##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631348.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1454427
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04931426603054	34.0	33.0	34.0	31.0	34.0
2	33.17926234867752	34.0	33.0	34.0	31.0	34.0
3	33.23882738700533	34.0	33.0	34.0	31.0	34.0
4	36.50924590921373	37.0	37.0	37.0	35.0	37.0
5	36.50103924088318	37.0	37.0	37.0	35.0	37.0
6	36.55548955018024	37.0	37.0	37.0	35.0	37.0
7	36.55422719737739	37.0	37.0	37.0	35.0	37.0
8	36.55599352872299	37.0	37.0	37.0	35.0	37.0
9	38.40025659589653	39.0	39.0	39.0	37.0	39.0
10	38.36288792768561	39.0	39.0	39.0	37.0	39.0
11	38.42133568752505	39.0	39.0	39.0	37.0	39.0
12	38.3802054004773	39.0	39.0	39.0	37.0	39.0
13	38.40416741438381	39.0	39.0	39.0	37.0	39.0
14	39.91523534697857	41.0	40.0	41.0	38.0	41.0
15	39.90311029704482	41.0	40.0	41.0	38.0	41.0
16	39.86499150524571	41.0	40.0	41.0	38.0	41.0
17	39.859725513896535	41.0	40.0	41.0	38.0	41.0
18	39.87867524461523	41.0	40.0	41.0	38.0	41.0
19	39.89861574351961	41.0	40.0	41.0	38.0	41.0
20	39.89248343161946	41.0	40.0	41.0	38.0	41.0
21	39.86214708610333	41.0	40.0	41.0	38.0	41.0
22	39.802173639515765	41.0	40.0	41.0	38.0	41.0
23	39.734790401993365	41.0	40.0	41.0	37.0	41.0
24	39.69894879564254	41.0	40.0	41.0	37.0	41.0
25	39.67570321508058	41.0	40.0	41.0	37.0	41.0
26	39.59159999092426	41.0	40.0	41.0	37.0	41.0
27	39.52389497719721	41.0	40.0	41.0	37.0	41.0
28	39.426706187385136	41.0	39.0	41.0	36.0	41.0
29	39.34398082543847	41.0	39.0	41.0	36.0	41.0
30	39.25088918178774	41.0	39.0	41.0	35.0	41.0
31	39.17580256692155	41.0	39.0	41.0	35.0	41.0
32	39.09212356481281	41.0	39.0	41.0	35.0	41.0
33	39.00400707632628	41.0	39.0	41.0	35.0	41.0
34	38.933674223594586	41.0	39.0	41.0	35.0	41.0
35	38.835401845537795	40.0	39.0	41.0	35.0	41.0
36	38.76499817453884	40.0	38.0	41.0	35.0	41.0
37	38.69303100121216	40.0	38.0	41.0	35.0	41.0
38	38.61155974139644	40.0	38.0	41.0	35.0	41.0
39	38.506601568865264	40.0	38.0	41.0	35.0	41.0
40	38.388511764426816	40.0	38.0	41.0	35.0	41.0
41	38.27304154832109	40.0	38.0	41.0	35.0	41.0
42	38.125292641019456	40.0	37.0	41.0	34.0	41.0
43	37.28397850149921	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	3.0
13	5.0
14	6.0
15	3.0
16	6.0
17	12.0
18	30.0
19	35.0
20	83.0
21	160.0
22	299.0
23	512.0
24	799.0
25	1298.0
26	2009.0
27	2920.0
28	4347.0
29	6072.0
30	7841.0
31	10049.0
32	12785.0
33	17080.0
34	26232.0
35	38310.0
36	64313.0
37	132500.0
38	287685.0
39	839027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47622809532552	16.574293519028455	13.821800612887412	26.127677772758616
2	20.03538163139161	19.565574621483236	33.4490490069285	26.949994740196654
3	19.693322524953125	21.498294517359756	29.25323856061528	29.555144397071835
4	14.411173609950861	16.379027617061563	32.58864143748706	36.62115733550051
5	16.375727348295925	34.79060826016019	31.60247987695498	17.231184514588907
6	33.80155896445817	33.10932759086568	16.646280631478927	16.442832813197224
7	30.54288733638746	29.52145415342262	19.649937741804848	20.285720768385076
8	29.790082279825665	30.58317811756795	18.8177199680699	20.809019634536487
9	28.189108150495006	13.962268302224862	18.22126514428019	39.627358402999945
10	19.831933813109906	24.377641504179998	28.145310833751026	27.645113848959074
11	37.37134967928951	21.620816995284052	18.808506717765827	22.19932660766061
12	24.443096834698473	25.606854108181437	25.710881329898307	24.239167727221787
13	32.0854879619259	17.58163180414005	24.235386169261158	26.097494064672894
14	24.427420558061698	19.666232818835184	23.205496047584376	32.70085057551874
15	28.171025427883283	25.045670906824473	20.58357002448387	26.199733640808375
16	26.24779380470797	24.46839889523503	22.987815820250862	26.295991479806137
17	24.603434892229036	25.74140881598045	23.393198833630013	26.261957458160502
18	25.897690293153246	22.264575671381237	25.433039953191187	26.404694082274325
19	25.867919118663224	24.78054931598492	24.69735504085114	24.654176524500716
20	27.16238078638529	22.148447464190365	24.97093357040264	25.718238179021704
21	29.806927401650274	22.28417101717721	23.77926152361033	24.129640057562188
22	28.58080879961662	22.25556868787502	22.779967643614977	26.383654868893387
23	26.117570699663855	22.780998977604238	23.778367700819636	27.32306262191227
24	25.198239581635928	23.886657769692118	25.089536979167743	25.825565669504208
25	25.282740213156107	22.690241586549202	24.46035449011879	27.5666637101759
26	26.627324712756295	23.320799187583837	24.332469075450334	25.719407024209534
27	26.68755461772918	22.53973557971627	23.800919537384825	26.97179026516972
28	25.116764196484255	23.070391294991087	25.58437102721553	26.228473481309134
29	24.84786104768407	23.358477255991534	26.228404725709847	25.56525697061455
30	24.34175108135369	23.91154729663297	26.543992926423947	25.202708695589397
31	25.55700629870045	23.599946920677354	24.42425780049463	26.418788980127566
32	24.254981515057132	22.804513392559407	26.218641430611505	26.721863661771955
33	24.34828286328568	22.06655954544298	27.133090901090256	26.45206669018108
34	25.493269858164076	21.9526315174292	26.028738465388773	26.525360159017954
35	24.723688435376957	21.84523527134741	26.911422848998267	26.51965344427737
36	22.731907479715378	22.04235757449497	27.594647239084537	27.631087706705117
37	23.34802640490035	20.90844023110132	28.419920697291783	27.32361266670654
38	22.42993288765954	19.57987578613433	30.19388391442128	27.79630741178485
39	22.65772018808782	19.41919395060735	31.08798172751193	26.835104133792896
40	21.79861897503278	20.390710568491922	30.71711402497341	27.09355643150189
41	19.946068107921537	21.02346834870365	32.62157536954416	26.408888173830658
42	20.30517860298248	21.30646639535707	31.70251927391337	26.685835727747076
43	18.6069153006648	21.50833283485524	32.04794740471677	27.836804459763194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	61.0
2	85.0
3	436.0
4	787.0
5	787.0
6	1132.0
7	1477.0
8	1515.0
9	1553.0
10	2274.0
11	2995.0
12	2995.0
13	4862.0
14	6729.0
15	10713.0
16	14697.0
17	12919.0
18	11141.0
19	11141.0
20	11611.0
21	12081.0
22	9206.5
23	6332.0
24	6680.5
25	7029.0
26	7029.0
27	7904.5
28	8780.0
29	10587.0
30	12394.0
31	15122.0
32	17850.0
33	17850.0
34	22159.0
35	26468.0
36	32846.0
37	39224.0
38	46814.5
39	54405.0
40	54405.0
41	61085.0
42	67765.0
43	77637.5
44	87510.0
45	97412.0
46	107314.0
47	107314.0
48	118005.5
49	128697.0
50	127031.5
51	125366.0
52	121231.5
53	117097.0
54	117097.0
55	109497.5
56	101898.0
57	99102.0
58	96306.0
59	93896.0
60	91486.0
61	91486.0
62	85244.0
63	79002.0
64	73066.5
65	67131.0
66	60227.5
67	53324.0
68	53324.0
69	45817.5
70	38311.0
71	32786.0
72	27261.0
73	21148.5
74	15036.0
75	15036.0
76	12157.0
77	9278.0
78	7718.5
79	6159.0
80	5403.0
81	4647.0
82	4647.0
83	3683.0
84	2719.0
85	2374.5
86	2030.0
87	1667.5
88	1305.0
89	1305.0
90	943.5
91	582.0
92	332.5
93	83.0
94	57.5
95	32.0
96	32.0
97	26.5
98	21.0
99	12.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1454427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.560053734469996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59404635997358	49.22289976314258
2	6.3348836311846055	7.039329499004622
3	1.7792149568760578	2.9655983582761944
4	0.8641151941589726	1.9204114648097796
5	0.4695776426480757	1.3044879529016418
6	0.31773722254085135	1.0592098294686572
7	0.21955900499741496	0.8539097080880199
8	0.16027662266069545	0.7123982213926088
9	0.13378105073620516	0.6689594130801674
>10	0.8823540328468474	9.759640823283249
>50	0.11969654888600512	4.6971295165638205
>100	0.10972222463401476	12.187773866465236
>500	0.01267456034197493	4.810233215644137
>1k	0.00198816632815293	1.5653244782601765
>5k	3.727811865286744E-4	1.2326938896191706
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7527	0.5175233958115464	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5273	0.36254827502514736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5055	0.34755955438120995	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2223	0.15284369720859142	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1963	0.13496724139472108	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.1267853250799112	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1646	0.11317171642165609	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1606	0.11042149245029141	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1598	0.1098714476560185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.87555992841167E-5
2	0.0	0.0	0.0	6.87555992841167E-5	6.87555992841167E-5
3	0.0	0.0	0.0	6.87555992841167E-5	6.87555992841167E-5
4	0.0	6.87555992841167E-5	0.0	6.87555992841167E-5	6.87555992841167E-5
5	0.0	6.87555992841167E-5	0.0	6.87555992841167E-5	6.87555992841167E-5
6	0.0	6.87555992841167E-5	0.0	6.87555992841167E-5	1.375111985682334E-4
7	0.0	6.87555992841167E-5	0.0	6.87555992841167E-5	1.375111985682334E-4
8	0.0	6.87555992841167E-5	0.0	6.87555992841167E-5	1.375111985682334E-4
9	0.0	6.87555992841167E-5	0.0	1.375111985682334E-4	1.375111985682334E-4
10	0.0	6.87555992841167E-5	0.0	2.062667978523501E-4	1.375111985682334E-4
11	0.0	6.87555992841167E-5	0.0	5.500447942729336E-4	1.375111985682334E-4
12	0.0	6.87555992841167E-5	0.0	0.0010313339892617506	1.375111985682334E-4
13	0.0	6.87555992841167E-5	0.0	0.0013063563863982173	1.375111985682334E-4
14	0.0	6.87555992841167E-5	0.0	0.0022001791770917345	1.375111985682334E-4
15	0.0	6.87555992841167E-5	0.0	0.0033690243649217183	1.375111985682334E-4
16	0.0	6.87555992841167E-5	0.0	0.00666929313055932	2.062667978523501E-4
17	0.0	6.87555992841167E-5	0.0	0.010450851091185738	2.062667978523501E-4
18	0.0	6.87555992841167E-5	0.0	0.011963474275436306	2.062667978523501E-4
19	0.0	6.87555992841167E-5	0.0	0.01395738665467569	2.062667978523501E-4
20	0.0	6.87555992841167E-5	0.0	0.01540125423964214	2.062667978523501E-4
21	0.0	6.87555992841167E-5	0.0	0.01980161259382561	3.437779964205835E-4
22	0.0	6.87555992841167E-5	0.0	0.027433484114362562	4.812891949888169E-4
23	0.0	6.87555992841167E-5	0.0	0.043109760751141174	4.812891949888169E-4
24	0.0	6.87555992841167E-5	0.0	0.06854933248626435	4.812891949888169E-4
25	0.0	6.87555992841167E-5	0.0	0.0770062711982107	4.812891949888169E-4
26	0.0	6.87555992841167E-5	0.0	0.09770170658272984	4.812891949888169E-4
27	0.0	6.87555992841167E-5	0.0	0.18337118329073923	4.812891949888169E-4
28	0.0	6.87555992841167E-5	0.0	0.5642084477254616	4.812891949888169E-4
29	0.0	6.87555992841167E-5	0.0	1.141686726112758	4.812891949888169E-4
30	0.0	6.87555992841167E-5	0.0	2.0185268837830983	4.812891949888169E-4
31	6.87555992841167E-5	6.87555992841167E-5	0.0	3.7985405936495953	4.812891949888169E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	25	0.005496817	29.6	2
ATACACA	1060	0.0	23.386793	37
GCAGTCG	1880	0.0	20.664892	9
AGTCGGT	1885	0.0	20.61008	11
CGTGATA	45	0.003826289	20.555555	2
GTTAGCC	45	0.003826289	20.555555	9
CAGTCGG	1910	0.0	20.437174	10
GGCAGTC	1965	0.0	19.865139	8
GGGCAGT	2060	0.0	19.12864	7
TCGGTGA	2100	0.0	18.67619	13
CGGTGAT	2130	0.0	18.413145	14
TATTGAC	375	0.0	18.253332	30
GTCGGTG	2160	0.0	18.243055	12
GTGATTC	2175	0.0	18.11724	16
GGTATCA	5350	0.0	17.91215	1
GGTGATT	2210	0.0	17.830317	15
TATTAGA	125	8.583811E-9	17.76	2
ATTGACT	375	0.0	17.759998	31
TATACAC	1940	0.0	17.641752	37
GATTCCT	2190	0.0	17.570776	18
>>END_MODULE