##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141946
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78012765417835	34.0	31.0	34.0	31.0	34.0
2	32.92635931974131	34.0	33.0	34.0	31.0	34.0
3	33.01633719865301	34.0	33.0	34.0	31.0	34.0
4	36.38422357798036	37.0	37.0	37.0	35.0	37.0
5	36.36721006579967	37.0	37.0	37.0	35.0	37.0
6	36.424034491989914	37.0	37.0	37.0	35.0	37.0
7	36.42509827680949	37.0	37.0	37.0	35.0	37.0
8	36.43299564623167	37.0	37.0	37.0	35.0	37.0
9	38.26516421737844	39.0	39.0	39.0	37.0	39.0
10	38.23139081058994	39.0	39.0	39.0	37.0	39.0
11	38.289955335127445	39.0	39.0	39.0	37.0	39.0
12	38.094423231369674	39.0	39.0	39.0	37.0	39.0
13	38.21756865286799	39.0	39.0	39.0	37.0	39.0
14	39.68221718118158	41.0	40.0	41.0	37.0	41.0
15	39.65963817226269	41.0	40.0	41.0	37.0	41.0
16	39.605758527890885	41.0	39.0	41.0	37.0	41.0
17	39.63146548687529	41.0	40.0	41.0	37.0	41.0
18	39.66595747678695	41.0	40.0	41.0	37.0	41.0
19	39.69067110027757	41.0	40.0	41.0	37.0	41.0
20	39.66030039592521	41.0	40.0	41.0	37.0	41.0
21	39.63295901258225	41.0	40.0	41.0	37.0	41.0
22	39.55512659743846	41.0	40.0	41.0	37.0	41.0
23	39.47353923322954	41.0	39.0	41.0	36.0	41.0
24	39.43253068068139	41.0	39.0	41.0	36.0	41.0
25	39.43461598072506	41.0	39.0	41.0	36.0	41.0
26	39.32732165753174	41.0	39.0	41.0	36.0	41.0
27	39.23000295887169	41.0	39.0	41.0	35.0	41.0
28	39.10992912797825	41.0	39.0	41.0	35.0	41.0
29	38.99448381778986	40.0	39.0	41.0	35.0	41.0
30	38.876227579502064	40.0	38.0	41.0	35.0	41.0
31	38.75182816000451	40.0	38.0	41.0	35.0	41.0
32	38.626534034069294	40.0	38.0	41.0	35.0	41.0
33	38.48878446733265	40.0	38.0	41.0	35.0	41.0
34	38.409789638313164	40.0	38.0	41.0	35.0	41.0
35	38.254477054654586	40.0	37.0	41.0	35.0	41.0
36	38.166344948078844	40.0	37.0	41.0	35.0	41.0
37	38.03956434136925	40.0	37.0	41.0	34.0	41.0
38	37.89092330886393	40.0	37.0	41.0	34.0	41.0
39	37.72286644216815	40.0	37.0	41.0	34.0	41.0
40	37.5579657052682	40.0	36.0	41.0	33.0	41.0
41	37.35350767193158	40.0	36.0	41.0	33.0	41.0
42	37.133649415975086	40.0	35.0	41.0	33.0	41.0
43	36.21751229340735	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	2.0
16	2.0
17	3.0
18	3.0
19	10.0
20	15.0
21	26.0
22	42.0
23	84.0
24	117.0
25	177.0
26	297.0
27	449.0
28	655.0
29	905.0
30	1221.0
31	1586.0
32	2036.0
33	2466.0
34	3601.0
35	4892.0
36	7888.0
37	16359.0
38	27581.0
39	71526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58840685894636	17.91526355092782	14.073661815056429	24.422667775069392
2	18.860693503163176	21.78011356431319	33.63814408296113	25.72104884956251
3	19.545460949938708	23.527256844152	30.358727966973355	26.56855423893593
4	13.863722824172573	16.947289814436477	33.634621616671126	35.554365744719824
5	14.363208544094233	37.370549363842585	32.41091682752596	15.855325264537218
6	31.51127893706057	34.549758358812504	17.580629253378042	16.358333450748876
7	28.569315091654573	31.616248432502502	20.566271680780016	19.248164795062912
8	29.858537753793694	30.875826018345006	19.685655108280613	19.579981119580687
9	28.121961872824876	14.126498809406392	19.813872881236527	37.937666436532204
10	18.92902934918913	26.934890733095685	29.464021529313968	24.672058388401222
11	35.147168641596096	23.535006269989996	19.553210375776704	21.7646147126372
12	22.58464486494864	27.88102517858904	27.46819212940132	22.066137827060995
13	31.00193031152692	19.137559353556988	25.919011455060375	23.94149887985572
14	23.214461837600215	21.48281740943739	25.778817296718472	29.52390345624392
15	26.77637975004579	27.344201315993406	22.43951925380074	23.43989968016006
16	23.831597931607796	26.53192058951996	25.28214954982881	24.35433192904344
17	22.368365434742792	27.815507305595084	26.040888788694293	23.77523847096783
18	23.013681259070353	23.98306398207769	28.666535161258505	24.33671959759345
19	23.083426091612303	26.685500119763855	28.559452186042577	21.67162160258126
20	24.45859693122737	24.062671720231638	28.578473504008567	22.90025784453243
21	26.091612303270256	24.47127780987136	27.605568314711228	21.831541572147152
22	25.458977357586686	24.418440815521393	26.612937314189907	23.509644512702014
23	23.294069575754158	25.332168571146774	27.216688036295494	24.157073816803575
24	22.41204401673876	25.967621489862342	28.34105927606273	23.27927521733617
25	22.685387400842576	25.094754343200936	27.410423682245362	24.80943457371113
26	23.357473968974116	25.92112493483437	27.640088484353203	23.081312611838307
27	23.88020796640976	24.77773237710115	27.268820537387455	24.07323911910163
28	22.14433657869894	25.699914051822525	28.741915939864455	23.41383342961408
29	21.857607822693137	25.979597875248334	29.327349837262055	22.835444464796474
30	21.532131937497358	26.94404914544968	29.63591788426585	21.887901032787116
31	22.687500880616575	26.805263973623774	27.453397770983333	23.05383737477632
32	21.670212616065264	26.180378453778197	28.797570907246417	23.351838022910123
33	21.739252955349215	25.611852394572583	29.16249841489017	23.486396235188028
34	22.91082524340242	25.430797627266706	28.183957279528837	23.474419849802036
35	22.080932185478986	25.7323207416905	28.963831316134304	23.222915756696207
36	20.28024741803221	26.016231524664306	29.378073351838026	24.325447705465457
37	21.494089301565385	25.060938666816956	29.61266960675186	23.832302424865794
38	20.784664590759867	23.843574316993788	30.7377453397771	24.63401575246925
39	21.14677412537162	23.659701576655912	31.197779437250784	23.995744860721683
40	20.355628196638158	23.85273272934778	31.08717399574486	24.7044650782692
41	18.95791357276711	24.269088244825497	32.937173291251604	23.83582489115579
42	19.50107787468474	24.03096952362166	32.27706310850605	24.19088949318755
43	18.17522156312964	23.98658644836769	32.654671494793796	25.183520493708876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	26.0
2	26.0
3	97.5
4	169.0
5	169.0
6	254.5
7	340.0
8	350.0
9	360.0
10	546.5
11	733.0
12	733.0
13	1227.0
14	1721.0
15	2662.5
16	3604.0
17	3156.5
18	2709.0
19	2709.0
20	2988.5
21	3268.0
22	2543.5
23	1819.0
24	1856.0
25	1893.0
26	1893.0
27	2013.0
28	2133.0
29	2415.5
30	2698.0
31	3007.0
32	3316.0
33	3316.0
34	3702.0
35	4088.0
36	4614.5
37	5141.0
38	5594.0
39	6047.0
40	6047.0
41	6461.0
42	6875.0
43	7360.0
44	7845.0
45	8628.5
46	9412.0
47	9412.0
48	10437.0
49	11462.0
50	11102.5
51	10743.0
52	10464.5
53	10186.0
54	10186.0
55	9195.0
56	8204.0
57	7822.5
58	7441.0
59	7034.0
60	6627.0
61	6627.0
62	6210.0
63	5793.0
64	5378.5
65	4964.0
66	4465.5
67	3967.0
68	3967.0
69	3406.0
70	2845.0
71	2496.5
72	2148.0
73	1648.0
74	1148.0
75	1148.0
76	912.0
77	676.0
78	583.0
79	490.0
80	434.0
81	378.0
82	378.0
83	318.5
84	259.0
85	214.0
86	169.0
87	156.0
88	143.0
89	143.0
90	104.0
91	65.0
92	36.5
93	8.0
94	6.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.51684443379877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.85449808299265	60.433545150972904
2	4.308500497786439	5.731757147084102
3	1.4689995551695652	2.9313964465360067
4	0.7710394204494906	2.0514843672946053
5	0.5041411595246669	1.67669395403886
6	0.36751466881315004	1.4667549631550025
7	0.2478340994301934	1.1539599566032153
8	0.17157745345167233	0.9130232623673792
9	0.16098625262132218	0.9637467769433447
>10	1.030523840793069	13.128231862821071
>50	0.09108432714101125	4.24950333225311
>100	0.01906416149463026	1.8281600045087567
>500	0.0010591200830350144	0.38183534583574036
>1k	0.003177360249105043	3.089907429585899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1852	1.304721513815113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1357	0.9559973511053499	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1177	0.8291885646654361	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.38183534583574036	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	228	0.16062446282389076	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	168	0.11835486734391952	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.11624138756992095	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.11624138756992095	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	156	0.10990094824792528	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	155	0.10919645498992574	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	154	0.10849196173192623	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	149	0.10496949544192861	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	146	0.10285601566793005	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	144	0.10144702915193102	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.10003804263593197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.04493257999521E-4	0.0
10	0.0	0.0	0.0	7.04493257999521E-4	0.0
11	0.0	0.0	0.0	7.04493257999521E-4	0.0
12	0.0	0.0	0.0	0.002113479773998563	0.0
13	0.0	0.0	0.0	0.002113479773998563	0.0
14	0.0	0.0	0.0	0.002817973031998084	0.0
15	0.0	0.0	0.0	0.004931452805996646	0.0
16	0.0	0.0	0.0	0.007044932579995209	0.0
17	0.0	0.0	0.0	0.011976385385991856	0.0
18	0.0	0.0	0.0	0.015498851675989462	0.0
19	0.0	0.0	0.0	0.018316824707987546	0.0
20	0.0	0.0	0.0	0.02113479773998563	0.0
21	0.0	0.0	0.0	0.025361757287982754	0.0
22	0.0	0.0	0.0	0.03522466289997605	0.0
23	0.0	0.0	0.0	0.04508756851196934	0.0
24	0.0	0.0	0.0	0.061995406703957846	0.0
25	0.0	0.0	0.0	0.07115381905795161	0.0
26	0.0	0.0	0.0	0.08524368421794204	0.0
27	0.0	0.0	0.0	0.1211728403759176	0.0
28	0.0	0.0	0.0	0.29306919532780074	0.0
29	0.0	0.0	0.0	0.5727530187536105	0.0
30	0.0	0.0	0.0	0.9665647499753427	0.0
31	0.0	0.0	0.0	1.8182970988967635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACC	30	3.583738E-4	30.833334	8
GTCCTAT	25	0.005480322	29.6	1
CAAGACG	45	3.9757997E-6	28.777777	4
GAGCGAA	35	8.830976E-4	26.428572	16
CGCAAGA	35	8.830976E-4	26.428572	2
CGAAAGC	40	0.0019229018	23.125	19
AAGACGG	40	0.0019229018	23.125	5
GACCAGA	40	0.0019229018	23.125	11
CGTCAGA	40	0.0019229018	23.125	1
AGAGCGA	40	0.0019229018	23.125	15
CGGACCA	40	0.0019229018	23.125	9
CGCTTAT	60	3.696743E-5	21.583334	26
GCAGTCG	155	0.0	21.483871	9
AGTCGGT	155	0.0	21.483871	11
CGGTGAT	170	0.0	20.676472	14
TCGGTGA	170	0.0	20.676472	13
CGGGTAA	45	0.0038095517	20.555555	24
GCGAAAG	45	0.0038095517	20.555555	18
ATCAGGG	45	0.0038095517	20.555555	29
ATTAAGA	45	0.0038095517	20.555555	2
>>END_MODULE