##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118197
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69761678845499	34.0	31.0	34.0	31.0	34.0
2	32.86131066350563	34.0	31.0	34.0	31.0	34.0
3	32.92778374472476	34.0	31.0	34.0	31.0	34.0
4	36.33010641237635	37.0	37.0	37.0	35.0	37.0
5	36.2164708007623	37.0	37.0	37.0	35.0	37.0
6	36.25188584837913	37.0	37.0	37.0	35.0	37.0
7	36.260788573033196	37.0	37.0	37.0	35.0	37.0
8	36.246012106990094	37.0	37.0	37.0	35.0	37.0
9	38.00891435051248	39.0	38.0	39.0	35.0	39.0
10	37.93590664256835	39.0	38.0	39.0	35.0	39.0
11	38.00252012838525	39.0	38.0	39.0	35.0	39.0
12	37.95016441646687	39.0	38.0	39.0	35.0	39.0
13	37.982100649527766	39.0	38.0	39.0	35.0	39.0
14	39.38580947722092	40.0	39.0	41.0	37.0	41.0
15	39.353262439444926	40.0	39.0	41.0	37.0	41.0
16	39.3038748986091	40.0	39.0	41.0	36.0	41.0
17	39.2926389536012	40.0	39.0	41.0	36.0	41.0
18	39.21329515282191	40.0	39.0	41.0	36.0	41.0
19	39.2338103214371	40.0	39.0	41.0	36.0	41.0
20	39.21492456159335	40.0	39.0	41.0	36.0	41.0
21	39.18299190571965	40.0	39.0	41.0	36.0	41.0
22	39.11433584600925	40.0	39.0	41.0	36.0	41.0
23	39.0502469600616	40.0	39.0	41.0	35.0	41.0
24	38.99432568679759	40.0	39.0	41.0	35.0	41.0
25	38.97511350862147	40.0	38.0	41.0	35.0	41.0
26	38.829790278457196	40.0	38.0	41.0	35.0	41.0
27	38.780510053237485	40.0	38.0	41.0	35.0	41.0
28	38.68245040900664	40.0	38.0	41.0	35.0	41.0
29	38.59844195611328	40.0	38.0	41.0	35.0	41.0
30	38.50512119063099	40.0	38.0	41.0	35.0	41.0
31	38.454703419880396	40.0	38.0	41.0	35.0	41.0
32	38.35736815605837	40.0	38.0	41.0	34.0	41.0
33	38.24306271614036	40.0	38.0	41.0	34.0	41.0
34	38.15478578461577	40.0	38.0	41.0	34.0	41.0
35	38.0209649999061	40.0	37.0	41.0	34.0	41.0
36	37.93709158582969	40.0	37.0	41.0	33.0	41.0
37	37.847156628036025	40.0	37.0	41.0	33.0	41.0
38	37.75863465918796	40.0	37.0	41.0	33.0	41.0
39	37.64480230227768	40.0	37.0	41.0	33.0	41.0
40	37.49315639373027	40.0	36.0	41.0	33.0	41.0
41	37.352980735952606	40.0	36.0	41.0	33.0	41.0
42	37.201923274700256	40.0	36.0	41.0	33.0	41.0
43	36.285974653840064	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	3.0
14	6.0
15	9.0
16	12.0
17	25.0
18	48.0
19	72.0
20	146.0
21	261.0
22	447.0
23	689.0
24	1153.0
25	1809.0
26	2559.0
27	3623.0
28	5170.0
29	7048.0
30	9451.0
31	12100.0
32	15766.0
33	21923.0
34	33173.0
35	48957.0
36	77521.0
37	149334.0
38	315405.0
39	411477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76348711363025	17.17228717301155	12.358019204129505	26.706206509228696
2	20.46124251808939	18.992449452109064	33.28054001218032	27.265768017621223
3	21.799825969842523	20.94818712623983	27.784013013807048	29.4679738901106
4	16.20519461239835	15.119428866291	31.051058087260113	37.62431843405053
5	17.259033962709612	34.34636293962513	30.504195593441942	17.890407504223315
6	36.13307851836483	31.17098328827568	15.630430058388638	17.065508134970848
7	31.05472470414426	28.67249688561139	19.372615022218802	20.900163388025543
8	29.215335043825014	30.764972540616725	18.868678774849155	21.151013640709106
9	28.147634093098084	13.877518898727148	18.759753424486025	39.21509358368874
10	20.976268045791574	24.357693680093938	28.645220833180556	26.02081744093393
11	37.30854223361358	20.911789246438687	19.0281318944694	22.751536625478337
12	24.88819054245361	24.04549466686103	25.719707708033557	25.346607082651808
13	31.523067938833677	17.51533942587934	24.00471473273493	26.95687790255206
14	24.356709953612825	19.748130248963285	23.108897627162296	32.786262170261594
15	28.09549658959915	24.62768188431913	20.71531223925659	26.56150928682513
16	26.69762125993899	23.848123362877917	22.024473326256462	27.429782050926626
17	25.575636493390704	24.72918457123387	23.488705478551633	26.206473456823797
18	25.348395676253826	21.839264458767104	25.31459125717561	27.49774860780346
19	26.294203973002965	23.4696569566901	24.345978392000696	25.89016067830624
20	26.399015558081445	22.69188702885091	24.5352115950946	26.37388581797304
21	29.33937401012523	22.254575893156574	23.49264038447608	24.913409712242117
22	28.318265922730966	22.719252510961844	23.05497153006134	25.907510036245853
23	26.757270856566418	22.928517962398395	23.497201298161237	26.81700988287395
24	26.474315348726567	23.418771468712578	24.118111567103114	25.988801615457742
25	26.230619470451096	22.588327459293843	24.219077675937246	26.961975394317818
26	27.045144996811832	23.42208036687632	24.441399860668557	25.09137477564329
27	27.690201279381004	22.310827161940157	23.448104403785738	26.550867154893098
28	25.575457634030496	22.347940479182114	25.258339988392027	26.818261898395363
29	25.355639480342013	22.58117308488576	25.681610664310494	26.381576770461734
30	24.824695469581837	23.1664903411474	25.79849525620262	26.210318933068145
31	25.682057812710994	23.285700104722153	24.341596337675742	26.690645744891107
32	24.98388030016178	22.8302347439673	25.214698304502697	26.971186651368228
33	25.68554557023494	22.79124340344322	24.95454736508862	26.568663661233217
34	26.302968081652878	22.89283552003806	24.866637989549247	25.937558408759813
35	26.169181280221643	22.52223892569914	25.656302064841885	25.652277729237333
36	24.86949973931248	23.079475262409037	24.85393897497489	27.197086023303584
37	24.420741604565208	22.19206454676591	26.13761260314596	27.249581245522926
38	24.957230255491652	21.238923016248478	27.144501371404147	26.659345356855724
39	23.963129931487924	21.485391214607084	27.585747413022926	26.965731440882063
40	24.015714583387364	21.030283572572632	28.417980016043686	26.53602182799632
41	22.485215038137287	21.534398679302484	29.270244867407087	26.710141415153142
42	22.80751960522162	22.190723101564394	28.411988227476915	26.589769065737073
43	21.739908084174793	20.823611581859012	29.466095866828475	27.97038446713772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	768.0
1	543.0
2	318.0
3	653.5
4	989.0
5	989.0
6	1127.0
7	1265.0
8	1293.0
9	1321.0
10	1887.5
11	2454.0
12	2454.0
13	4194.0
14	5934.0
15	8196.0
16	10458.0
17	9667.5
18	8877.0
19	8877.0
20	10006.5
21	11136.0
22	9162.5
23	7189.0
24	7689.5
25	8190.0
26	8190.0
27	9272.5
28	10355.0
29	12107.0
30	13859.0
31	16037.0
32	18215.0
33	18215.0
34	21462.5
35	24710.0
36	28890.5
37	33071.0
38	36765.5
39	40460.0
40	40460.0
41	43383.5
42	46307.0
43	49081.5
44	51856.0
45	55065.5
46	58275.0
47	58275.0
48	62714.5
49	67154.0
50	72285.5
51	77417.0
52	82179.5
53	86942.0
54	86942.0
55	86790.0
56	86638.0
57	83543.5
58	80449.0
59	74698.0
60	68947.0
61	68947.0
62	67194.0
63	65441.0
64	59961.5
65	54482.0
66	48291.5
67	42101.0
68	42101.0
69	36787.0
70	31473.0
71	28949.5
72	26426.0
73	26490.5
74	26555.0
75	26555.0
76	25180.5
77	23806.0
78	18888.0
79	13970.0
80	9166.0
81	4362.0
82	4362.0
83	3341.5
84	2321.0
85	1996.5
86	1672.0
87	1472.5
88	1273.0
89	1273.0
90	923.5
91	574.0
92	350.0
93	126.0
94	83.5
95	41.0
96	41.0
97	26.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1118197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.43912498284732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.95422424555609	43.978190102282745
2	5.731790290323256	5.667493930775243
3	1.5839570758222898	2.3492835551713065
4	0.8405207004011502	1.6621843183121134
5	0.5095946693361023	1.2596957273950156
6	0.34396808071184765	1.0203288559453887
7	0.25364576314213627	0.8778017209747632
8	0.18756012975344147	0.741824695734356
9	0.1406158060705897	0.6256730169798935
>10	1.1306360096517305	11.158008514319546
>50	0.1499481541284046	5.270840476936706
>100	0.1530266113002521	16.075838624007442
>500	0.015248203180764716	4.751278673287315
>1k	0.005264260621930676	4.561557787878199
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4784	0.42783158960362083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4625	0.41361227046754734	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3909	0.34958061951516595	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3622	0.32391430132615273	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3006	0.26882561838388047	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1845	0.1649977597865135	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1785	0.159631978980448	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1759	0.15730680729781962	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1686	0.15077844065043994	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1465	0.13101448134809876	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1411	0.12618527862263984	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1392	0.1244861147007191	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1376	0.12305523981910164	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1351	0.12081949781657436	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1269	0.11348626404828488	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1264	0.11303911564777942	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1230	0.1099985065243423	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1223	0.10937249876363467	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1191	0.10651074900039975	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1126	0.10069781979382882	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1120	0.10016124171322227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.942968010109132E-5
2	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
3	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
4	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
5	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
6	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
7	8.942968010109132E-5	0.0	0.0	0.0	8.942968010109132E-5
8	8.942968010109132E-5	0.0	0.0	8.942968010109132E-5	8.942968010109132E-5
9	8.942968010109132E-5	0.0	0.0	4.4714840050545657E-4	8.942968010109132E-5
10	8.942968010109132E-5	0.0	0.0	6.260077607076392E-4	8.942968010109132E-5
11	8.942968010109132E-5	0.0	0.0	8.048671209098218E-4	8.942968010109132E-5
12	1.7885936020218263E-4	0.0	0.0	0.001162585841314187	8.942968010109132E-5
13	1.7885936020218263E-4	0.0	0.0	0.0015203045617185522	8.942968010109132E-5
14	1.7885936020218263E-4	0.0	0.0	0.0020568826423251	8.942968010109132E-5
15	1.7885936020218263E-4	0.0	0.0	0.0030406091234371044	8.942968010109132E-5
16	1.7885936020218263E-4	0.0	0.0	0.0038454762443469264	8.942968010109132E-5
17	2.682890403032739E-4	0.0	0.0	0.005365780806065479	8.942968010109132E-5
18	2.682890403032739E-4	0.0	0.0	0.006528366647379665	8.942968010109132E-5
19	2.682890403032739E-4	0.0	0.0	0.007333233768289487	8.942968010109132E-5
20	2.682890403032739E-4	0.0	0.0	0.007690952488693853	8.942968010109132E-5
21	2.682890403032739E-4	0.0	0.0	0.00956897577081677	1.7885936020218263E-4
22	2.682890403032739E-4	0.0	0.0	0.011715288093242961	1.7885936020218263E-4
23	2.682890403032739E-4	0.0	0.0	0.016902209539106258	1.7885936020218263E-4
24	2.682890403032739E-4	0.0	0.0	0.026202896269619753	1.7885936020218263E-4
25	2.682890403032739E-4	0.0	0.0	0.03344670035780815	1.7885936020218263E-4
26	2.682890403032739E-4	0.0	0.0	0.0556252610228788	1.7885936020218263E-4
27	2.682890403032739E-4	0.0	0.0	0.12716900510375184	1.7885936020218263E-4
28	2.682890403032739E-4	0.0	0.0	0.36210077472931873	1.7885936020218263E-4
29	2.682890403032739E-4	0.0	0.0	0.6472920245716989	1.7885936020218263E-4
30	2.682890403032739E-4	0.0	0.0	1.0346119690895255	1.7885936020218263E-4
31	2.682890403032739E-4	0.0	0.0	1.751122566059469	1.7885936020218263E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	60	3.7261696E-5	21.583334	1
GGACCGT	55	5.142973E-4	20.181818	6
GGTATCA	2470	0.0	19.77328	1
CAATAGT	75	9.265401E-6	19.733334	4
CGAACTA	50	0.007034767	18.499998	24
TGCATCA	1025	0.0	17.868292	14
AGTCGGT	410	0.0	17.59756	11
CAGGACA	1085	0.0	17.221197	4
GCATCAG	1085	0.0	16.880182	15
CTGCATC	1075	0.0	16.865116	13
CTAGATA	275	0.0	16.818182	3
GCAGTCG	430	0.0	16.77907	9
TACACTT	90	4.4457833E-5	16.444445	5
GACAGGC	1165	0.0	16.356222	7
CGCTTAT	125	1.6574813E-7	16.279999	26
GGGCAGT	455	0.0	16.263735	7
GCTGCAT	1120	0.0	16.1875	12
ATACACA	595	0.0	16.168066	37
TATATTG	105	9.342561E-6	15.857144	5
TCTAGAT	315	0.0	15.857142	2
>>END_MODULE