FastQCFastQC Report
Fri 10 Feb 2017
ERR1631344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592922
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT196173.3085296210968727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150932.5455287542037572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141792.39137694334162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.9957464894201936No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA28620.48269418237137435No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.3420348713658795No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.30341259052624125No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT16690.28148727825919767No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16530.27878877828786924No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA16410.2767649033093729No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT16040.27052462212567585No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.25686346602082566No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT14450.24370827866059952No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT14200.23949187245539885No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13910.23460084125736608No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA13710.23122771629320552No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA13390.22583071635054863No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.21773521643656332No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT12850.21672327894731516No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC12540.2114949352528663No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC12500.21082031026003423No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA11670.19682184165876793No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA11640.19631587291414387No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA11350.19142484171611104No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.1750651856399324No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.1743905606471003No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.1718607169239799No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA9880.16663237322953103No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT9200.1551637483513852No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT9040.15246524838005673No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT9000.15179062338722463No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT8900.15010406090514436No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8310.14015334226087073No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT8120.13694887354491822No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA7710.1300339673683891No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.12801009238989275No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA7340.1237936861846921No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA7190.12126384246157168No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG6900.11637281126353888No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.11418028003683453No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA6670.11249371755475425No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA6330.10675940511568133No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC6250.10541015513001711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT200.001841217437.01
GTCAATA405.935353E-527.751
CAGTATC2250.027.1333331
TATGCCT556.253158E-726.9090936
CAGGTAT358.864311E-426.428571
CAGTCAA358.864311E-426.428571
GCTCTAT1450.025.517241
GGTACAT851.9717845E-923.9411771
GTTGAAC701.9196523E-723.7857133
ATATGCT1300.022.7692335
TATGCTG1300.022.7692336
ATGCCCC3550.022.40845137
ATTGACT3600.022.09722131
TATTGAC3800.021.90789430
GGCAGAG1107.4578566E-1121.8636361
TGAACCA603.7223712E-521.5833325
GGGTATC6100.021.229511
TACACTG1750.021.1428595
AGTATCA3000.020.9666672
GGAGTAC1500.020.9666671
Produced by FastQC (version 0.11.5)