FastQCFastQC Report
Fri 10 Feb 2017
ERR1631357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141367
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1920.1358167040398396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1790.12662078137047542No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1490.10539942136425048No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1420.10044777069613134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAAAG352.3736262E-531.7142872
GGTATCA851.8189894E-1228.2941191
GTATCAA901.4006218E-1024.6666682
TCAAAGC451.3152084E-424.6666683
AAGCATT400.001922865223.12522
GTTCAAA400.001922865223.1251
AGATTAA502.6858912E-422.218
TATACAC753.6929669E-722.19999937
TTCGTCA450.003809478120.55555718
GAAAGCA450.003809478120.55555720
TATGCTT555.112488E-420.1818184
AAAGCAG555.112488E-420.1818185
CTTGTCT656.847556E-519.9230778
CAGAAGT500.00700514518.54
GTATTGT500.00700514518.56
GAATGTT500.00700514518.535
TTTCGTC500.00700514518.517
TTGTCTC701.2096255E-418.59
GTCTCAA609.1823E-418.511
GGTCCAA609.1823E-418.511
Produced by FastQC (version 0.11.5)