##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744323
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94769072029213	34.0	33.0	34.0	31.0	34.0
2	33.0854427446149	34.0	33.0	34.0	31.0	34.0
3	33.16100536998051	34.0	33.0	34.0	31.0	34.0
4	36.43094463022102	37.0	37.0	37.0	35.0	37.0
5	36.41425563901693	37.0	37.0	37.0	35.0	37.0
6	36.47632412272629	37.0	37.0	37.0	35.0	37.0
7	36.49001978979556	37.0	37.0	37.0	35.0	37.0
8	36.48873943167146	37.0	37.0	37.0	35.0	37.0
9	38.32090772420038	39.0	39.0	39.0	37.0	39.0
10	38.26219396686653	39.0	39.0	39.0	37.0	39.0
11	38.322455439372426	39.0	39.0	39.0	37.0	39.0
12	38.28117631727086	39.0	39.0	39.0	37.0	39.0
13	38.317647043017615	39.0	39.0	39.0	37.0	39.0
14	39.813398215559644	41.0	40.0	41.0	38.0	41.0
15	39.78884838974477	41.0	40.0	41.0	38.0	41.0
16	39.754136309102364	41.0	40.0	41.0	38.0	41.0
17	39.74839552183662	41.0	40.0	41.0	38.0	41.0
18	39.73765018681406	41.0	40.0	41.0	38.0	41.0
19	39.76227659228588	41.0	40.0	41.0	38.0	41.0
20	39.72804279862372	41.0	40.0	41.0	38.0	41.0
21	39.71313126156252	41.0	40.0	41.0	37.0	41.0
22	39.653419281682815	41.0	40.0	41.0	37.0	41.0
23	39.59664554232504	41.0	40.0	41.0	37.0	41.0
24	39.571401125586604	41.0	40.0	41.0	37.0	41.0
25	39.55747840655199	41.0	40.0	41.0	37.0	41.0
26	39.45619173396496	41.0	39.0	41.0	37.0	41.0
27	39.4095144177998	41.0	39.0	41.0	36.0	41.0
28	39.32988366609657	41.0	39.0	41.0	36.0	41.0
29	39.27486991534589	41.0	39.0	41.0	36.0	41.0
30	39.189282072433606	41.0	39.0	41.0	35.0	41.0
31	39.12477244422112	41.0	39.0	41.0	35.0	41.0
32	39.03958227812388	40.0	39.0	41.0	35.0	41.0
33	38.952868579904155	40.0	39.0	41.0	35.0	41.0
34	38.89889201327918	40.0	39.0	41.0	35.0	41.0
35	38.78634410061223	40.0	38.0	41.0	35.0	41.0
36	38.72120033910009	40.0	38.0	41.0	35.0	41.0
37	38.644969992866	40.0	38.0	41.0	35.0	41.0
38	38.55207349497463	40.0	38.0	41.0	35.0	41.0
39	38.44112435058436	40.0	38.0	41.0	35.0	41.0
40	38.32105819650877	40.0	38.0	41.0	35.0	41.0
41	38.206016742731315	40.0	38.0	41.0	35.0	41.0
42	38.05535634395283	40.0	37.0	41.0	34.0	41.0
43	37.24185870918942	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	2.0
14	4.0
15	8.0
16	9.0
17	4.0
18	13.0
19	31.0
20	60.0
21	96.0
22	163.0
23	291.0
24	505.0
25	738.0
26	1097.0
27	1674.0
28	2329.0
29	3463.0
30	4539.0
31	5972.0
32	7403.0
33	9882.0
34	14569.0
35	21222.0
36	33196.0
37	68816.0
38	160082.0
39	408145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73141767754053	17.72348832428932	12.404963973973665	26.140130024196484
2	19.150288248515764	19.627231726011424	34.16016971126783	27.06231031420499
3	19.398836257914912	22.82167822302952	29.052709643528413	28.726775875527157
4	13.472914312737885	15.914193166138894	34.84172865812288	35.77116386300034
5	14.68824690356203	36.915828208989915	33.13131530263072	15.264609584817343
6	33.221195636840456	35.10854830496975	16.673809622972822	14.99644643521697
7	30.454386066264245	30.031317049184292	20.696928618355205	18.81736826619626
8	29.66722780298338	31.350905453680728	18.90079978718916	20.081066956146724
9	28.067250373829637	13.82706163856283	19.11320757251892	38.99248041508861
10	18.96515356908224	25.34343289136571	30.572748658848376	25.118664880703673
11	36.848653071314466	22.21320582596534	19.987021763401103	20.951119339319085
12	23.511432536680985	25.999330935628752	26.93991721336033	23.549319314329935
13	30.859855197273227	18.41848229867947	24.84029111017663	25.881371393870673
14	23.22244509440122	19.96216696246119	24.658246487076173	32.15714145606142
15	26.912509757188747	26.311695325819574	21.73720280039714	25.038592116594543
16	25.836901452729528	25.040204319898752	23.52742021944774	25.59547400792398
17	24.31874334126448	26.208245613799384	24.269168089660003	25.203842955276134
18	24.565410446808713	23.74345546221197	26.365569786235277	25.32556430474404
19	25.279482160298688	24.899539581606373	25.75750043999715	24.063477818097788
20	25.26000137037281	23.61044868961459	26.094450930577185	25.03509900943542
21	27.053577546307185	23.9821959015105	25.242804535127895	23.721422017054426
22	26.903105237914186	23.522046208433704	24.6992233210582	24.875625232593915
23	25.01091595987226	23.798404724830483	25.463004636428003	25.727674678869256
24	24.38968028664975	24.728780381635392	25.276392103965616	25.605147227749242
25	24.79205936132566	23.755815687544253	25.732108237955835	25.720016713174253
26	25.001377090322347	24.89013506233181	25.70147637517583	24.407011472170012
27	25.675143721207057	24.037010813853662	25.02327618520454	25.26456927973474
28	24.098946290790423	24.410370229053786	26.491187293688355	24.999496186467436
29	24.26339102781991	24.347091249363515	26.729255981610134	24.660261741206437
30	23.538436942026514	25.22359244575272	26.71192479608987	24.5260458161309
31	24.77687778021101	24.87038557185523	25.4244461074023	24.928290540531464
32	23.99455612684278	24.366706389564747	26.191720529931228	25.447016953661244
33	23.592848803543625	24.07933115058919	26.74497496382619	25.582845082040993
34	25.14701278880271	23.80995884851066	26.11675307628543	24.9262752864012
35	24.65878388817758	24.25909181900868	26.552451019248362	24.529673273565376
36	23.732304389357846	24.957041499456555	25.808419194355135	25.502234916830464
37	24.237998825778593	23.43377807752817	26.724016320871453	25.604206775821787
38	23.499341011899403	22.888853360704964	27.701280223773818	25.910525403621815
39	23.57135275948748	23.118592331554982	27.783905643114615	25.526149265842918
40	23.261003623426927	22.858758899026363	28.384720074483795	25.495517403062916
41	21.83259149589627	23.14828374240753	29.36749233867555	25.651632423020647
42	22.402639714210093	23.269467690774032	29.069772128497977	25.258120466517898
43	21.458560329319397	22.480965924739664	29.451461260769857	26.609012485171093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	103.0
2	96.0
3	292.0
4	488.0
5	488.0
6	636.0
7	784.0
8	728.5
9	673.0
10	1076.5
11	1480.0
12	1480.0
13	2491.5
14	3503.0
15	5469.5
16	7436.0
17	6707.0
18	5978.0
19	5978.0
20	6431.5
21	6885.0
22	5358.5
23	3832.0
24	4051.0
25	4270.0
26	4270.0
27	4967.0
28	5664.0
29	7412.0
30	9160.0
31	11383.0
32	13606.0
33	13606.0
34	17397.5
35	21189.0
36	25504.0
37	29819.0
38	33207.0
39	36595.0
40	36595.0
41	40148.0
42	43701.0
43	46878.0
44	50055.0
45	51846.0
46	53637.0
47	53637.0
48	57216.5
49	60796.0
50	60105.5
51	59415.0
52	57924.0
53	56433.0
54	56433.0
55	53040.0
56	49647.0
57	45969.5
58	42292.0
59	40548.0
60	38804.0
61	38804.0
62	36858.0
63	34912.0
64	32178.0
65	29444.0
66	26438.0
67	23432.0
68	23432.0
69	19848.0
70	16264.0
71	14185.0
72	12106.0
73	9976.0
74	7846.0
75	7846.0
76	6817.0
77	5788.0
78	4655.5
79	3523.0
80	2716.0
81	1909.0
82	1909.0
83	1486.5
84	1064.0
85	905.0
86	746.0
87	662.5
88	579.0
89	579.0
90	437.0
91	295.0
92	170.5
93	46.0
94	30.0
95	14.0
96	14.0
97	10.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	744323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.1208028436143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5673731867271	42.522588709948046
2	6.381588790445569	6.269375296089915
3	2.051401853219266	3.0229951795502568
4	1.0416812228304657	2.0467287189020142
5	0.6509884418012962	1.5988537451596576
6	0.46201833507322554	1.3616826928360104
7	0.3410246944141826	1.1725984745386013
8	0.2661978813883358	1.0460682919251414
9	0.20978415126827787	0.9274289340747633
>10	1.6622926406546847	16.817474730682456
>50	0.22256886257384947	7.6282932316392325
>100	0.13650155157607116	12.198776645228957
>500	0.0049337910208198926	1.5697663091364766
>1k	0.001644597006939964	1.8173690402885463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3866	0.5193981645065382	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3168	0.4256216723116174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2929	0.39351195650275483	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1275	0.1712966010723839	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1159	0.15571196913168076	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1101	0.14791965316132916	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	961	0.12911061461220466	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	916	0.12306485222141463	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	887	0.11916869423623884	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	873	0.11728779038132639	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	815	0.10949547441097479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	1.3435027535088932E-4
4	0.0	0.0	0.0	1.3435027535088932E-4	1.3435027535088932E-4
5	0.0	0.0	0.0	1.3435027535088932E-4	2.6870055070177864E-4
6	0.0	0.0	0.0	1.3435027535088932E-4	2.6870055070177864E-4
7	0.0	0.0	0.0	1.3435027535088932E-4	2.6870055070177864E-4
8	0.0	0.0	0.0	1.3435027535088932E-4	2.6870055070177864E-4
9	0.0	0.0	0.0	1.3435027535088932E-4	2.6870055070177864E-4
10	0.0	0.0	0.0	2.6870055070177864E-4	2.6870055070177864E-4
11	0.0	0.0	0.0	9.404519274562253E-4	2.6870055070177864E-4
12	0.0	0.0	0.0	0.0010748022028071146	2.6870055070177864E-4
13	0.0	0.0	0.0	0.0014778530288597827	2.6870055070177864E-4
14	0.0	0.0	0.0	0.001612203304210672	2.6870055070177864E-4
15	0.0	0.0	0.0	0.0017465535795615614	2.6870055070177864E-4
16	0.0	0.0	0.0	0.002418304956316008	2.6870055070177864E-4
17	0.0	0.0	0.0	0.003627457434474012	2.6870055070177864E-4
18	0.0	0.0	0.0	0.004299208811228458	2.6870055070177864E-4
19	0.0	0.0	0.0	0.004836609912632016	2.6870055070177864E-4
20	0.0	0.0	0.0	0.005642711564737352	2.6870055070177864E-4
21	0.0	0.0	0.0	0.007926666245702471	4.03050826052668E-4
22	0.0	0.0	0.0	0.010748022028071147	4.03050826052668E-4
23	0.0	0.0	0.0	0.015047230839299605	4.03050826052668E-4
24	0.0	0.0	0.0	0.020824292679387846	4.03050826052668E-4
25	0.0	0.0	0.0	0.024854800939914528	4.03050826052668E-4
26	0.0	0.0	0.0	0.032781467185616996	4.03050826052668E-4
27	0.0	0.0	0.0	0.06596598519728666	4.03050826052668E-4
28	0.0	0.0	0.0	0.18526902970887638	4.03050826052668E-4
29	0.0	0.0	0.0	0.3585808849115236	4.03050826052668E-4
30	0.0	0.0	0.0	0.6048449396297038	4.03050826052668E-4
31	0.0	0.0	0.0	1.1755649093202816	4.03050826052668E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATAG	40	0.0019305514	23.125	5
GCAGTCG	535	0.0	22.130842	9
GGCAGTC	550	0.0	21.527271	8
TATACAC	285	0.0	21.421053	37
TAACGGC	70	5.097936E-6	21.142857	36
CAGTCGG	555	0.0	21.0	10
CCGAGTT	45	0.003824561	20.555555	13
TGTACGG	45	0.003824561	20.555555	10
TTAACGG	65	6.897953E-5	19.923077	35
AAGACGG	160	0.0	19.65625	5
GGTATCA	1700	0.0	19.588236	1
AGTCGGT	605	0.0	19.264462	11
GTGTAAG	145	7.2759576E-12	19.137932	1
TAATACT	165	0.0	19.060606	4
GCGACTA	165	0.0	19.060606	15
TTCCTCG	420	0.0	18.940475	20
AAGCTAG	80	1.6154234E-5	18.5	5
CCAAACG	50	0.0070326114	18.499998	9
TCGGTGA	635	0.0	18.062992	13
AGGCACG	175	0.0	17.97143	8
>>END_MODULE