##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592922
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64038271475843	34.0	31.0	34.0	31.0	34.0
2	32.73213002722112	34.0	31.0	34.0	31.0	34.0
3	32.728568007258964	34.0	31.0	34.0	31.0	34.0
4	36.21623586238999	37.0	35.0	37.0	35.0	37.0
5	36.23555712218471	37.0	35.0	37.0	35.0	37.0
6	36.34705745443751	37.0	37.0	37.0	35.0	37.0
7	36.34110051575081	37.0	37.0	37.0	35.0	37.0
8	36.38149031407166	37.0	37.0	37.0	35.0	37.0
9	38.161144298912845	39.0	39.0	39.0	37.0	39.0
10	38.135203281375965	39.0	38.0	39.0	37.0	39.0
11	38.198678746951536	39.0	39.0	39.0	37.0	39.0
12	38.01080917894765	39.0	38.0	39.0	37.0	39.0
13	38.12988049018252	39.0	39.0	39.0	37.0	39.0
14	39.43959745126678	41.0	39.0	41.0	37.0	41.0
15	39.38146164250947	40.0	39.0	41.0	37.0	41.0
16	39.300069823686755	40.0	39.0	41.0	36.0	41.0
17	39.34697649943838	40.0	39.0	41.0	37.0	41.0
18	39.527841773454185	40.0	39.0	41.0	37.0	41.0
19	39.5881127703138	41.0	39.0	41.0	37.0	41.0
20	39.60898229446706	41.0	39.0	41.0	37.0	41.0
21	39.59416584306199	41.0	40.0	41.0	37.0	41.0
22	39.50196147216666	41.0	39.0	41.0	36.0	41.0
23	39.39552757361002	41.0	39.0	41.0	35.0	41.0
24	39.322710238446206	41.0	39.0	41.0	35.0	41.0
25	39.26242743564921	41.0	39.0	41.0	35.0	41.0
26	39.053929521926996	40.0	38.0	41.0	35.0	41.0
27	38.859452002118324	40.0	38.0	41.0	35.0	41.0
28	38.66091661297776	40.0	38.0	41.0	35.0	41.0
29	38.39212577708366	40.0	37.0	41.0	35.0	41.0
30	38.08578025440109	40.0	36.0	41.0	35.0	41.0
31	37.72409524355649	40.0	35.0	41.0	35.0	41.0
32	37.4113509028169	39.0	35.0	41.0	34.0	41.0
33	37.0932331740094	39.0	35.0	41.0	33.0	41.0
34	36.86623198329629	39.0	35.0	41.0	33.0	41.0
35	36.59836538364237	39.0	35.0	41.0	33.0	41.0
36	36.3273600912093	39.0	35.0	41.0	33.0	41.0
37	36.02222889351382	39.0	35.0	41.0	31.0	41.0
38	35.6871055551995	38.0	35.0	41.0	30.0	41.0
39	35.28190554575475	38.0	35.0	41.0	25.0	41.0
40	34.88256128124779	38.0	35.0	41.0	21.0	41.0
41	34.40042535105798	38.0	35.0	41.0	17.0	41.0
42	33.920723130529815	38.0	35.0	41.0	12.0	41.0
43	32.869768704821205	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	4.0
12	8.0
13	4.0
14	6.0
15	8.0
16	10.0
17	7.0
18	27.0
19	35.0
20	70.0
21	161.0
22	322.0
23	529.0
24	943.0
25	1473.0
26	2344.0
27	3648.0
28	5572.0
29	7694.0
30	9734.0
31	11224.0
32	13008.0
33	16238.0
34	21405.0
35	28204.0
36	44519.0
37	109728.0
38	119567.0
39	196421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.625957545849204	14.095614600234093	16.639962760700396	20.638465093216308
2	20.131484411102978	23.80076974711682	32.93991452501341	23.12783131676679
3	20.74927224828898	25.877940100046885	30.95516779610134	22.41761985556279
4	13.554734012230952	20.026411568469378	30.68177601775613	35.73707840154354
5	12.515642867021295	40.05147388695309	29.486171874209422	17.946711371816193
6	24.850148923467167	32.556727529084775	22.292308263144225	20.300815284303837
7	26.298737439325915	33.6750871109522	19.10706635948742	20.919109090234468
8	34.103305325152384	29.21902037704791	18.13847352602872	18.539200771770993
9	30.842168109801964	13.441059700938741	18.622179645889343	37.094592543369956
10	21.463194146953562	28.458380697629703	22.21472638896853	27.863698766448202
11	30.520709300717463	27.569056300828777	16.332165107720744	25.578069290733012
12	23.474588563082495	33.35801336432111	24.151912055885933	19.01548601671046
13	33.88540145246761	20.548065344176806	26.600632123618283	18.9659010797373
14	24.05392277567707	22.677350477803152	28.654359258047435	24.614367488472347
15	29.629529685186245	25.850786444085394	24.2016656491073	20.318018221621056
16	20.38446878341502	27.075062149827467	29.19220403358283	23.348265033174684
17	16.091829954024305	32.35197884376023	29.45294659331244	22.103244608903026
18	19.109427546962333	23.8802068400228	33.886076077460444	23.124289535554425
19	19.298153888707116	29.445694374639498	35.50332084152721	15.752830895126172
20	21.90338695477651	23.823369684376697	37.30575016612641	16.967493194720383
21	25.920104162098895	22.003231453715667	34.47215653998333	17.604507844202104
22	24.02491390098529	23.64290749879411	31.095321138362213	21.23685746185839
23	20.338425627654228	26.198892940386763	31.83605938049187	21.62662205146714
24	18.512047115809498	27.016201119202865	35.93508083693976	18.536670928047872
25	17.79053568597556	26.45288925018805	33.58401273691987	22.172562326916527
26	21.950104735530136	24.60441676982807	32.986969618263444	20.458508876378342
27	20.105174036382525	25.38360863654916	32.02174990976891	22.48946741729941
28	18.06156627684586	25.90256391228526	35.8475482441198	20.188321566749085
29	18.252822462313762	26.422531125510606	37.14653866781802	18.178107744357604
30	17.606700375428808	27.408495552534735	38.14464634471314	16.840157727323323
31	17.91871443461366	28.725363538543014	34.53675188304701	18.819170143796317
32	16.46793338752821	28.612195195995426	37.81239353574332	17.107477880733047
33	17.74752834268251	26.605523154816318	37.94478869058662	17.70215981191455
34	18.361099773663312	27.686947018326187	33.257325584140915	20.694627623869582
35	16.957205163579694	28.50223132216379	33.93802220190851	20.602541312348
36	14.627050438337589	27.826257079346018	35.30059603118117	22.246096451135227
37	15.363572274262044	27.664684393562727	35.94891064929283	21.022832682882402
38	16.019307767294855	24.432050084159467	37.6396895375783	21.90895261096738
39	16.258462327253838	24.279416179531204	38.903430805401044	20.55869068781391
40	15.587547771882305	24.262213242213985	36.88916923305258	23.261069752851135
41	12.643315646914772	25.75195388263549	39.09839742832953	22.50633304212021
42	12.613126178485533	25.130624264237117	37.99319303382232	24.263056523455024
43	11.639810970077008	27.582211488189003	38.99821561689396	21.77976192484003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	142.5
2	244.0
3	1173.0
4	2102.0
5	2102.0
6	3089.0
7	4076.0
8	4259.0
9	4442.0
10	6751.5
11	9061.0
12	9061.0
13	15003.0
14	20945.0
15	31110.5
16	41276.0
17	36368.5
18	31461.0
19	31461.0
20	32565.0
21	33669.0
22	23856.0
23	14043.0
24	11493.0
25	8943.0
26	8943.0
27	7825.5
28	6708.0
29	6576.5
30	6445.0
31	6094.5
32	5744.0
33	5744.0
34	5659.0
35	5574.0
36	5897.0
37	6220.0
38	7554.0
39	8888.0
40	8888.0
41	12156.0
42	15424.0
43	21015.0
44	26606.0
45	35291.0
46	43976.0
47	43976.0
48	56232.0
49	68488.0
50	63708.0
51	58928.0
52	45570.5
53	32213.0
54	32213.0
55	25819.0
56	19425.0
57	18649.0
58	17873.0
59	17596.5
60	17320.0
61	17320.0
62	17070.5
63	16821.0
64	16640.0
65	16459.0
66	15497.0
67	14535.0
68	14535.0
69	13206.0
70	11877.0
71	10478.0
72	9079.0
73	7641.5
74	6204.0
75	6204.0
76	5052.5
77	3901.0
78	3057.0
79	2213.0
80	1603.0
81	993.0
82	993.0
83	744.0
84	495.0
85	319.5
86	144.0
87	96.0
88	48.0
89	48.0
90	29.5
91	11.0
92	8.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	592922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.596240248693036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.66305299366958	38.64846331990905
2	7.021463136006023	6.262617138213325
3	2.055571266738287	2.7501225017931277
4	0.9965925309860381	1.7777711976762565
5	0.5755673161397704	1.2834069154932328
6	0.40833910687644354	1.0926233347919172
7	0.2993678299505918	0.9345475767044533
8	0.23148788266881284	0.8258791384127714
9	0.1846346138438243	0.7410608637483469
>10	1.2580319394136528	11.109409273965692
>50	0.16111546173783892	5.014179741962647
>100	0.12159657488232528	10.821606141258595
>500	0.01291963608124706	3.7960945077488497
>1k	0.008739753819667129	5.656945085383335
>5k	3.799892965072665E-4	1.0004973873375835
>10k+	0.0011399678895217993	8.284775875600799
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19617	3.3085296210968727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15093	2.5455287542037572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14179	2.39137694334162	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5904	0.9957464894201936	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2862	0.48269418237137435	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.3420348713658795	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1799	0.30341259052624125	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	1669	0.28148727825919767	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1653	0.27878877828786924	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1641	0.2767649033093729	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	1604	0.27052462212567585	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1523	0.25686346602082566	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	1445	0.24370827866059952	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	1420	0.23949187245539885	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1391	0.23460084125736608	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1371	0.23122771629320552	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	1339	0.22583071635054863	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.21773521643656332	No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	1285	0.21672327894731516	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	1254	0.2114949352528663	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	1250	0.21082031026003423	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	1167	0.19682184165876793	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	1164	0.19631587291414387	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	1135	0.19142484171611104	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1038	0.1750651856399324	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.1743905606471003	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.1718607169239799	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	988	0.16663237322953103	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	920	0.1551637483513852	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	904	0.15246524838005673	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	900	0.15179062338722463	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	890	0.15010406090514436	No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	831	0.14015334226087073	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	812	0.13694887354491822	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	771	0.1300339673683891	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.12801009238989275	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	734	0.1237936861846921	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	719	0.12126384246157168	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	690	0.11637281126353888	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.11418028003683453	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	667	0.11249371755475425	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	633	0.10675940511568133	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	625	0.10541015513001711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6865624820802737E-4	1.6865624820802737E-4
2	0.0	0.0	0.0	1.6865624820802737E-4	1.6865624820802737E-4
3	0.0	0.0	0.0	1.6865624820802737E-4	3.3731249641605473E-4
4	0.0	0.0	0.0	1.6865624820802737E-4	3.3731249641605473E-4
5	0.0	0.0	0.0	3.3731249641605473E-4	3.3731249641605473E-4
6	0.0	0.0	0.0	3.3731249641605473E-4	3.3731249641605473E-4
7	0.0	0.0	0.0	3.3731249641605473E-4	3.3731249641605473E-4
8	0.0	0.0	0.0	3.3731249641605473E-4	3.3731249641605473E-4
9	0.0	0.0	0.0	3.3731249641605473E-4	3.3731249641605473E-4
10	0.0	0.0	0.0	5.059687446240821E-4	0.0010119374892481642
11	0.0	0.0	0.0	0.0011805937374561916	0.0010119374892481642
12	0.0	0.0	0.0	0.0020238749784963284	0.0010119374892481642
13	0.0	0.0	0.0	0.0021925312267043555	0.0010119374892481642
14	0.0	0.0	0.0	0.0030358124677444926	0.0011805937374561916
15	0.0	0.0	0.0	0.00573431243907293	0.0011805937374561916
16	0.0	0.0	0.0	0.01349249985664219	0.001349249985664219
17	0.0	0.0	0.0	0.02310590600449975	0.001349249985664219
18	0.0	0.0	0.0	0.02884021844357268	0.001349249985664219
19	0.0	0.0	0.0	0.033731249641605475	0.001349249985664219
20	0.0	0.0	0.0	0.03963421832888643	0.0015179062338722463
21	0.0	0.0	0.0	0.050259561965992156	0.0015179062338722463
22	0.0	0.0	0.0	0.0659445930493387	0.0015179062338722463
23	0.0	0.0	0.0	0.09292959276262308	0.0015179062338722463
24	0.0	0.0	0.0	0.13829812353058243	0.0015179062338722463
25	0.0	0.0	0.0	0.15701896708167348	0.0016865624820802737
26	0.0	0.0	0.0	0.18619649802166222	0.0016865624820802737
27	0.0	0.0	0.0	0.2706932783738839	0.0016865624820802737
28	0.0	0.0	0.0	0.560444712795275	0.0020238749784963284
29	0.0	0.0	0.0	1.0627030199587804	0.0020238749784963284
30	0.0	0.0	0.0	1.8730962925983519	0.0021925312267043555
31	0.0	0.0	0.0	3.4432859634150867	0.0025298437231204103
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	20	0.0018412174	37.0	1
GTCAATA	40	5.935353E-5	27.75	1
CAGTATC	225	0.0	27.133333	1
TATGCCT	55	6.253158E-7	26.90909	36
CAGGTAT	35	8.864311E-4	26.42857	1
CAGTCAA	35	8.864311E-4	26.42857	1
GCTCTAT	145	0.0	25.51724	1
GGTACAT	85	1.9717845E-9	23.941177	1
GTTGAAC	70	1.9196523E-7	23.785713	3
ATATGCT	130	0.0	22.76923	35
TATGCTG	130	0.0	22.76923	36
ATGCCCC	355	0.0	22.408451	37
ATTGACT	360	0.0	22.097221	31
TATTGAC	380	0.0	21.907894	30
GGCAGAG	110	7.4578566E-11	21.863636	1
TGAACCA	60	3.7223712E-5	21.583332	5
GGGTATC	610	0.0	21.22951	1
TACACTG	175	0.0	21.142859	5
AGTATCA	300	0.0	20.966667	2
GGAGTAC	150	0.0	20.966667	1
>>END_MODULE