	17.254847814600133	34.30816249040792	31.527323804542064	16.90966589044988
6	36.55688996396832	32.01774766139246	15.642051655147327	15.783310719491892
7	32.03235151202959	28.21224970991442	19.02430346270575	20.731095315350238
8	30.53718273134491	29.083701306115294	18.48608518558839	21.893030776951406
9	28.190476696237248	13.430497619572346	17.4709848040296	40.9080408801608
10	20.241999081285034	23.1959597783401	28.346870660333025	28.215170480041845
11	39.67574141094439	20.334667151691523	17.995661328737988	21.9939301086261
12	25.278203354637252	23.3903237540924	24.840618884637546	26.4908540066328
13	32.88335771733849	16.1261029226288	23.61336438200487	27.377174978027842
14	24.96037045986198	19.035189969544334	20.803583519421796	35.20085605117189
15	29.6957354100894	24.39480315336964	18.86153690924105	27.047924527299905
16	28.93235230860326	22.986991951950674	21.13070977369342	26.94994596575264
17	27.50489228996344	23.683462865063078	21.19815301118124	27.613491833792246
18	27.873971424247173	20.017896355144405	23.780379328382185	28.327752892226236
19	27.597294835812892	23.196490827454177	21.73398156728525	27.47223276944768
20	28.5651318462188	20.09649162402785	22.6341098156463	28.704266714107053
21	31.313045487011866	21.176645522061108	21.59564327306811	25.914665717858913
22	30.686673056957677	20.47778488793536	20.436894106151406	28.398647948955556
23	27.728198442432948	20.720208808511657	21.92436267468197	29.62723007437343
24	26.941980229041484	22.49152312851654	22.523917124475258	28.042579517966722
25	27.391513304107928	21.11424725115702	21.928611067594588	29.565628377140456
26	28.783127507547533	22.299548873777592	21.63706510396614	27.280258514708734
27	29.200266586655268	20.435566483366213	21.841784537442948	28.522382392535572
28	27.26060969748787	21.40526216567139	23.024961963607204	28.30916617323353
29	25.746057624139368	21.711411979936965	24.478974437950892	28.063555957972774
30	24.884164911991885	23.762589183060594	24.588901604564896	26.76434430038262
31	28.069663022784663	22.82422539848598	21.505895972789045	27.600215605940313
32	26.168507194387875	21.70663253791027	23.363240249274455	28.761620018427404
33	25.79703833909079	20.78074840751647	24.120781810505747	29.301431442886994
34	27.633937224684225	21.267720445125367	23.428559290305966	27.669783039884443
35	26.984729682724705	20.888816902231202	24.66006218585126	27.466391229192833
36	24.59872601317533	22.38690645304331	24.905672401112017	28.108695132669343
37	26.634502791990716	20.06834602098175	25.493278245838567	27.803872941188967
38	24.600053635960524	18.985802401935143	27.43293513500596	28.981208827098374
39	25.546648681803337	18.261451410333684	28.02850671644367	28.163393191419306
40	23.879154463600567	19.018196397893856	28.293234699811208	28.809414438694365
41	22.170769463613844	19.73644032468343	30.612326180986848	27.48046403071588
42	23.410769144984375	19.295669559999258	30.162527581363364	27.131033713653007
43	20.590101775562715	19.218932963015085	30.888206195750012	29.30275906567219
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	7.5
2	12.0
3	34.0
4	56.0
5	56.0
6	78.0
7	100.0
8	90.5
9	81.0
10	124.5
11	168.0
12	168.0
13	289.0
14	410.0
15	648.0
16	886.0
17	801.0
18	716.0
19	716.0
20	798.0
21	880.0
22	712.5
23	545.0
24	631.5
25	718.0
26	718.0
27	882.0
28	1046.0
29	1499.0
30	1952.0
31	2445.5
32	2939.0
33	2939.0
34	3714.5
35	4490.0
36	5795.0
37	7100.0
38	9150.5
39	11201.0
40	11201.0
41	12743.5
42	14286.0
43	15850.0
44	17414.0
45	20271.5
46	23129.0
47	23129.0
48	27047.0
49	30965.0
50	31862.5
51	32760.0
52	33973.5
53	35187.0
54	35187.0
55	32550.0
56	29913.0
57	29526.5
58	29140.0
59	28678.0
60	28216.0
61	28216.0
62	27019.0
63	25822.0
64	24740.0
65	23658.0
66	20900.0
67	18142.0
68	18142.0
69	15487.0
70	12832.0
71	11052.5
72	9273.0
73	6826.5
74	4380.0
75	4380.0
76	3538.5
77	2697.0
78	2307.0
79	1917.0
80	1758.0
81	1599.0
82	1599.0
83	1226.5
84	854.0
85	710.0
86	566.0
87	452.0
88	338.0
89	338.0
90	255.0
91	172.0
92	103.0
93	34.0
94	23.5
95	13.0
96	13.0
97	8.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	376613.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.57012014827428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57751024599231	44.79375335574828
2	5.241546469836286	5.301312694847677
3	1.7193452255965298	2.608424839043348
4	0.8816770172876246	1.783460507848291
5	0.5853057314666874	1.4799490581871972
6	0.39436237684241543	1.196577166732798
7	0.28643406260137094	1.0139503472216753
8	0.23211091551148125	0.9390301508113238
9	0.1876783616751511	0.85418255692293
>10	1.5349098196037148	16.401742778250288
>50	0.20619064827061356	7.3530956092365765
>100	0.14712836539002858	14.191237182951596
>500	0.005273418114337942	1.7912720127386241
>1k	5.273418114337943E-4	0.2920117394594005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1095	0.2907493899573302	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	989	0.2626037869112325	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	808	0.21454384208723543	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	800	0.21241964563092616	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	750	0.19914341777899328	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	706	0.18746033726929234	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	565	0.15002137472684163	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	555	0.14736612915645503	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	530	0.14072801523048858	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	508	0.13488647497563813	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	506	0.13435542586156082	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	492	0.1306380820630196	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	480	0.12745178737855573	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	477	0.12665521370743973	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	464	0.12320339446593717	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	435	0.1155031823118161	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	434	0.11523765775477744	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	421	0.11178583851327491	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	409	0.108599543828811	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	406	0.10780297015769502	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	399	0.10594429825842443	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	397	0.10541324914434712	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	388	0.1030235281309992	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	385	0.10222695445988322	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC	378	0.10036828256061261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.655245570386577E-4	0.0
10	0.0	0.0	0.0	2.655245570386577E-4	0.0
11	0.0	0.0	0.0	5.310491140773154E-4	0.0
12	0.0	0.0	0.0	5.310491140773154E-4	0.0
13	0.0	0.0	0.0	5.310491140773154E-4	0.0
14	0.0	0.0	0.0	0.0010620982281546309	0.0
15	0.0	0.0	0.0	0.0010620982281546309	0.0
16	0.0	0.0	0.0	0.0021241964563092617	0.0
17	0.0	0.0	0.0	0.003717343798541208	0.0
18	0.0	0.0	0.0	0.004779442026695839	0.0
19	0.0	0.0	0.0	0.00584154025485047	0.0
20	0.0	0.0	0.0	0.006107064811889128	0.0
21	0.0	0.0	0.0	0.007434687597082416	0.0
22	0.0	0.0	0.0	0.01035545772450765	0.0
23	0.0	0.0	0.0	0.01619699797935812	0.0
24	0.0	0.0	0.0	0.02788007848905906	0.0
25	0.0	0.0	0.0	0.03239399595871624	0.0
26	0.0	0.0	0.0	0.04434260102545584	0.0
27	0.0	0.0	0.0	0.07886079344048134	0.0
28	0.0	0.0	0.0	0.2429549696903718	0.0
29	0.0	0.0	0.0	0.49122043052151676	0.0
30	0.0	0.0	0.0	0.8682653015164108	0.0
31	0.0	0.0	0.0	1.8222950349563078	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACT	45	1.3209059E-4	24.666666	4
CTTAGGA	70	1.915796E-7	23.785713	2
ATACACA	125	0.0	23.68	37
TATTGAC	55	1.8982584E-5	23.545454	30
TCAAAGC	120	0.0	23.125002	3
GATACAC	50	2.6974297E-4	22.2	3
GAATGTT	120	1.0913936E-11	21.583334	35
GTTCAAA	120	1.0913936E-11	21.583334	1
AATGTTT	120	1.0913936E-11	21.583334	36
AGTCGGT	525	0.0	21.495237	11
TTGCGCG	155	0.0	21.48387	18
TTTGCGC	155	0.0	21.48387	17
ATTTGCG	155	0.0	21.48387	16
GCAGTCG	510	0.0	21.40196	9
CAGTCGG	525	0.0	21.142857	10
TGCGCGC	160	0.0	20.8125	19
GATTCCT	535	0.0	20.747663	18
GCGAAAG	135	1.8189894E-12	20.555555	18
TCGGTGA	550	0.0	20.518183	13
TTAGGAC	100	1.2829332E-8	20.35	3
>>END_MODULE