##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32617
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89557592666401	34.0	33.0	34.0	31.0	34.0
2	32.99914155195144	34.0	33.0	34.0	31.0	34.0
3	33.02750099641291	34.0	33.0	34.0	31.0	34.0
4	36.376981328754944	37.0	37.0	37.0	35.0	37.0
5	36.35837140141644	37.0	37.0	37.0	35.0	37.0
6	36.43468130116197	37.0	37.0	37.0	35.0	37.0
7	36.42548364349879	37.0	37.0	37.0	35.0	37.0
8	36.44574914921667	37.0	37.0	37.0	35.0	37.0
9	38.2673759082687	39.0	39.0	39.0	37.0	39.0
10	38.211484808535424	39.0	39.0	39.0	37.0	39.0
11	38.281172394763466	39.0	39.0	39.0	37.0	39.0
12	38.23245546800748	39.0	39.0	39.0	37.0	39.0
13	38.27878100377104	39.0	39.0	39.0	37.0	39.0
14	39.6974277217402	41.0	40.0	41.0	37.0	41.0
15	39.63656988686881	41.0	40.0	41.0	37.0	41.0
16	39.58858877272588	41.0	39.0	41.0	37.0	41.0
17	39.597295888647025	41.0	39.0	41.0	37.0	41.0
18	39.645430297084346	41.0	40.0	41.0	37.0	41.0
19	39.67795934635313	41.0	40.0	41.0	37.0	41.0
20	39.682864763773495	41.0	40.0	41.0	37.0	41.0
21	39.64883343041972	41.0	40.0	41.0	37.0	41.0
22	39.56492013367262	41.0	40.0	41.0	37.0	41.0
23	39.460588036913265	41.0	39.0	41.0	36.0	41.0
24	39.41558083208143	41.0	39.0	41.0	36.0	41.0
25	39.36830487169268	41.0	39.0	41.0	36.0	41.0
26	39.18919581813165	41.0	39.0	41.0	35.0	41.0
27	39.059999386822824	41.0	39.0	41.0	35.0	41.0
28	38.943250452218166	40.0	38.0	41.0	35.0	41.0
29	38.78060520587424	40.0	38.0	41.0	35.0	41.0
30	38.576969065211394	40.0	38.0	41.0	35.0	41.0
31	38.3595057791949	40.0	37.0	41.0	35.0	41.0
32	38.18931845356716	40.0	37.0	41.0	35.0	41.0
33	37.95303062820002	40.0	36.0	41.0	35.0	41.0
34	37.789864181255176	40.0	35.0	41.0	34.0	41.0
35	37.56222215409142	40.0	35.0	41.0	33.0	41.0
36	37.36232639421161	40.0	35.0	41.0	33.0	41.0
37	37.17975288959745	40.0	35.0	41.0	33.0	41.0
38	36.924058006561	39.0	35.0	41.0	33.0	41.0
39	36.66385627126958	39.0	35.0	41.0	33.0	41.0
40	36.35910721402949	39.0	35.0	41.0	32.0	41.0
41	36.06787871355428	39.0	35.0	41.0	31.0	41.0
42	35.75908268694239	38.0	35.0	41.0	30.0	41.0
43	34.713492963791886	37.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	1.0
19	2.0
20	8.0
21	7.0
22	9.0
23	29.0
24	46.0
25	63.0
26	65.0
27	142.0
28	198.0
29	254.0
30	383.0
31	436.0
32	497.0
33	661.0
34	852.0
35	1274.0
36	2122.0
37	4969.0
38	7187.0
39	13409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.086396664316155	15.703467516939021	13.768893521783118	24.441242296961708
2	20.593555507863996	20.118343195266274	31.55103167060122	27.73706962626851
3	21.84443695005672	21.18220559830763	28.85305208940123	28.120305362234415
4	15.632952141521292	16.549652021951744	29.70536836618941	38.11202747033756
5	16.310512922709016	36.14986050219211	28.862249747064418	18.67737682803446
6	32.17953827758531	30.793757856332586	17.757611061716283	19.269092804365823
7	30.64352944783395	30.336940859061222	17.82506055124628	21.19446914185854
8	33.84738020050894	27.890363920654877	17.239476346690378	21.02277953214581
9	29.9291780359935	12.0458656528804	17.282398749118556	40.74255756200754
10	22.154091424717173	24.803016831713524	24.4627034981758	28.580188245393508
11	37.05429683907165	22.865376950669898	16.29518349327038	23.785142716988073
12	24.064138332771254	27.592972989545327	23.93537112548671	24.40751755219671
13	35.31900542661802	17.561394364901737	23.585860134285802	23.53374007419444
14	25.27516325842352	19.670723855658093	24.177576110617164	30.876536775301222
15	31.931201520679398	23.26087623018671	20.035564276297634	24.77235797283625
16	26.34209154735261	23.595057791948985	23.726890885121254	26.335959775577155
17	22.97268295674035	27.080970046294876	23.8893828371708	26.05696415979397
18	24.62519545022534	21.274182174939448	27.041113529754423	27.05950884508079
19	25.183186681791703	25.48364349878898	27.629763620198055	21.703406199221263
20	26.771315571634425	21.108624337002176	28.592451788944416	23.527608302418983
21	29.2884078854585	20.42186589815127	26.68853665266579	23.60118956372444
22	27.847441518226688	21.510255388294446	24.530152987705797	26.112150105773065
23	25.327283318514887	22.78259803170126	25.670662537940338	26.21945611184352
24	24.6313272220008	22.997210043842166	27.614434190759418	24.757028543397613
25	24.17144433884171	23.168899653554895	25.57562007542079	27.084035932182605
26	26.16427016586443	22.310451604991265	26.259312628383974	25.26596560076034
27	25.014562957966703	22.482141214703987	25.719716712143974	26.783579115185336
28	23.861789864181254	22.62317196553944	27.418217493944873	26.096820676334424
29	23.895514608946257	23.77901094521262	27.991538154949875	24.333936290891252
30	23.30686451850262	23.97829352791489	29.14431124873532	23.570530704847165
31	23.582794248398077	24.533218873593523	26.893951007143514	24.990035870864887
32	23.23328325719717	24.597602477235796	28.715087224453505	23.45402704111353
33	23.6533096238158	23.21795382775853	29.015544041450774	24.11319250697489
34	24.833675690590795	23.604255449612165	25.753441456908977	25.808627402888064
35	23.484685899990804	24.947113468436704	26.59656007603397	24.971640555538524
36	21.905754667811266	24.57614127602171	27.911825121868965	25.606278934298064
37	22.414691725173988	24.17451022472944	28.206150167090783	25.204647883005794
38	22.558788361897168	22.273660974338537	29.74829076861759	25.419259895146702
39	23.15050433822853	21.902688781923537	29.573535273017136	25.373271606830794
40	22.4606800134899	21.73099917221081	28.761075512769413	27.047245301529877
41	20.03249839040991	22.564920133672626	30.57914584419168	26.82343563172579
42	20.593555507863996	22.013060673881718	29.858662660575774	27.53472115767851
43	19.04834902044946	23.588926020173528	31.088082901554404	26.274642057822607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	33.5
4	63.0
5	63.0
6	76.0
7	89.0
8	96.0
9	103.0
10	158.5
11	214.0
12	214.0
13	380.5
14	547.0
15	784.0
16	1021.0
17	912.0
18	803.0
19	803.0
20	861.0
21	919.0
22	662.5
23	406.0
24	343.0
25	280.0
26	280.0
27	293.0
28	306.0
29	312.0
30	318.0
31	325.0
32	332.0
33	332.0
34	346.5
35	361.0
36	400.0
37	439.0
38	520.5
39	602.0
40	602.0
41	684.5
42	767.0
43	940.5
44	1114.0
45	1384.5
46	1655.0
47	1655.0
48	2112.5
49	2570.0
50	2604.5
51	2639.0
52	2246.5
53	1854.0
54	1854.0
55	1763.5
56	1673.0
57	1736.0
58	1799.0
59	1794.0
60	1789.0
61	1789.0
62	1820.0
63	1851.0
64	1883.0
65	1915.0
66	1849.5
67	1784.0
68	1784.0
69	1634.0
70	1484.0
71	1315.5
72	1147.0
73	973.5
74	800.0
75	800.0
76	638.5
77	477.0
78	378.0
79	279.0
80	202.5
81	126.0
82	126.0
83	89.0
84	52.0
85	36.0
86	20.0
87	14.5
88	9.0
89	9.0
90	6.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	32617.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.09679001747556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.76720027908058	74.16684551001012
2	3.6047908833675724	5.702547751172701
3	0.9729059265863017	2.308612073458626
4	0.43024923446645214	1.361253334150903
5	0.29070894220706234	1.1497072078977222
6	0.17054924609480987	0.8093938743599963
7	0.1317880538005349	0.7296808412790876
8	0.09690298073568743	0.6131771775454518
9	0.05426566921198496	0.3863016218536346
>10	0.4263731152370247	6.1900236073213355
>50	0.03488507306484748	1.667841922923629
>100	0.015504476917709988	3.317288530520894
>500	0.003876119229427497	1.5973265475059018
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	1.5973265475059018	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	433	1.327528589385903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	360	1.1037189195818131	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.47521231259772506	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	134	0.41082870895545265	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	81	0.24833675690590795	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.24220498513045347	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	60	0.18395315326363554	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.17475549560045375	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	56	0.17168960971272648	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.16555783793727197	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	54	0.16555783793727197	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	52	0.15942606616181745	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	51	0.15636018027409018	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	48	0.14716252261090843	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	48	0.14716252261090843	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.14409663672318118	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	47	0.14409663672318118	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	45	0.13796486494772664	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42	0.12876720728454488	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	39	0.11956954962136308	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	37	0.11343777784590858	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	34	0.10424012018272681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.003065885887727259
17	0.0	0.0	0.0	0.0	0.003065885887727259
18	0.0	0.0	0.0	0.0	0.003065885887727259
19	0.0	0.0	0.0	0.003065885887727259	0.003065885887727259
20	0.0	0.0	0.0	0.003065885887727259	0.003065885887727259
21	0.0	0.0	0.0	0.006131771775454518	0.003065885887727259
22	0.0	0.0	0.0	0.018395315326363553	0.003065885887727259
23	0.0	0.0	0.0	0.02452708710181807	0.003065885887727259
24	0.0	0.0	0.0	0.03985651654045436	0.003065885887727259
25	0.0	0.0	0.0	0.0521200600913634	0.003065885887727259
26	0.0	0.0	0.0	0.07358126130545421	0.003065885887727259
27	0.0	0.0	0.0	0.17168960971272648	0.003065885887727259
28	0.0	0.0	0.0	0.3709721924149983	0.003065885887727259
29	0.0	0.0	0.0	0.6836925529631787	0.003065885887727259
30	0.0	0.0	0.0	1.0975871478063586	0.003065885887727259
31	0.0	0.0	0.0	1.7230278689027194	0.003065885887727259
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGAC	25	0.0054193293	29.6	30
CTTATAC	40	0.0018914453	23.125002	37
GACTGTC	40	0.0018914453	23.125002	34
CAGTCGG	100	1.1785232E-8	20.35	10
AGTCGGT	100	1.1785232E-8	20.35	11
GCAGTCG	110	1.5770638E-9	20.181818	9
GGGCAGT	115	2.743036E-9	19.304348	7
TGGGCAG	115	2.743036E-9	19.304348	6
GGCAGTC	115	2.743036E-9	19.304348	8
TCTTATA	70	1.1737564E-4	18.5	37
GGTATCA	265	0.0	18.150944	1
GCCTTAT	100	5.533926E-6	16.650002	26
GTGATTC	125	1.5177648E-7	16.279999	16
CCTTATT	105	8.799463E-6	15.857144	27
TGATTCC	140	3.130117E-8	15.857143	17
GGTGATT	130	2.3713801E-7	15.653846	15
TTATTGA	130	2.3713801E-7	15.653846	29
CTTATTG	130	2.3713801E-7	15.653846	28
GATTCCT	130	2.3713801E-7	15.653846	18
CGGTGAT	135	3.6398342E-7	15.074075	14
>>END_MODULE