FastQCFastQC Report
Fri 10 Feb 2017
ERR1631347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32617
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5211.5973265475059018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4331.327528589385903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3601.1037189195818131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.47521231259772506No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1340.41082870895545265No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA810.24833675690590795No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.24220498513045347No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA600.18395315326363554No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.17475549560045375No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT560.17168960971272648No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.16555783793727197No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC540.16555783793727197No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA520.15942606616181745No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA510.15636018027409018No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT480.14716252261090843No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA480.14716252261090843No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.14409663672318118No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA470.14409663672318118No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC450.13796486494772664No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.12876720728454488No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT390.11956954962136308No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT370.11343777784590858No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG340.10424012018272681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC250.005419329329.630
CTTATAC400.001891445323.12500237
GACTGTC400.001891445323.12500234
CAGTCGG1001.1785232E-820.3510
AGTCGGT1001.1785232E-820.3511
GCAGTCG1101.5770638E-920.1818189
GGGCAGT1152.743036E-919.3043487
TGGGCAG1152.743036E-919.3043486
GGCAGTC1152.743036E-919.3043488
TCTTATA701.1737564E-418.537
GGTATCA2650.018.1509441
GCCTTAT1005.533926E-616.65000226
GTGATTC1251.5177648E-716.27999916
CCTTATT1058.799463E-615.85714427
TGATTCC1403.130117E-815.85714317
GGTGATT1302.3713801E-715.65384615
TTATTGA1302.3713801E-715.65384629
CTTATTG1302.3713801E-715.65384628
GATTCCT1302.3713801E-715.65384618
CGGTGAT1353.6398342E-715.07407514
Produced by FastQC (version 0.11.5)