##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001068
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85269332353047	34.0	33.0	34.0	31.0	34.0
2	33.01642445867813	34.0	33.0	34.0	31.0	34.0
3	33.136397327654066	34.0	33.0	34.0	31.0	34.0
4	36.45417494116284	37.0	37.0	37.0	35.0	37.0
5	36.4307919142356	37.0	37.0	37.0	35.0	37.0
6	36.473132694282505	37.0	37.0	37.0	35.0	37.0
7	36.460477210339356	37.0	37.0	37.0	35.0	37.0
8	36.449695724965736	37.0	37.0	37.0	35.0	37.0
9	38.30592227501029	39.0	39.0	39.0	37.0	39.0
10	38.26381624425114	39.0	39.0	39.0	37.0	39.0
11	38.32552933467057	39.0	39.0	39.0	37.0	39.0
12	38.10562519229463	39.0	39.0	39.0	37.0	39.0
13	38.234462593949665	39.0	39.0	39.0	37.0	39.0
14	39.772682774796515	41.0	40.0	41.0	38.0	41.0
15	39.77204046078788	41.0	40.0	41.0	38.0	41.0
16	39.761431790847375	41.0	40.0	41.0	38.0	41.0
17	39.748093036636874	41.0	40.0	41.0	38.0	41.0
18	39.72719535536047	41.0	40.0	41.0	38.0	41.0
19	39.73819161135907	41.0	40.0	41.0	38.0	41.0
20	39.701147174817294	41.0	40.0	41.0	38.0	41.0
21	39.681768870845936	41.0	40.0	41.0	37.0	41.0
22	39.6329759816516	41.0	40.0	41.0	37.0	41.0
23	39.57937123152473	41.0	40.0	41.0	37.0	41.0
24	39.545804081241236	41.0	40.0	41.0	37.0	41.0
25	39.538674695425286	41.0	39.0	41.0	37.0	41.0
26	39.472731123160465	41.0	39.0	41.0	37.0	41.0
27	39.41844310276625	41.0	39.0	41.0	37.0	41.0
28	39.349569659603546	41.0	39.0	41.0	36.0	41.0
29	39.29623362249118	41.0	39.0	41.0	36.0	41.0
30	39.254292415700036	41.0	39.0	41.0	36.0	41.0
31	39.217816372114584	41.0	39.0	41.0	36.0	41.0
32	39.14781313557121	41.0	39.0	41.0	35.0	41.0
33	39.06532723051781	40.0	39.0	41.0	35.0	41.0
34	39.03100488678092	40.0	39.0	41.0	35.0	41.0
35	38.948708779024	40.0	39.0	41.0	35.0	41.0
36	38.89851438663507	40.0	39.0	41.0	35.0	41.0
37	38.843183480043315	40.0	38.0	41.0	35.0	41.0
38	38.75835707464428	40.0	38.0	41.0	35.0	41.0
39	38.691643324928975	40.0	38.0	41.0	35.0	41.0
40	38.60987465386967	40.0	38.0	41.0	35.0	41.0
41	38.52962935584795	40.0	38.0	41.0	35.0	41.0
42	38.42098638653918	40.0	38.0	41.0	35.0	41.0
43	37.51950217168064	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	6.0
17	8.0
18	11.0
19	36.0
20	49.0
21	97.0
22	212.0
23	337.0
24	579.0
25	883.0
26	1393.0
27	2028.0
28	2799.0
29	3856.0
30	5451.0
31	7276.0
32	9730.0
33	13074.0
34	19680.0
35	28471.0
36	45747.0
37	85459.0
38	211047.0
39	562835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5656718624509	17.963215286074472	11.66853800141449	26.802574850060136
2	19.577890812612132	19.761394830321215	33.28385284516136	27.376861511905286
3	20.23898476427176	21.212944575193692	28.47428945885794	30.07378120167661
4	14.913672198092437	14.841349438799362	33.29024601725357	36.95473234585463
5	16.53753790951264	34.490264397623335	32.14766629239972	16.824531400464306
6	36.09654888578998	33.426600390782646	15.251711172467806	15.225139550959573
7	31.071715407944318	28.96057011112132	20.23139287241226	19.736321608522097
8	28.300774772542926	31.630718392756535	19.491882669309177	20.57662416539136
9	27.310332564820772	13.88197405171277	19.064539072270815	39.743154311195646
10	18.46547886856837	24.895611486931955	31.077709006780758	25.56120063771892
11	38.23606388377213	20.490116555518707	20.205320717473736	21.068498843235425
12	23.455549473162662	24.342002741072534	27.375063432254354	24.82738435351045
13	31.038051361146294	18.059212760771494	24.204349754462235	26.698386123619972
14	23.99337507541945	19.44423355855946	23.289926358648962	33.272465007372126
15	26.667419196298354	26.044084917308314	20.839543367683312	26.448952518710016
16	26.946920688704463	24.41182816751709	22.578486176763217	26.062764967015227
17	25.557204905161292	24.903902631989038	23.544054949314134	25.994837513535547
18	26.12679658125122	23.09703236942945	24.772642817470942	26.00352823184839
19	26.070856325444424	23.79398802079379	24.17268357394303	25.962472079818756
20	26.19252638182421	23.392317005438194	24.144213979469924	26.27094263326767
21	27.49153903630922	23.58391238157648	23.91036373153472	25.01418485057958
22	27.075083810490398	23.21900210575106	23.60279221791127	26.103121865847275
23	25.926011020230394	23.111916473206616	24.335309889038506	26.626762617524484
24	25.68127240107565	24.023243176287725	23.925547515253708	26.36993690738291
25	26.334075207678197	23.263354737140734	24.064099541689476	26.33847051349159
26	26.25985447542025	24.16429253557201	23.853024969332754	25.72282801967499
27	26.562431323346665	23.116511565647887	23.866210886772926	26.45484622423252
28	25.47908833365965	23.408200042354764	24.719999041024185	26.392712582961398
29	25.26961205432598	23.668022551914554	24.521710812851875	26.540654580907592
30	24.877231117166865	24.22362916405279	24.978722724130627	25.920416994649713
31	26.302409027158998	23.65923194028777	23.697590972841006	26.34076805971223
32	25.494571797320464	23.519381300770778	23.87070608590026	27.115340816008505
33	25.034363299995604	23.14098542756336	24.850060135774992	26.974591136666042
34	26.36624085476711	22.845201324984917	24.431806830305234	26.35675098994274
35	25.922814434184293	22.98485217787403	25.30427503426341	25.788058353678274
36	25.02826980784522	23.312702034227446	25.123168456088894	26.535859701838437
37	25.760487799030635	22.37120755033624	25.456512444709052	26.411792205924073
38	24.888419168328223	22.09720019019687	26.3173930242501	26.696987617224803
39	25.15992919561908	21.867945034702938	26.3362728605849	26.63585290909309
40	24.67434779655328	22.509759576772005	26.78329544046958	26.03259718620513
41	24.158298936735566	22.23775008291145	27.482049171484856	26.12190180886813
42	24.67854331573879	22.42844641922427	27.271573959011775	25.621436306025164
43	23.31180299440198	21.878633619294593	27.470761227009554	27.338802159293873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	147.5
2	124.0
3	269.0
4	414.0
5	414.0
6	453.0
7	492.0
8	437.5
9	383.0
10	541.0
11	699.0
12	699.0
13	1140.5
14	1582.0
15	2092.5
16	2603.0
17	2570.0
18	2537.0
19	2537.0
20	3017.0
21	3497.0
22	3453.0
23	3409.0
24	4147.5
25	4886.0
26	4886.0
27	6085.0
28	7284.0
29	9005.5
30	10727.0
31	13107.5
32	15488.0
33	15488.0
34	19362.0
35	23236.0
36	28650.0
37	34064.0
38	39241.0
39	44418.0
40	44418.0
41	48326.0
42	52234.0
43	55985.0
44	59736.0
45	63908.5
46	68081.0
47	68081.0
48	71926.0
49	75771.0
50	77512.5
51	79254.0
52	80950.0
53	82646.0
54	82646.0
55	80053.5
56	77461.0
57	74403.5
58	71346.0
59	69132.5
60	66919.0
61	66919.0
62	62348.5
63	57778.0
64	53008.5
65	48239.0
66	42828.5
67	37418.0
68	37418.0
69	31133.5
70	24849.0
71	21348.5
72	17848.0
73	13799.0
74	9750.0
75	9750.0
76	7592.0
77	5434.0
78	4421.5
79	3409.0
80	2937.0
81	2465.0
82	2465.0
83	2022.0
84	1579.0
85	1416.5
86	1254.0
87	1136.5
88	1019.0
89	1019.0
90	743.5
91	468.0
92	266.5
93	65.0
94	42.0
95	19.0
96	19.0
97	15.0
98	11.0
99	6.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1001068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.83504862559544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7184198233826	48.419271875733685
2	6.87453185613502	7.676796409310076
3	2.1866101872979296	3.6626845839900684
4	1.1139128304922667	2.4878150822084146
5	0.6369192645966941	1.7781209054667453
6	0.4182390458573821	1.4011438477541742
7	0.31089757624390835	1.2151286901010871
8	0.2164338869945493	0.966767728365383
9	0.1636786002558522	0.8225102343829416
>10	1.1182307858789395	12.145419556542183
>50	0.13318300467373162	5.264238651976103
>100	0.1014050628212648	11.151649384252517
>500	0.006820163429961169	2.5436715554456866
>1k	7.179119399959124E-4	0.46478149447098815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1271	0.1269644020186441	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1181	0.11797400376398007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1133	0.11317912469482592	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1053	0.105187659579569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.989331394071132E-5	0.0
10	0.0	0.0	0.0	1.9978662788142264E-4	0.0
11	0.0	0.0	0.0	3.995732557628453E-4	0.0
12	0.0	0.0	0.0	4.994665697035566E-4	0.0
13	0.0	0.0	0.0	4.994665697035566E-4	0.0
14	0.0	0.0	0.0	6.992531975849792E-4	0.0
15	0.0	0.0	0.0	9.989331394071133E-4	0.0
16	0.0	0.0	0.0	0.0011987197672885359	0.0
17	0.0	0.0	0.0	0.0011987197672885359	0.0
18	0.0	0.0	0.0	0.0012986130812292472	0.0
19	0.0	0.0	0.0	0.001598293023051381	0.0
20	0.0	0.0	0.0	0.002097759592754938	0.0
21	0.0	0.0	0.0	0.002797012790339917	0.0
22	0.0	0.0	0.0	0.0032964793600434735	0.0
23	0.0	0.0	0.0	0.005194452324916989	0.0
24	0.0	0.0	0.0	0.007491998545553349	0.0
25	0.0	0.0	0.0	0.009689651452248997	0.0
26	0.0	0.0	0.0	0.01668218342809879	0.0
27	0.0	0.0	0.0	0.04864804388912641	0.0
28	0.0	0.0	0.0	0.15683250288691677	0.0
29	0.0	0.0	0.0	0.3037755676937031	0.0
30	0.0	0.0	0.0	0.4931732909252918	0.0
31	0.0	0.0	0.0	0.9272097399976825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	615	0.0	25.268291	1
ACTGATC	225	0.0	21.377777	8
AACGGAG	200	0.0	21.275002	25
GTCTAGA	230	0.0	20.913044	1
ACAACGG	205	0.0	20.7561	23
TACCGAT	45	0.0038254678	20.555557	27
TAGAACT	235	0.0	20.468084	4
ACGGAGT	200	0.0	20.35	26
TCTAGCG	270	0.0	19.185186	28
GTATCAA	820	0.0	19.17683	2
CTAGCGG	275	0.0	18.836363	29
GTTCTAG	260	0.0	18.5	1
CAACGGA	230	0.0	18.5	24
TACATTG	70	1.2189976E-4	18.5	5
TAGGCTC	50	0.007034262	18.5	5
GTCTTAC	50	0.007034262	18.5	1
TAGGATC	60	9.2356786E-4	18.5	4
CTAGAAC	265	0.0	18.150944	3
CGGAGTC	235	0.0	17.319147	27
ATAATAC	65	0.0015797411	17.076923	3
>>END_MODULE