FastQCFastQC Report
Fri 10 Feb 2017
ERR1631349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118197
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.42783158960362083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46250.41361227046754734No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC39090.34958061951516595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36220.32391430132615273No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT30060.26882561838388047No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT18450.1649977597865135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.159631978980448No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG17590.15730680729781962No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT16860.15077844065043994No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT14650.13101448134809876No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14110.12618527862263984No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG13920.1244861147007191No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG13760.12305523981910164No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG13510.12081949781657436No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT12690.11348626404828488No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG12640.11303911564777942No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT12300.1099985065243423No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT12230.10937249876363467No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG11910.10651074900039975No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG11260.10069781979382882No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC11200.10016124171322227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA603.7261696E-521.5833341
GGACCGT555.142973E-420.1818186
GGTATCA24700.019.773281
CAATAGT759.265401E-619.7333344
CGAACTA500.00703476718.49999824
TGCATCA10250.017.86829214
AGTCGGT4100.017.5975611
CAGGACA10850.017.2211974
GCATCAG10850.016.88018215
CTGCATC10750.016.86511613
CTAGATA2750.016.8181823
GCAGTCG4300.016.779079
TACACTT904.4457833E-516.4444455
GACAGGC11650.016.3562227
CGCTTAT1251.6574813E-716.27999926
GGGCAGT4550.016.2637357
GCTGCAT11200.016.187512
ATACACA5950.016.16806637
TATATTG1059.342561E-615.8571445
TCTAGAT3150.015.8571422
Produced by FastQC (version 0.11.5)