FastQCFastQC Report
Fri 10 Feb 2017
ERR1631348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454427
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75270.5175233958115464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52730.36254827502514736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50550.34755955438120995No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA22230.15284369720859142No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19630.13496724139472108No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.1267853250799112No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC16460.11317171642165609No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16060.11042149245029141No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC15980.1098714476560185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA250.00549681729.62
ATACACA10600.023.38679337
GCAGTCG18800.020.6648929
AGTCGGT18850.020.6100811
CGTGATA450.00382628920.5555552
GTTAGCC450.00382628920.5555559
CAGTCGG19100.020.43717410
GGCAGTC19650.019.8651398
GGGCAGT20600.019.128647
TCGGTGA21000.018.6761913
CGGTGAT21300.018.41314514
TATTGAC3750.018.25333230
GTCGGTG21600.018.24305512
GTGATTC21750.018.1172416
GGTATCA53500.017.912151
GGTGATT22100.017.83031715
TATTAGA1258.583811E-917.762
ATTGACT3750.017.75999831
TATACAC19400.017.64175237
GATTCCT21900.017.57077618
Produced by FastQC (version 0.11.5)