FastQCFastQC Report
Fri 10 Feb 2017
ERR1631353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96266
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22382.3248083435480855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17841.8531984293520039No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14891.5467558639602768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.6160014958552344No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5010.520432966987306No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2710.28151164481748486No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2210.22957222695448026No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT2190.2274946502399601No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.21295161323831882No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT2010.20879645980927844No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1990.20671888309475828No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.20464130638023809No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.20256372966571792No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1860.1932146344503771No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1820.1890594810213367No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1810.18802069266407662No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.17451644401969543No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1670.17347765566243534No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1650.17140007894791515No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1590.1651673488043546No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1450.15062431180271332No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1400.14543037001641285No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1340.1391976398728523No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1260.13088733301477157No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1288097563002514No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.12465460287121102No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.12153823779943074No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1170.12153823779943074No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1100.1142667192986101No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT1040.10803398915504955No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA990.1028400473687491No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG990.1028400473687491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGA358.8102417E-426.428571
ATGCTGT502.6772596E-422.237
ATACACA603.6808357E-521.58333437
GGGCAGT4400.021.4431827
GCAGTCG4450.021.2022489
AGTCGGT4300.021.08139411
CAGTCGG4500.020.96666710
GGCAGTC4550.020.7362638
TGGGCAG4550.020.7362636
TGACTGT555.0961954E-420.18181833
CTCTATG6650.019.1954881
GGTGATT4600.018.90217415
GTCGGTG4750.018.69473612
ATTCCTC4800.018.519
GATTCCT4700.018.518
TCGGTGA4700.018.513
GTGATTC4750.018.30526416
TCTATGG7100.018.2394372
CGGTGAT4900.018.12244814
TGATTCC4800.018.11458417
Produced by FastQC (version 0.11.5)