##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1609894
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03759750641968	34.0	33.0	34.0	31.0	34.0
2	33.174310234089944	34.0	33.0	34.0	31.0	34.0
3	33.240706530988994	34.0	33.0	34.0	31.0	34.0
4	36.47815756813802	37.0	37.0	37.0	35.0	37.0
5	36.47826627094703	37.0	37.0	37.0	35.0	37.0
6	36.53372271714784	37.0	37.0	37.0	35.0	37.0
7	36.53790560123834	37.0	37.0	37.0	35.0	37.0
8	36.53255866535312	37.0	37.0	37.0	35.0	37.0
9	38.37704532099629	39.0	39.0	39.0	37.0	39.0
10	38.341047298766256	39.0	39.0	39.0	37.0	39.0
11	38.39435826209676	39.0	39.0	39.0	37.0	39.0
12	38.36140764547231	39.0	39.0	39.0	37.0	39.0
13	38.37753293073954	39.0	39.0	39.0	37.0	39.0
14	39.89547945392678	41.0	40.0	41.0	38.0	41.0
15	39.88207484467922	41.0	40.0	41.0	38.0	41.0
16	39.84762909856177	41.0	40.0	41.0	38.0	41.0
17	39.84190077110667	41.0	40.0	41.0	38.0	41.0
18	39.79004207730447	41.0	40.0	41.0	38.0	41.0
19	39.814973532418904	41.0	40.0	41.0	38.0	41.0
20	39.791745916190756	41.0	40.0	41.0	38.0	41.0
21	39.76471494396525	41.0	40.0	41.0	38.0	41.0
22	39.69478052592282	41.0	40.0	41.0	37.0	41.0
23	39.620847086826835	41.0	40.0	41.0	37.0	41.0
24	39.58001893292354	41.0	40.0	41.0	37.0	41.0
25	39.56481234168212	41.0	40.0	41.0	37.0	41.0
26	39.44955320039705	41.0	39.0	41.0	36.0	41.0
27	39.389260410933886	41.0	39.0	41.0	36.0	41.0
28	39.28262233414125	41.0	39.0	41.0	35.0	41.0
29	39.201679737920635	41.0	39.0	41.0	35.0	41.0
30	39.104513092166314	41.0	39.0	41.0	35.0	41.0
31	39.03922618507802	41.0	39.0	41.0	35.0	41.0
32	38.93819158279986	41.0	38.0	41.0	35.0	41.0
33	38.82624446081543	41.0	38.0	41.0	35.0	41.0
34	38.745409325086	40.0	38.0	41.0	35.0	41.0
35	38.599477356894305	40.0	38.0	41.0	35.0	41.0
36	38.521703292266444	40.0	38.0	41.0	35.0	41.0
37	38.426360990226684	40.0	38.0	41.0	35.0	41.0
38	38.326057491983946	40.0	38.0	41.0	35.0	41.0
39	38.19898763521076	40.0	37.0	41.0	35.0	41.0
40	38.04629497345788	40.0	37.0	41.0	34.0	41.0
41	37.90787095299442	40.0	37.0	41.0	34.0	41.0
42	37.72618880497722	40.0	36.0	41.0	34.0	41.0
43	36.86397054712919	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	3.0
13	5.0
14	3.0
15	4.0
16	16.0
17	13.0
18	32.0
19	58.0
20	134.0
21	222.0
22	446.0
23	715.0
24	1132.0
25	1764.0
26	2553.0
27	3889.0
28	5334.0
29	7649.0
30	10231.0
31	12849.0
32	16066.0
33	21554.0
34	33482.0
35	52572.0
36	78818.0
37	159489.0
38	312554.0
39	888298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34223619691731	16.41940401045038	12.067129885570106	27.171229907062205
2	20.153128094147814	19.10777976686664	32.87495946938122	27.864132669604334
3	20.997593630388085	21.097972910017678	27.904942809899286	29.99949064969495
4	15.052295368514946	14.932722278609647	31.575743496155646	38.43923885671976
5	16.824399618856894	34.88993685298535	30.70407119971874	17.581592328439015
6	35.81453188843489	31.996827120294874	15.840918718872175	16.34772227239806
7	31.682085901307786	28.708163394608587	19.21219657940212	20.3975541246815
8	30.612760840154696	29.859108736351587	18.411025819091194	21.117104604402527
9	28.893517212934515	13.304105736154057	18.06398433685696	39.73839271405446
10	20.489050831918128	24.308929656238238	27.86742481181991	27.33459470002373
11	38.26879285220021	21.041571681116896	18.669986968086096	22.0196484985968
12	25.358067052861866	24.38297179814323	25.159047738546764	25.099913410448142
13	31.87507997420948	17.007144569766705	23.88076482054098	27.237010635482832
14	24.34334185977462	19.494699651032928	22.884674394711702	33.277284094480756
15	28.367768312696363	24.759456212645055	20.387615582143916	26.485159892514666
16	27.12557472728018	23.664912099802844	21.955793362792832	27.253719810124146
17	25.725171967843846	24.651809373784857	23.256934928635054	26.366083729736246
18	26.081406601925345	21.39848958999785	25.346451381271063	27.173652426805738
19	26.341858532300883	23.628698535431525	24.227495723320914	25.801947208946675
20	26.543735177595547	22.202641913070053	24.568636195923457	26.684986713410947
21	29.716180071482967	22.153135548054717	23.61503303944235	24.515651341019968
22	28.57175689828026	22.245688225435963	22.98082979376282	26.20172508252096
23	26.40341538014304	22.540366011675303	23.78088246803827	27.275336140143384
24	26.23234821671489	23.34122619253193	24.263398708237936	26.163026882515243
25	26.03065791909281	22.3410361179059	24.13407342346763	27.49423253953366
26	26.963949179262737	23.47260130170061	24.348373246934273	25.215076272102387
27	27.748410764932352	22.074248366662648	23.416200072799825	26.761140795605176
28	25.340053444512495	22.528750340084503	25.294274032948756	26.83692218245425
29	25.132772716713024	22.751125229362927	25.804059149235915	26.31204290468813
30	24.370175924626096	23.610125884064416	26.253281271934675	25.76641691937482
31	25.856298613449084	23.515647614066516	23.954247919428234	26.673805853056166
32	24.805297740099657	22.92206816100936	25.103454016227154	27.16918008266383
33	25.14115836197911	22.582232122114874	25.387696332802033	26.88891318310398
34	26.246262176267503	22.959337695525296	24.808776229987814	25.985623898219384
35	26.31452754032253	22.674598451823535	25.580441942140293	25.430432065713642
36	24.424278865565064	23.640127859349747	24.90722991700075	27.028363358084444
37	24.710819470101757	22.45831091984938	25.963448525182404	26.867421084866454
38	24.60839036607379	21.14971544710397	27.22943249679793	27.012461690024313
39	24.297127636974857	21.230838800566996	27.368137281088075	27.103896281370076
40	23.98101986838885	21.170959081778054	28.139865109131406	26.708155940701683
41	22.202952492524354	21.675961274469003	29.52039078349258	26.600695449514067
42	23.07959406023005	22.041389060397766	28.659775115628733	26.21924176374345
43	21.593160791952762	20.851000127958734	29.62033525188615	27.93550382820235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	245.5
2	229.0
3	674.0
4	1119.0
5	1119.0
6	1501.5
7	1884.0
8	1838.0
9	1792.0
10	2773.5
11	3755.0
12	3755.0
13	6553.5
14	9352.0
15	14706.0
16	20060.0
17	17656.0
18	15252.0
19	15252.0
20	16525.5
21	17799.0
22	13175.5
23	8552.0
24	8637.0
25	8722.0
26	8722.0
27	9509.5
28	10297.0
29	12266.0
30	14235.0
31	17153.5
32	20072.0
33	20072.0
34	25314.0
35	30556.0
36	37395.0
37	44234.0
38	50575.0
39	56916.0
40	56916.0
41	61564.0
42	66212.0
43	71347.5
44	76483.0
45	83061.0
46	89639.0
47	89639.0
48	99756.0
49	109873.0
50	114326.5
51	118780.0
52	122173.0
53	125566.0
54	125566.0
55	123258.0
56	120950.0
57	116389.5
58	111829.0
59	107205.0
60	102581.0
61	102581.0
62	98748.5
63	94916.0
64	88143.5
65	81371.0
66	72626.5
67	63882.0
68	63882.0
69	55425.5
70	46969.0
71	42949.0
72	38929.0
73	35695.0
74	32461.0
75	32461.0
76	30494.5
77	28528.0
78	23039.5
79	17551.0
80	12285.5
81	7020.0
82	7020.0
83	5486.0
84	3952.0
85	3620.0
86	3288.0
87	2983.0
88	2678.0
89	2678.0
90	1875.0
91	1072.0
92	625.5
93	179.0
94	122.5
95	66.0
96	66.0
97	45.0
98	24.0
99	15.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1609894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.047643812220755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23506007324865	36.6802873389764
2	6.442519612569211	5.417855398451134
3	1.9097949454994152	2.4090713286821686
4	0.9692663729316561	1.6302146883277362
5	0.6115944984602025	1.2858053814384196
6	0.41224585968720007	1.0400380242714073
7	0.29247967135456276	0.8608656730402428
8	0.2133299988302178	0.7176019044219576
9	0.17141780764432105	0.6486943427010167
>10	1.3141101548341378	11.137138399994651
>50	0.19316117620032003	5.68923557736887
>100	0.19898840944524526	17.892893744586395
>500	0.02683821766418007	7.615021265864135
>1k	0.008748369293848752	5.348265126031466
>5k	2.965548913169068E-4	0.9744863015536105
>10k+	1.482774456584534E-4	0.6525255042904726
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10466	0.6501049137396624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8142	0.5057475833812661	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7488	0.46512379075889465	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4014	0.24933318591161902	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3329	0.20678380067259086	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3129	0.19436062250061184	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2568	0.1595136077282107	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2180	0.13541264207457138	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2143	0.13311435411275524	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2100	0.13044337080577975	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1789	0.11112532874835238	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1779	0.11050416983975342	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1774	0.11019359038545394	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1752	0.10882704078653625	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1708	0.10609394158870088	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1705	0.1059075939161212	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1662	0.10323661060914568	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1656	0.10286391526398632	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1651	0.10255333580968684	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1614	0.10025504784787072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	6.211589085989513E-5	0.0
7	0.0	0.0	0.0	6.211589085989513E-5	0.0
8	0.0	0.0	0.0	6.211589085989513E-5	0.0
9	0.0	0.0	0.0	6.211589085989513E-5	0.0
10	0.0	6.211589085989513E-5	0.0	1.8634767257968537E-4	6.211589085989513E-5
11	0.0	6.211589085989513E-5	0.0	6.211589085989512E-4	6.211589085989513E-5
12	0.0	6.211589085989513E-5	0.0	0.0011180860354781123	6.211589085989513E-5
13	0.0	6.211589085989513E-5	0.0	0.0014286654897775878	6.211589085989513E-5
14	0.0	6.211589085989513E-5	0.0	0.001987708507516644	6.211589085989513E-5
15	0.0	6.211589085989513E-5	0.0	0.003105794542994756	6.211589085989513E-5
16	0.0	6.211589085989513E-5	0.0	0.0062737049768494075	6.211589085989513E-5
17	0.0	6.211589085989513E-5	0.0	0.009006804174684793	6.211589085989513E-5
18	0.0	6.211589085989513E-5	0.0	0.01006277431930301	1.2423178171979026E-4
19	0.0	6.211589085989513E-5	0.0	0.01211259871767955	1.2423178171979026E-4
20	0.0	6.211589085989513E-5	0.0	0.013913959552616508	1.2423178171979026E-4
21	0.0	6.211589085989513E-5	0.0	0.018013608349369586	1.2423178171979026E-4
22	0.0	6.211589085989513E-5	0.0	0.024660008671378363	1.2423178171979026E-4
23	0.0	6.211589085989513E-5	0.0	0.031927567901986094	1.2423178171979026E-4
24	0.0	6.211589085989513E-5	0.0	0.04273573291160784	1.2423178171979026E-4
25	0.0	6.211589085989513E-5	0.0	0.04733230883524008	1.8634767257968537E-4
26	0.0	6.211589085989513E-5	0.0	0.05807835795400194	1.8634767257968537E-4
27	0.0	1.2423178171979026E-4	0.0	0.09640386261455723	2.484635634395805E-4
28	0.0	1.2423178171979026E-4	0.0	0.23809020966597802	2.484635634395805E-4
29	0.0	1.2423178171979026E-4	0.0	0.43524604725528515	2.484635634395805E-4
30	0.0	1.2423178171979026E-4	0.0	0.7141463972162142	2.484635634395805E-4
31	0.0	1.2423178171979026E-4	0.0	1.3715188701864842	2.484635634395805E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	35	8.8709476E-4	26.42857	9
ACCGTTA	40	0.0019315217	23.125	8
GGACCGT	90	3.8307917E-9	22.61111	6
TGCATCA	975	0.0	20.492308	14
TATAGAC	65	6.904327E-5	19.923077	3
GATACAC	60	9.239006E-4	18.5	3
TTACGGC	60	9.239006E-4	18.5	18
GACTGTA	70	1.2195828E-4	18.5	7
GCATCAG	1100	0.0	18.5	15
AGTCGGT	1385	0.0	18.433212	11
GCAGTCG	1390	0.0	18.366907	9
CAGTCGG	1410	0.0	18.237589	10
CTGCATC	1125	0.0	17.924444	13
GACAGGC	1180	0.0	17.716103	7
CAGGACA	1345	0.0	17.468403	4
TATACAC	850	0.0	17.411766	37
GGTATCA	4585	0.0	17.309706	1
GCTGCAT	1135	0.0	17.277534	12
CCAGGAC	1340	0.0	17.257463	3
GGCAGTC	1510	0.0	17.152319	8
>>END_MODULE