FastQCFastQC Report
Fri 10 Feb 2017
ERR1631350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744323
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38660.5193981645065382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31680.4256216723116174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29290.39351195650275483No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12750.1712966010723839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.15571196913168076No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11010.14791965316132916No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC9610.12911061461220466No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9160.12306485222141463No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8870.11916869423623884No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8730.11728779038132639No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8150.10949547441097479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAG400.001930551423.1255
GCAGTCG5350.022.1308429
GGCAGTC5500.021.5272718
TATACAC2850.021.42105337
TAACGGC705.097936E-621.14285736
CAGTCGG5550.021.010
CCGAGTT450.00382456120.55555513
TGTACGG450.00382456120.55555510
TTAACGG656.897953E-519.92307735
AAGACGG1600.019.656255
GGTATCA17000.019.5882361
AGTCGGT6050.019.26446211
GTGTAAG1457.2759576E-1219.1379321
TAATACT1650.019.0606064
GCGACTA1650.019.06060615
TTCCTCG4200.018.94047520
AAGCTAG801.6154234E-518.55
CCAAACG500.007032611418.4999989
TCGGTGA6350.018.06299213
AGGCACG1750.017.971438
Produced by FastQC (version 0.11.5)