##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789705
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06780506644886	34.0	33.0	34.0	31.0	34.0
2	33.20441810549509	34.0	33.0	34.0	31.0	34.0
3	33.28764918545533	34.0	34.0	34.0	31.0	34.0
4	36.51813525303753	37.0	37.0	37.0	35.0	37.0
5	36.50710455169968	37.0	37.0	37.0	35.0	37.0
6	36.55718781063815	37.0	37.0	37.0	35.0	37.0
7	36.559267068082384	37.0	37.0	37.0	35.0	37.0
8	36.554584306798105	37.0	37.0	37.0	35.0	37.0
9	38.40828283979461	39.0	39.0	39.0	37.0	39.0
10	38.37261635674081	39.0	39.0	39.0	37.0	39.0
11	38.426003381009366	39.0	39.0	39.0	37.0	39.0
12	38.39030903945144	39.0	39.0	39.0	37.0	39.0
13	38.41115479831076	39.0	39.0	39.0	37.0	39.0
14	39.96785761771801	41.0	40.0	41.0	38.0	41.0
15	39.96034975085633	41.0	40.0	41.0	38.0	41.0
16	39.922260844239304	41.0	40.0	41.0	38.0	41.0
17	39.91720452574062	41.0	40.0	41.0	38.0	41.0
18	39.887807472410586	41.0	40.0	41.0	38.0	41.0
19	39.907624999208565	41.0	40.0	41.0	38.0	41.0
20	39.87584984266277	41.0	40.0	41.0	38.0	41.0
21	39.84568794676493	41.0	40.0	41.0	38.0	41.0
22	39.78617964936274	41.0	40.0	41.0	38.0	41.0
23	39.71962694930385	41.0	40.0	41.0	38.0	41.0
24	39.68905097473107	41.0	40.0	41.0	37.0	41.0
25	39.68492411723365	41.0	40.0	41.0	37.0	41.0
26	39.59525772282055	41.0	40.0	41.0	37.0	41.0
27	39.54752850748064	41.0	40.0	41.0	37.0	41.0
28	39.46192945466978	41.0	40.0	41.0	36.0	41.0
29	39.401795607220414	41.0	39.0	41.0	36.0	41.0
30	39.339645817108924	41.0	39.0	41.0	36.0	41.0
31	39.30210015132233	41.0	39.0	41.0	35.0	41.0
32	39.23253366763538	41.0	39.0	41.0	35.0	41.0
33	39.15093104387081	41.0	39.0	41.0	35.0	41.0
34	39.10362477127535	41.0	39.0	41.0	35.0	41.0
35	39.0026022375444	41.0	39.0	41.0	35.0	41.0
36	38.94066518510076	41.0	39.0	41.0	35.0	41.0
37	38.87909282580204	41.0	39.0	41.0	35.0	41.0
38	38.79997973926973	40.0	38.0	41.0	35.0	41.0
39	38.71208489245984	40.0	38.0	41.0	35.0	41.0
40	38.61355189596115	40.0	38.0	41.0	35.0	41.0
41	38.52309026788485	40.0	38.0	41.0	35.0	41.0
42	38.393879993162	40.0	37.0	41.0	35.0	41.0
43	37.60073825035931	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	0.0
15	3.0
16	4.0
17	8.0
18	10.0
19	40.0
20	49.0
21	126.0
22	177.0
23	291.0
24	445.0
25	693.0
26	1007.0
27	1575.0
28	2049.0
29	2907.0
30	3824.0
31	4829.0
32	6635.0
33	8898.0
34	13452.0
35	19959.0
36	33401.0
37	65056.0
38	148846.0
39	475418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.224875111592304	17.96797538321272	12.15352568364136	26.65362382155362
2	19.611880385713654	19.76814126794183	33.16833501117506	27.45164333516946
3	20.2384434693968	21.38760676455132	28.7989818983038	29.574967867748082
4	14.805528646773162	15.471853413616476	33.26609303474083	36.45652490486954
5	16.493627367181414	34.48946125451909	32.292311686009334	16.72459969229016
6	35.01649350073762	33.68435048530781	15.678132973705372	15.621023040249208
7	30.509620681140426	29.324494589751872	20.33176945821541	19.834115270892294
8	28.591182783444452	31.05400117765495	19.55945574613305	20.795360292767555
9	27.320328477089546	14.021185126091387	19.31113517072831	39.34735122609075
10	19.169436688383637	24.587789111123772	30.20735591138463	26.03541828910796
11	37.974560120551345	20.746101392292058	20.024566135455647	21.25477235170095
12	23.448502921977195	24.630589903824845	26.649318416370672	25.27158875782729
13	30.88254474772225	17.71243692264833	24.462679101689872	26.94233922793955
14	24.14129326773922	19.947575360419396	22.981872977884148	32.92925839395724
15	27.128611316884154	25.96868450877226	20.762309976510217	26.140394197833366
16	27.093281668471136	24.449129738320003	22.55386505087343	25.903723542335428
17	25.943105336802986	24.811416921508663	23.239184252347396	26.00629348934096
18	26.00819293280402	22.718356854774886	25.080504745442916	26.192945466978173
19	26.15546311597369	23.9371664102418	24.297047631710576	25.61032284207394
20	26.6239925035298	22.67428976643177	24.091401219442705	26.610316510595727
21	28.122653395888335	23.615147428470127	23.376450699944918	24.88574847569662
22	27.753781475361052	22.947809625113173	23.303512070963208	25.994896828562563
23	26.12849101879816	22.807757327103158	24.189159243008465	26.874592411090216
24	25.72314978378002	24.113561393178465	23.915512754762855	26.24777606827866
25	26.08632337391811	22.939958592132506	23.99794860105989	26.975769432889496
26	25.87447211300422	24.51902925776081	24.121918944415953	25.484579684819014
27	27.060104722649598	22.882722029112138	23.650603706447342	26.40656954179092
28	25.494203531698545	23.53765013517706	24.79356215295585	26.17458418016854
29	25.14014727018317	23.7424101404955	25.042642505745818	26.07480008357551
30	24.522448255994327	24.705301346705415	25.12444520422183	25.647805193078426
31	26.162807630697536	23.902723168778213	23.535117543892973	26.399351656631275
32	25.20232238620751	23.27451390076041	24.081650742998967	27.441512970033116
33	24.620586168252704	23.026953102740897	25.007312857332803	27.345147871673596
34	26.217891491126434	22.774453751717413	24.929562304911325	26.078092452244828
35	25.972103507005777	22.773314085639573	25.751261547033387	25.50332086032126
36	24.3821426988559	23.801799406107342	25.07936507936508	26.736692815671674
37	25.351745271968646	22.179801318213762	25.927783159534258	26.540670250283334
38	24.36403467117468	21.7882627056939	26.837363319214134	27.010339303917284
39	24.7950817077263	21.47979308729209	26.86180282510558	26.86332237987603
40	24.032518472087677	21.937052443634013	27.770116689143414	26.260312395134893
41	22.887913841244515	22.181574132112626	28.84102291361964	26.089489113023216
42	23.957047251821884	22.20854622928815	28.401111807573713	25.43329471131625
43	22.259831202790917	21.28984874098556	28.720598198061303	27.729721858162225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	91.5
2	77.0
3	194.0
4	311.0
5	311.0
6	371.0
7	431.0
8	409.0
9	387.0
10	599.0
11	811.0
12	811.0
13	1286.5
14	1762.0
15	2657.5
16	3553.0
17	3358.5
18	3164.0
19	3164.0
20	3659.5
21	4155.0
22	3998.0
23	3841.0
24	4478.5
25	5116.0
26	5116.0
27	5994.5
28	6873.0
29	8593.5
30	10314.0
31	12186.0
32	14058.0
33	14058.0
34	16939.0
35	19820.0
36	23674.5
37	27529.0
38	31171.5
39	34814.0
40	34814.0
41	37686.0
42	40558.0
43	42299.5
44	44041.0
45	46686.0
46	49331.0
47	49331.0
48	52306.0
49	55281.0
50	56569.0
51	57857.0
52	61110.0
53	64363.0
54	64363.0
55	61769.5
56	59176.0
57	57166.0
58	55156.0
59	52906.5
60	50657.0
61	50657.0
62	47641.0
63	44625.0
64	41347.5
65	38070.0
66	33865.0
67	29660.0
68	29660.0
69	25496.5
70	21333.0
71	18791.5
72	16250.0
73	12499.0
74	8748.0
75	8748.0
76	7134.5
77	5521.0
78	4697.5
79	3874.0
80	3370.5
81	2867.0
82	2867.0
83	2369.0
84	1871.0
85	1690.5
86	1510.0
87	1368.5
88	1227.0
89	1227.0
90	851.5
91	476.0
92	285.0
93	94.0
94	59.0
95	24.0
96	24.0
97	17.5
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	789705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.733266353818856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19535765941723	48.819532682970205
2	5.689781694191468	6.228406739265287
3	1.59937742089295	2.6261745113405306
4	0.8187401527253909	1.7924929141474064
5	0.47088137385048795	1.2886437828005457
6	0.3114851696595282	1.0229160453743649
7	0.2350359926118109	0.9005001310449514
8	0.16725201954215818	0.732339946705205
9	0.1412747715836383	0.6959186731945975
>10	1.0858695125113587	12.103909394330698
>50	0.15000742587321986	5.726462967712475
>100	0.1235761481204935	13.490271000641343
>500	0.009737707731690819	3.455718437044479
>1k	0.0016229512886151366	1.1167127734280045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1575	0.1994415636218588	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1437	0.18196668376165784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.18082701768381865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1143	0.14473759188557753	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1133	0.143471296243534	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1057	0.13384744936400303	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1027	0.13004856243787238	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	948	0.12004482686572834	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	947	0.11991819730152399	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	900	0.11396660778391932	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	884	0.11194053475664964	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	824	0.10434276090438835	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	804	0.10181016962030126	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	792	0.100290614849849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.532591284087096E-4	0.0
15	0.0	0.0	0.0	3.798886926130644E-4	0.0
16	0.0	0.0	0.0	6.331478210217739E-4	0.0
17	0.0	0.0	0.0	0.0010130365136348384	0.0
18	0.0	0.0	0.0	0.001139666077839193	0.0
19	0.0	0.0	0.0	0.0013929252062479026	0.0
20	0.0	0.0	0.0	0.0021527025914740315	0.0
21	0.0	0.0	0.0	0.002405961719882741	0.0
22	0.0	0.0	0.0	0.0034189982335175793	0.0
23	0.0	0.0	0.0	0.004685293875561127	0.0
24	0.0	0.0	0.0	0.007471144288056932	0.0
25	0.0	0.0	0.0	0.009750476443735319	0.0
26	0.0	0.0	0.0	0.01874117550224451	0.0
27	0.0	0.0	0.0	0.05571700824991611	0.0
28	0.0	0.0	0.0	0.19640245408095428	0.0
29	0.0	0.0	0.0	0.3812816178193123	0.0
30	0.0	0.0	0.0	0.631754895815526	0.0
31	0.0	0.0	0.0	1.2262806997549718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCTAC	40	0.0019306552	23.125	1
GGTATCA	870	0.0	21.264368	1
GTGCAAC	45	0.0038247637	20.555555	1
GTTTAGG	70	1.2185843E-4	18.5	1
TTAACGG	60	9.2333247E-4	18.5	35
AGTACCG	80	1.6156271E-5	18.5	5
GTAATAC	50	0.007032981	18.499998	3
GTAAGTG	50	0.007032981	18.499998	3
TCTAGCG	325	0.0	15.938461	28
CTTATAC	1015	0.0	15.857143	37
AGGACCG	330	0.0	15.69697	5
TACGCCT	95	7.0552036E-5	15.578948	35
AAGACGG	290	0.0	15.310345	5
ATAGGAC	315	0.0	15.269842	3
CTAGCGG	330	0.0	15.136364	29
CGCAATA	345	0.0	15.014492	36
CGGTCCA	360	0.0	14.902778	10
ATTGCCG	125	2.9557232E-6	14.800001	11
CATATAC	75	0.004102894	14.8	3
CGCGGTT	300	0.0	14.8	10
>>END_MODULE