##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234336
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05023982657381	34.0	33.0	34.0	31.0	34.0
2	33.1842567936638	34.0	33.0	34.0	31.0	34.0
3	33.264479209340436	34.0	34.0	34.0	31.0	34.0
4	36.493189266693975	37.0	37.0	37.0	35.0	37.0
5	36.48803000819336	37.0	37.0	37.0	35.0	37.0
6	36.536037996722655	37.0	37.0	37.0	35.0	37.0
7	36.54227263416632	37.0	37.0	37.0	35.0	37.0
8	36.54010480677318	37.0	37.0	37.0	35.0	37.0
9	38.38589888024034	39.0	39.0	39.0	37.0	39.0
10	38.35150809094633	39.0	39.0	39.0	37.0	39.0
11	38.40103953297829	39.0	39.0	39.0	37.0	39.0
12	38.369900484774	39.0	39.0	39.0	37.0	39.0
13	38.38826727434112	39.0	39.0	39.0	37.0	39.0
14	39.92677608220674	41.0	40.0	41.0	38.0	41.0
15	39.92361395602895	41.0	40.0	41.0	38.0	41.0
16	39.87806397651236	41.0	40.0	41.0	38.0	41.0
17	39.884093779871634	41.0	40.0	41.0	38.0	41.0
18	39.85288218626246	41.0	40.0	41.0	38.0	41.0
19	39.86486071282261	41.0	40.0	41.0	38.0	41.0
20	39.83852246347126	41.0	40.0	41.0	38.0	41.0
21	39.80404632664209	41.0	40.0	41.0	38.0	41.0
22	39.743991533524515	41.0	40.0	41.0	38.0	41.0
23	39.661033729345895	41.0	40.0	41.0	37.0	41.0
24	39.63155469070053	41.0	40.0	41.0	37.0	41.0
25	39.6262034002458	41.0	40.0	41.0	37.0	41.0
26	39.5284762051072	41.0	40.0	41.0	37.0	41.0
27	39.470819677727704	41.0	39.0	41.0	36.0	41.0
28	39.371321521234464	41.0	39.0	41.0	36.0	41.0
29	39.31115577632118	41.0	39.0	41.0	36.0	41.0
30	39.242677181482996	41.0	39.0	41.0	35.0	41.0
31	39.18378311484364	41.0	39.0	41.0	35.0	41.0
32	39.107409873002865	41.0	39.0	41.0	35.0	41.0
33	39.02030844599208	41.0	39.0	41.0	35.0	41.0
34	38.95011436569712	41.0	39.0	41.0	35.0	41.0
35	38.83303461696026	40.0	38.0	41.0	35.0	41.0
36	38.76917759115117	40.0	38.0	41.0	35.0	41.0
37	38.68950139969958	40.0	38.0	41.0	35.0	41.0
38	38.60105148163321	40.0	38.0	41.0	35.0	41.0
39	38.497759627201965	40.0	38.0	41.0	35.0	41.0
40	38.38907807592517	40.0	38.0	41.0	35.0	41.0
41	38.280264235968865	40.0	37.0	41.0	35.0	41.0
42	38.12959596476854	40.0	37.0	41.0	34.0	41.0
43	37.3164473235013	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	2.0
18	6.0
19	13.0
20	26.0
21	44.0
22	54.0
23	83.0
24	158.0
25	243.0
26	351.0
27	510.0
28	733.0
29	1010.0
30	1310.0
31	1689.0
32	2124.0
33	2828.0
34	4277.0
35	6398.0
36	10585.0
37	20628.0
38	44751.0
39	136510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.178171514406664	17.935784514543222	12.66216031681005	26.223883654240066
2	19.708452819882563	19.90091151167554	33.14343506759525	27.24720060084665
3	20.45695070326369	21.34755223269152	29.05102075652055	29.144476307524236
4	14.880342755701214	15.513194728936227	32.823808548409126	36.78265396695343
5	16.535231462515362	34.682677864263276	31.79323706131367	16.98885361190769
6	34.74370135190495	33.261214666120445	15.86482657380855	16.130257408166052
7	30.256127953024713	29.69326095862351	20.033626928854297	20.016984159497472
8	28.646900177522873	30.49168715007511	19.865919022258637	20.995493650143384
9	27.391011197596615	14.24663730711457	19.20148846101325	39.16086303427557
10	19.50788611224908	24.79303222722928	29.546463198142835	26.152618462378808
11	37.68819131503482	20.931909736446812	19.887255906049432	21.492643042468934
12	23.553359278984022	24.926174382083847	26.371961627748192	25.14850471118394
13	30.82411579953571	17.76508944421685	24.655195957940734	26.755598798306707
14	24.28308070462925	20.42281168919842	22.830038918476035	32.464068687696304
15	27.52543356547863	25.993445309299467	20.671173016523284	25.80994810869862
16	27.024016796394918	24.465724429878467	22.66318448723201	25.847074286494603
17	25.745937457326235	24.983783968318996	23.25549638126451	26.014782193090262
18	25.713078656288406	22.476273385224633	25.496722654649734	26.313925303837227
19	25.91065820019118	24.35733306022122	24.506264509080978	25.225744230506624
20	26.32630069643589	22.572716099959035	24.676106104055716	26.424877099549366
21	28.218028813327873	23.591338932131638	23.755206199644956	24.435426054895533
22	27.60352656015294	22.696043288269834	23.567441622285948	26.132988529291275
23	25.60426054895535	23.019083708862485	24.565581046019393	26.811074696162773
24	25.55390550320907	24.079526833265056	24.387631435204153	25.978936228321725
25	25.847501024170423	22.993479448313533	24.269425099003143	26.889594428512904
26	26.139389594428515	24.268571623651507	24.4827939369111	25.109244845008877
27	26.951898129182027	22.687081797077703	24.018930083299196	26.342089990441075
28	24.863443943738904	23.731308889799262	25.292315307933904	26.112931858527926
29	24.640260139287175	24.0270380991397	25.527021029632664	25.805680731940463
30	24.07781988256179	25.107537894305615	25.44508739587601	25.36955482725659
31	25.83000477946197	24.076539669534345	23.9468114160863	26.146644134917384
32	24.556619554827254	23.529035231462515	24.55832650553052	27.356018708179707
33	24.133722518093677	23.06303768947153	25.53256861941827	27.270671173016524
34	25.63711934999317	23.05877031271337	25.20227365833675	26.101836678956715
35	25.58633756657108	23.016096545131777	25.85304861395603	25.544517274341118
36	23.876399699576677	24.132442305066228	25.815495698484227	26.175662296872865
37	25.32218694524102	22.208282124812236	26.20980131093814	26.259729619008603
38	23.80556124539123	21.976136829168375	27.510071009149257	26.708230916291136
39	24.391472074286494	21.566468660385087	27.554024989758297	26.488034275570122
40	23.525621330055987	22.145124948791477	28.216321862624604	26.112931858527926
41	22.33459647685375	22.433599617643043	29.36296599754199	25.86883790796122
42	23.582804178615323	22.22663184487232	28.872644408029497	25.31791956848286
43	21.61426328007647	21.56518844735764	29.188430288133276	27.632117984432607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	29.5
2	29.0
3	77.5
4	126.0
5	126.0
6	154.0
7	182.0
8	186.5
9	191.0
10	271.5
11	352.0
12	352.0
13	611.0
14	870.0
15	1309.5
16	1749.0
17	1613.0
18	1477.0
19	1477.0
20	1672.0
21	1867.0
22	1622.5
23	1378.0
24	1520.0
25	1662.0
26	1662.0
27	1831.0
28	2000.0
29	2520.5
30	3041.0
31	3458.0
32	3875.0
33	3875.0
34	4708.5
35	5542.0
36	6586.5
37	7631.0
38	8813.0
39	9995.0
40	9995.0
41	10916.0
42	11837.0
43	12058.0
44	12279.0
45	13512.0
46	14745.0
47	14745.0
48	15754.5
49	16764.0
50	17467.0
51	18170.0
52	18876.5
53	19583.0
54	19583.0
55	18232.0
56	16881.0
57	16388.0
58	15895.0
59	15109.0
60	14323.0
61	14323.0
62	13605.5
63	12888.0
64	11912.0
65	10936.0
66	9905.5
67	8875.0
68	8875.0
69	7638.0
70	6401.0
71	5528.5
72	4656.0
73	3639.0
74	2622.0
75	2622.0
76	2101.5
77	1581.0
78	1378.5
79	1176.0
80	1061.0
81	946.0
82	946.0
83	783.0
84	620.0
85	562.0
86	504.0
87	476.0
88	448.0
89	448.0
90	304.5
91	161.0
92	99.0
93	37.0
94	22.0
95	7.0
96	7.0
97	4.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	234336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.45582835188603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97829108182788	42.52127605731373
2	6.275914003927915	6.207610514589136
3	2.0188706263217715	2.995347574801025
4	1.0729766719873957	2.122598004615462
5	0.6955557640227118	1.7199643237336093
6	0.5077512089591184	1.5066753981446863
7	0.4100854863400987	1.419678219542494
8	0.29958257039678626	1.185288334300822
9	0.29460129404886676	1.3112775927651832
>10	2.095041384289139	20.83757845695514
>50	0.23488345115944964	8.09010259967366
>100	0.11346065013634432	9.034177604916668
>500	0.0029858065825353773	1.0484253186483874
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.3597398607128226	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.2927420456097228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.2564693431653694	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	436	0.1860576266557422	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	356	0.15191861259046838	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	345	0.14722449815649324	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	345	0.14722449815649324	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	311	0.13271541717875188	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	298	0.1271678273931449	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	286	0.12204697528335381	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	262	0.1118052710637717	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	250	0.1066844189539806	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.10455073057490098	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	242	0.10327051754745323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.2673767581592243E-4	0.0	0.0	0.0
6	0.0	4.2673767581592243E-4	0.0	0.0	0.0
7	0.0	4.2673767581592243E-4	0.0	4.2673767581592243E-4	0.0
8	0.0	4.2673767581592243E-4	0.0	4.2673767581592243E-4	0.0
9	0.0	4.2673767581592243E-4	0.0	4.2673767581592243E-4	0.0
10	0.0	4.2673767581592243E-4	0.0	4.2673767581592243E-4	0.0
11	0.0	8.534753516318449E-4	0.0	8.534753516318449E-4	0.0
12	0.0	8.534753516318449E-4	0.0	8.534753516318449E-4	0.0
13	0.0	8.534753516318449E-4	0.0	0.0012802130274477672	0.0
14	0.0	8.534753516318449E-4	0.0	0.002987163730711457	0.0
15	0.0	8.534753516318449E-4	0.0	0.0042673767581592244	0.0
16	0.0	8.534753516318449E-4	0.0	0.006401065137238837	0.0
17	0.0	8.534753516318449E-4	0.0	0.006401065137238837	0.0
18	0.0	8.534753516318449E-4	0.0	0.006827802813054759	0.0
19	0.0	8.534753516318449E-4	0.0	0.008108015840502527	0.0
20	0.0	8.534753516318449E-4	0.0	0.008961491192134371	0.0
21	0.0	8.534753516318449E-4	0.0	0.010241704219582138	0.0
22	0.0	8.534753516318449E-4	0.0	0.013228867950293596	0.0
23	0.0	8.534753516318449E-4	0.0	0.018349720060084666	0.0
24	0.0	8.534753516318449E-4	0.0	0.025177522873139423	0.0
25	0.0	8.534753516318449E-4	0.0	0.029018161955482725	0.0
26	0.0	8.534753516318449E-4	0.0	0.03925986617506486	0.0
27	0.0	8.534753516318449E-4	0.0	0.0819336337566571	0.0
28	0.0	8.534753516318449E-4	0.0	0.2547623924621057	0.0
29	0.0	8.534753516318449E-4	0.0	0.4527686740406937	0.0
30	0.0	8.534753516318449E-4	0.0	0.7284412126177796	0.0
31	0.0	8.534753516318449E-4	0.0	1.414208657653967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAT	20	0.0018389473	37.0	31
CTTCGAT	25	0.0054875277	29.599998	27
ATGTCGG	25	0.0054875277	29.599998	32
TCCTTCG	25	0.0054875277	29.599998	25
TATCGCC	25	0.0054875277	29.599998	33
GGTAGAG	25	0.0054875277	29.599998	9
CGGAAGC	70	1.9093568E-7	23.785713	20
ATACACA	55	1.8942896E-5	23.545454	37
ATGGGTA	40	0.0019266267	23.125	5
GGTATCA	425	0.0	23.07059	1
CCGGAAG	75	3.714522E-7	22.2	19
GGTGTAT	45	0.0038168598	20.555555	1
TCGATGG	55	5.126315E-4	20.181818	26
AGACTTT	75	9.214245E-6	19.733334	6
GCGGGTC	75	9.214245E-6	19.733334	33
CTGCCCG	85	1.2366709E-6	19.588236	26
CTTATAC	345	0.0	19.304346	37
TCGTTTA	80	1.6077376E-5	18.5	30
TGCCCGG	90	2.1373744E-6	18.5	27
CGAACGA	60	9.206931E-4	18.5	16
>>END_MODULE