FastQCFastQC Report
Fri 10 Feb 2017
ERR1631356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609894
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104660.6501049137396624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81420.5057475833812661No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74880.46512379075889465No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC40140.24933318591161902No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT33290.20678380067259086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.19436062250061184No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA25680.1595136077282107No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21800.13541264207457138No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21430.13311435411275524No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC21000.13044337080577975No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA17890.11112532874835238No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG17790.11050416983975342No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17740.11019359038545394No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17520.10882704078653625No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG17080.10609394158870088No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC17050.1059075939161212No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC16620.10323661060914568No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT16560.10286391526398632No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT16510.10255333580968684No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT16140.10025504784787072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT358.8709476E-426.428579
ACCGTTA400.001931521723.1258
GGACCGT903.8307917E-922.611116
TGCATCA9750.020.49230814
TATAGAC656.904327E-519.9230773
GATACAC609.239006E-418.53
TTACGGC609.239006E-418.518
GACTGTA701.2195828E-418.57
GCATCAG11000.018.515
AGTCGGT13850.018.43321211
GCAGTCG13900.018.3669079
CAGTCGG14100.018.23758910
CTGCATC11250.017.92444413
GACAGGC11800.017.7161037
CAGGACA13450.017.4684034
TATACAC8500.017.41176637
GGTATCA45850.017.3097061
GCTGCAT11350.017.27753412
CCAGGAC13400.017.2574633
GGCAGTC15100.017.1523198
Produced by FastQC (version 0.11.5)