FastQCFastQC Report
Fri 10 Feb 2017
ERR1631355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789705
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.1994415636218588No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14370.18196668376165784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14280.18082701768381865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11430.14473759188557753No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11330.143471296243534No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10570.13384744936400303No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10270.13004856243787238No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9480.12004482686572834No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT9470.11991819730152399No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC9000.11396660778391932No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8840.11194053475664964No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG8240.10434276090438835No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8040.10181016962030126No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7920.100290614849849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC400.001930655223.1251
GGTATCA8700.021.2643681
GTGCAAC450.003824763720.5555551
GTTTAGG701.2185843E-418.51
TTAACGG609.2333247E-418.535
AGTACCG801.6156271E-518.55
GTAATAC500.00703298118.4999983
GTAAGTG500.00703298118.4999983
TCTAGCG3250.015.93846128
CTTATAC10150.015.85714337
AGGACCG3300.015.696975
TACGCCT957.0552036E-515.57894835
AAGACGG2900.015.3103455
ATAGGAC3150.015.2698423
CTAGCGG3300.015.13636429
CGCAATA3450.015.01449236
CGGTCCA3600.014.90277810
ATTGCCG1252.9557232E-614.80000111
CATATAC750.00410289414.83
CGCGGTT3000.014.810
Produced by FastQC (version 0.11.5)