FastQCFastQC Report
Fri 10 Feb 2017
ERR1631358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382659
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.3708262447766811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12770.33371748737126267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10700.2796223269281527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.16385345699434745No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3890.10165708894864618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCAAT400.001928846123.12500211
TGTTTAC502.6975406E-422.1999992
TAAGCCA502.6975406E-422.1999994
GGTATCA6250.021.0161
TTGTACT450.003821215420.5555554
TTTAGAC555.13448E-420.1818183
CACGGGT555.13448E-420.18181814
TCGCTTA555.13448E-420.18181830
ACCACGG555.13448E-420.18181812
TCTAGAC801.612082E-518.53
CCTATCG609.221473E-418.519
ATACACA1800.018.537
CCGACAT500.00702651918.49999814
TGACGGG752.0627286E-417.26666620
AGTCGGT1401.8553692E-917.17857211
GTTCTGC650.001577331517.0769231
ATTCGCT650.001577331517.07692328
GCCTATC650.001577331517.07692318
TATCGCT650.001577331517.07692321
ACCGCAG1201.0358235E-716.95833429
Produced by FastQC (version 0.11.5)