FastQCFastQC Report
Fri 10 Feb 2017
ERR1631351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234336
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.3597398607128226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6860.2927420456097228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6010.2564693431653694No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4360.1860576266557422No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3560.15191861259046838No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3450.14722449815649324No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3450.14722449815649324No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT3110.13271541717875188No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT2980.1271678273931449No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2860.12204697528335381No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC2620.1118052710637717No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG2500.1066844189539806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.10455073057490098No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG2420.10327051754745323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAT200.001838947337.031
CTTCGAT250.005487527729.59999827
ATGTCGG250.005487527729.59999832
TCCTTCG250.005487527729.59999825
TATCGCC250.005487527729.59999833
GGTAGAG250.005487527729.5999989
CGGAAGC701.9093568E-723.78571320
ATACACA551.8942896E-523.54545437
ATGGGTA400.001926626723.1255
GGTATCA4250.023.070591
CCGGAAG753.714522E-722.219
GGTGTAT450.003816859820.5555551
TCGATGG555.126315E-420.18181826
AGACTTT759.214245E-619.7333346
GCGGGTC759.214245E-619.73333433
CTGCCCG851.2366709E-619.58823626
CTTATAC3450.019.30434637
TCGTTTA801.6077376E-518.530
TGCCCGG902.1373744E-618.527
CGAACGA609.206931E-418.516
Produced by FastQC (version 0.11.5)