##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96266
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81093013109509	34.0	31.0	34.0	31.0	34.0
2	32.90248893690399	34.0	31.0	34.0	31.0	34.0
3	32.89962188103796	34.0	33.0	34.0	31.0	34.0
4	36.292263104315126	37.0	37.0	37.0	35.0	37.0
5	36.296252051607006	37.0	37.0	37.0	35.0	37.0
6	36.391685537988494	37.0	37.0	37.0	35.0	37.0
7	36.39224648370141	37.0	37.0	37.0	35.0	37.0
8	36.412502856667984	37.0	37.0	37.0	35.0	37.0
9	38.194928635239854	39.0	39.0	39.0	37.0	39.0
10	38.150634699686286	39.0	38.0	39.0	37.0	39.0
11	38.223484927180934	39.0	39.0	39.0	37.0	39.0
12	38.177819790995784	39.0	38.0	39.0	37.0	39.0
13	38.20410113643446	39.0	39.0	39.0	37.0	39.0
14	39.550672096067146	41.0	39.0	41.0	37.0	41.0
15	39.5114889992313	41.0	39.0	41.0	37.0	41.0
16	39.38763426339518	40.0	39.0	41.0	37.0	41.0
17	39.433195520744604	40.0	39.0	41.0	37.0	41.0
18	39.55183553902728	41.0	39.0	41.0	37.0	41.0
19	39.59763571769888	41.0	40.0	41.0	37.0	41.0
20	39.59488292855214	41.0	40.0	41.0	37.0	41.0
21	39.58768412523632	41.0	40.0	41.0	37.0	41.0
22	39.509733446907525	41.0	39.0	41.0	37.0	41.0
23	39.430297301227846	41.0	39.0	41.0	36.0	41.0
24	39.36790767249081	41.0	39.0	41.0	36.0	41.0
25	39.31572933330563	41.0	39.0	41.0	35.0	41.0
26	39.10628882471485	41.0	39.0	41.0	35.0	41.0
27	38.978299711216835	40.0	38.0	41.0	35.0	41.0
28	38.84090956308562	40.0	38.0	41.0	35.0	41.0
29	38.646074418797916	40.0	38.0	41.0	35.0	41.0
30	38.386481208318614	40.0	37.0	41.0	35.0	41.0
31	38.11951260050277	40.0	37.0	41.0	35.0	41.0
32	37.873880705545055	40.0	35.0	41.0	35.0	41.0
33	37.65852949120146	40.0	35.0	41.0	34.0	41.0
34	37.471828059751104	40.0	35.0	41.0	34.0	41.0
35	37.28268547566119	40.0	35.0	41.0	33.0	41.0
36	37.05580371055201	39.0	35.0	41.0	33.0	41.0
37	36.83168512247315	39.0	35.0	41.0	33.0	41.0
38	36.573816300666905	39.0	35.0	41.0	33.0	41.0
39	36.25952049529429	39.0	35.0	41.0	31.0	41.0
40	35.982454864645874	39.0	35.0	41.0	31.0	41.0
41	35.62080069806578	39.0	35.0	41.0	29.0	41.0
42	35.270853676272	38.0	35.0	41.0	26.0	41.0
43	34.28408783994349	37.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	3.0
18	6.0
19	4.0
20	10.0
21	42.0
22	46.0
23	72.0
24	140.0
25	192.0
26	301.0
27	458.0
28	669.0
29	943.0
30	1114.0
31	1359.0
32	1625.0
33	2010.0
34	2841.0
35	4008.0
36	6766.0
37	16305.0
38	22427.0
39	34918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.50032202439075	14.762221345023166	15.50495502046413	23.232501610121954
2	20.254295389857273	21.163235202459852	32.6148380528951	25.967631354787773
3	21.115450938025887	23.449608376789314	29.76648037728793	25.66846030789687
4	14.386179959695012	17.88793551201878	29.88074709658654	37.84513743169967
5	14.613674609934971	38.34894978497081	29.176448590364203	17.86092701473002
6	30.054224752248977	30.458313423223153	19.291338582677163	20.196123241850707
7	29.506783287972908	31.932354102175225	17.664596015207863	20.896266594644008
8	34.85031059771882	27.985997132944135	16.98315085284524	20.180541416491803
9	30.968358506637855	11.833876965906965	17.212723079799723	39.985041447655455
10	22.231109633723225	26.508840088920284	22.43263457503168	28.82741570232481
11	34.55113955082791	24.53929736355515	15.663889639124925	25.245673446492013
12	24.191303263873017	30.21731452433881	23.7726715558972	21.818710655890968
13	35.931689277626575	17.991814347744793	24.171566285085078	21.904930089543555
14	24.594353146489933	20.978330874867552	25.01610121953753	29.411214759104983
15	32.16296511748696	23.398707747283567	20.93781812893441	23.500509006295054
16	23.734236386678578	24.716930172646627	25.751563376477677	25.79727006419712
17	20.36129059065506	29.370702013171833	25.398375335009245	24.869632061163855
18	22.674672262273283	21.650426942014832	29.097500675212434	26.57740012049945
19	23.315604678702762	27.220410113643446	29.905678017160785	19.55830719049301
20	25.45135354122951	21.752228201026323	31.33920595017971	21.457212307564458
21	28.53032223214842	20.959632684436873	28.67263623709305	21.83740884632165
22	27.14769492863524	22.034778634201068	26.097479899445286	24.720046537718403
23	23.87447281490869	24.170527496727818	26.893191781106516	25.061807907256977
24	22.49807824153907	24.599547088276235	29.90879438223256	22.993580287952135
25	22.089834417135854	24.10300625350591	27.788627345064715	26.01853198429352
26	25.20516070055887	22.637275881411924	27.854071011572103	24.30349240645711
27	23.590883593376685	23.361311366422203	27.069785801840734	25.97801923836038
28	21.914279184758897	24.002243782851682	29.8921737685164	24.191303263873017
29	22.261234496083766	24.366858496249975	31.288305320673963	22.083601686992292
30	21.46863897949432	24.972472108532607	32.384227037583365	21.17466187438971
31	22.054515612989007	26.136953857021172	29.124509172501195	22.684021357488625
32	20.912887208360168	25.79727006419712	32.14218935034176	21.14765337710095
33	21.59952631250909	24.717968961003887	31.864832858953317	21.817671867533708
34	22.32148422080485	25.31423347807118	28.29867242847942	24.065609872644547
35	20.57528099225064	26.481831591631522	29.292792886377327	23.65009452974051
36	18.937111752851475	25.60405542974674	30.46662373008123	24.99220908732055
37	19.34016163546839	25.511603265950594	30.872789977769926	24.27544512081109
38	20.033033469760873	22.541707352543995	32.75403569276796	24.671223484927182
39	20.499449442170654	22.3827727338832	33.469760870920155	23.64801695302599
40	19.587393264496292	22.30694118380321	32.09024993247876	26.015415619221738
41	16.64138948330667	23.31041073691646	34.54179045561258	25.5064093241643
42	16.756694990962544	22.788938981571892	33.81879375895955	26.635572268506014
43	15.430162258741403	24.734589574720047	35.06118463424262	24.77406353229593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	14.5
2	27.0
3	106.5
4	186.0
5	186.0
6	264.0
7	342.0
8	366.0
9	390.0
10	636.5
11	883.0
12	883.0
13	1459.0
14	2035.0
15	3188.5
16	4342.0
17	3723.0
18	3104.0
19	3104.0
20	3421.0
21	3738.0
22	2589.0
23	1440.0
24	1265.5
25	1091.0
26	1091.0
27	972.5
28	854.0
29	825.0
30	796.0
31	803.5
32	811.0
33	811.0
34	832.0
35	853.0
36	939.0
37	1025.0
38	1248.0
39	1471.0
40	1471.0
41	1890.0
42	2309.0
43	2955.0
44	3601.0
45	4858.5
46	6116.0
47	6116.0
48	7871.5
49	9627.0
50	9089.5
51	8552.0
52	7242.5
53	5933.0
54	5933.0
55	5267.0
56	4601.0
57	4637.5
58	4674.0
59	4638.0
60	4602.0
61	4602.0
62	4625.5
63	4649.0
64	4527.5
65	4406.0
66	4222.0
67	4038.0
68	4038.0
69	3699.0
70	3360.0
71	2958.0
72	2556.0
73	2139.0
74	1722.0
75	1722.0
76	1412.5
77	1103.0
78	830.0
79	557.0
80	426.5
81	296.0
82	296.0
83	211.5
84	127.0
85	82.0
86	37.0
87	22.5
88	8.0
89	8.0
90	4.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	96266.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.159391685538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.04711959679067	60.23622047244095
2	4.969460974422585	6.575530301456381
3	1.3047779051327546	2.589699374649409
4	0.6892870040352338	1.8241123553487213
5	0.39724285198385906	1.314067271934016
6	0.2559311655073875	1.0159350134003697
7	0.20568701031575312	0.9525689236075042
8	0.15073246557490302	0.7977894583757505
9	0.12718051782882445	0.757276712442607
>10	0.7285402502786981	9.220285459040575
>50	0.0769363626371901	3.541229509899653
>100	0.03925324624346433	4.314088047701161
>500	0.003140259699477147	1.1364344628425405
>1k	0.0047103895492157195	5.7247626368603655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2238	2.3248083435480855	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1784	1.8531984293520039	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1489	1.5467558639602768	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.6160014958552344	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	501	0.520432966987306	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	271	0.28151164481748486	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	221	0.22957222695448026	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	219	0.2274946502399601	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	205	0.21295161323831882	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	201	0.20879645980927844	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	199	0.20671888309475828	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	197	0.20464130638023809	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.20256372966571792	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	186	0.1932146344503771	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	182	0.1890594810213367	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	181	0.18802069266407662	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.17451644401969543	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	167	0.17347765566243534	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	165	0.17140007894791515	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	159	0.1651673488043546	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	145	0.15062431180271332	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	140	0.14543037001641285	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	134	0.1391976398728523	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	126	0.13088733301477157	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124	0.1288097563002514	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.12465460287121102	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.12153823779943074	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	117	0.12153823779943074	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	110	0.1142667192986101	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	104	0.10803398915504955	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	99	0.1028400473687491	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	99	0.1028400473687491	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0020775767145201837	0.0
16	0.0	0.0	0.0	0.0031163650717802756	0.0
17	0.0	0.0	0.0	0.012465460287121102	0.0
18	0.0	0.0	0.0	0.015581825358901378	0.0
19	0.0	0.0	0.0	0.01662061371616147	0.0
20	0.0	0.0	0.0	0.01765940207342156	0.0
21	0.0	0.0	0.0	0.020775767145201837	0.0
22	0.0	0.0	0.0	0.02700849728876239	0.0
23	0.0	0.0	0.0	0.04570668771944404	0.0
24	0.0	0.0	0.0	0.07479276172272661	0.0
25	0.0	0.0	0.0	0.09452974051066836	0.0
26	0.0	0.0	0.0	0.12257702615669083	0.0
27	0.0	0.0	0.0	0.20775767145201837	0.0
28	0.0	0.0	0.0	0.49757962312758397	0.0
29	0.0	0.0	0.0	0.9369870982486028	0.0
30	0.0	0.0	0.0	1.5644152660336983	0.0
31	0.0	0.0	0.0	2.7527891467392434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTAGA	35	8.8102417E-4	26.42857	1
ATGCTGT	50	2.6772596E-4	22.2	37
ATACACA	60	3.6808357E-5	21.583334	37
GGGCAGT	440	0.0	21.443182	7
GCAGTCG	445	0.0	21.202248	9
AGTCGGT	430	0.0	21.081394	11
CAGTCGG	450	0.0	20.966667	10
GGCAGTC	455	0.0	20.736263	8
TGGGCAG	455	0.0	20.736263	6
TGACTGT	55	5.0961954E-4	20.181818	33
CTCTATG	665	0.0	19.195488	1
GGTGATT	460	0.0	18.902174	15
GTCGGTG	475	0.0	18.694736	12
ATTCCTC	480	0.0	18.5	19
GATTCCT	470	0.0	18.5	18
TCGGTGA	470	0.0	18.5	13
GTGATTC	475	0.0	18.305264	16
TCTATGG	710	0.0	18.239437	2
CGGTGAT	490	0.0	18.122448	14
TGATTCC	480	0.0	18.114584	17
>>END_MODULE