FastQCFastQC Report
Fri 10 Feb 2017
ERR1631343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271163
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.38095167851071127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8510.31383337697252206No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7190.2651541692635057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.12169801927254087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA555.129178E-420.1818187
GGTATCA4350.019.9885061
CTATAAT500.007021364818.51
CCGTCGT500.007021364818.59
GAACACT701.2148431E-418.56
TACCCTA1301.3793397E-817.0769235
TCGGTGA650.00157572917.07692313
CTATTCC1005.844542E-616.6500024
ACAGTGC803.3706563E-416.18758
ATGGTCG803.3706563E-416.18755
GAGCGAA700.002585650215.85714216
CGATGCG700.002585650215.85714233
ATAACGC700.002585650215.8571423
CAGTCGG700.002585650215.85714210
ACCCGTT1302.5675035E-715.65384630
CGCGGGT1455.2930773E-815.31034622
CCCGTTG1353.9393854E-715.07407531
GCGGGTA1353.9393854E-715.07407523
CCGGCAG1508.021743E-814.814
CGGTGAT750.004093660514.814
Produced by FastQC (version 0.11.5)