FastQCFastQC Report
Fri 10 Feb 2017
ERR1631345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001068
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12710.1269644020186441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11810.11797400376398007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.11317912469482592No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10530.105187659579569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.025.2682911
ACTGATC2250.021.3777778
AACGGAG2000.021.27500225
GTCTAGA2300.020.9130441
ACAACGG2050.020.756123
TACCGAT450.003825467820.55555727
TAGAACT2350.020.4680844
ACGGAGT2000.020.3526
TCTAGCG2700.019.18518628
GTATCAA8200.019.176832
CTAGCGG2750.018.83636329
GTTCTAG2600.018.51
CAACGGA2300.018.524
TACATTG701.2189976E-418.55
TAGGCTC500.00703426218.55
GTCTTAC500.00703426218.51
TAGGATC609.2356786E-418.54
CTAGAAC2650.018.1509443
CGGAGTC2350.017.31914727
ATAATAC650.001579741117.0769233
Produced by FastQC (version 0.11.5)