FastQCFastQC Report
Fri 10 Feb 2017
ERR1631352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987816
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137011.3869991982312495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112121.1350291957206606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100081.0131441483029227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47750.4833896191193502No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA20050.2029730233160832No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17210.1742227297391417No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15720.15913894895405623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.1521538424159965No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.1451687358779368No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.14061323161398478No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA12660.12816151995918268No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.12654178510977754No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA12370.12522575054463586No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12200.12350478226714287No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11900.12046777942450822No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG10840.10973703604719906No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10660.10791483434161826No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10070.10194206208443678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA405.9392976E-527.752
TAGCGAA358.868507E-426.42857210
GTTATAC705.100723E-621.1428593
AGTCGGT11800.020.22457711
CTAGACA555.1423896E-420.1818184
GCAGTCG11800.020.0677979
CAGTCGG12100.019.87603410
GGCAGTC12450.019.020088
TCGGTGA12650.018.86561413
GGGCAGT12950.018.5714267
AGTTCGC500.007034199318.58
TACACAG701.21897676E-418.55
GTCGGTG13000.018.512
CGGTGAT13000.017.93076914
AGTACAC752.0668954E-417.2666665
CGCTTAT4200.017.17857226
GGTATCA63100.017.1220281
GCGATGA650.001579721717.07692322
GTGATTC13650.016.94139116
TGGGCAG15250.016.8622956
Produced by FastQC (version 0.11.5)