FastQCFastQC Report
Fri 10 Feb 2017
ERR1631354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552016
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.4960001159386685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21560.3905683893220486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20260.36701834729428134No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14280.2586881539665517No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12110.21937769919712471No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9910.17952378191936466No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9860.17861801107214284No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9770.17698762354714356No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8280.14999565229993334No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8010.14510448972493553No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT7680.13912640213327152No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7540.13659024376105042No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC7410.13423523955827368No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7340.13296716037216313No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7330.13278600620271877No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC7090.12843830613605403No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC7060.12789484362772094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.12698907278049912No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG6550.11865598098605838No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC6520.11811251847772529No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA6280.11376481841106055No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC6260.11340251007217182No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG6140.11122866003883945No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT6100.110504043361062No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC6080.11014173502217327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA5890.10669980580273036No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5880.106518651633286No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG5870.10633749746384163No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT5550.10054056404162198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGA451.3219951E-424.6666681
TTATACC400.001929922723.1254
CATACAC502.699638E-422.1999993
TAGTACC502.699638E-422.1999994
TAATAGA450.003823326420.5555574
GCATTAT555.138441E-420.1818181
GGTATCA11750.019.5234051
TCGTTTA1501.2732926E-1118.530
TTAGAAC701.2177415E-418.53
ATAGTAC609.228526E-418.53
CGCTTAT801.6141912E-518.526
ATACACT500.00703036518.4999984
TATACAC2050.018.0487837
CGTTTAT1552.0008883E-1117.90322531
GAATAAC1900.017.5263161
GCTTATT852.7189668E-517.41176427
TACTGGA852.7189668E-517.4117642
CTTAGAA852.7189668E-517.4117642
AAGACGG2450.017.3673465
TAACGCC1603.45608E-1117.343754
Produced by FastQC (version 0.11.5)