FastQCFastQC Report
Fri 10 Feb 2017
ERR1631346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141946
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18521.304721513815113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13570.9559973511053499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11770.8291885646654361No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.38183534583574036No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2280.16062446282389076No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1680.11835486734391952No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.11624138756992095No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.11624138756992095No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1560.10990094824792528No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1550.10919645498992574No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.10849196173192623No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1490.10496949544192861No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1460.10285601566793005No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1440.10144702915193102No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.10003804263593197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC303.583738E-430.8333348
GTCCTAT250.00548032229.61
CAAGACG453.9757997E-628.7777774
GAGCGAA358.830976E-426.42857216
CGCAAGA358.830976E-426.4285722
CGAAAGC400.001922901823.12519
AAGACGG400.001922901823.1255
GACCAGA400.001922901823.12511
CGTCAGA400.001922901823.1251
AGAGCGA400.001922901823.12515
CGGACCA400.001922901823.1259
CGCTTAT603.696743E-521.58333426
GCAGTCG1550.021.4838719
AGTCGGT1550.021.48387111
CGGTGAT1700.020.67647214
TCGGTGA1700.020.67647213
CGGGTAA450.003809551720.55555524
GCGAAAG450.003809551720.55555518
ATCAGGG450.003809551720.55555529
ATTAAGA450.003809551720.5555552
Produced by FastQC (version 0.11.5)